plaP:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

plaP

Mnemonic

Systematic nomenclature

Synonyms

ECK2008, b2014, JW5330[1], yeeF, JW5330

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

44.91 minutes 

MG1655: 2085086..2083728
<gbrowseImage> name=NC_000913:2083728..2085086 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2017505..2016147
<gbrowseImage> name=NC_012967:2016147..2017505 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1976211..1977569
<gbrowseImage> name=NC_012759:1976211..1977569 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2089199..2087841
<gbrowseImage> name=NC_007779:2087841..2089199 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2176094..2174736
<gbrowseImage> name=NC_010473:2174736..2176094 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yeeF(del) (Keio:JW5330)

deletion

deletion

PMID:16738554[2]

Shigen

CGSC11323[3]

yeeF::Tn5KAN-I-SceI (FB20572)

Insertion at nt 544 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20572

contains pKD46

yeeF783(del)::kan

PMID:16738554[2]

CGSC:104498

yeef(del)

deletion

deletion

Single knock-out mutant shows impaired putrescine uptake, fig 5.

PMID:21266585[5]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5330

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCGCATAACGTTACTCCAAA

Primer 2:CCTGCTACGTCTTCGTACTGCGG

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[3]

est. P1 cotransduction: 98% [7]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: % [7]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11896

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11896

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001826

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946533

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1842

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006696

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kurihara, S et al. (2011) A novel putrescine importer required for type 1 pili-driven surface motility induced by extracellular putrescine in Escherichia coli K-12. J. Biol. Chem. 286 10185-92 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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