parC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

parC

Gene Synonym(s)

ECK3010, b3019, JW2987[1], JW2987

Product Desc.

topoisomerase IV subunit A[2][3];

Component of Topoisomerase IV[3]

Topoisomerase IV, subunit A, ATP-dependent, type II; chromosome decatenase; relaxes both positive and negative supercoils; DNA unknotting activity; heterotetrameric[4]

Product Synonyms(s)

DNA topoisomerase IV, subunit A[1], B3019[2][1], ParC[2][1] , ECK3010, JW2987, b3019

Category:Complex:topoisomerase IV

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): parC[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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ParC physically interacts with SeqA. Topo IV is stimulated by SeqA.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

parC

Mnemonic

partitioning of the nucleoid Partition

Synonyms

ECK3010, b3019, JW2987[1], JW2987

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

68.15 minutes 

MG1655: 3163995..3161737
<gbrowseImage> name=NC_000913:3161737..3163995 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3100145..3097887
<gbrowseImage> name=NC_012967:3097887..3100145 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3048885..3051143
<gbrowseImage> name=NC_012759:3048885..3051143 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3164629..3162371
<gbrowseImage> name=NC_007779:3162371..3164629 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3261740..3259482
<gbrowseImage> name=NC_010473:3259482..3261740 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

parC1215

G2199A

G703D

Growth Phenotype

  • ts @ 42
  • Chromosome partitioning phenotype
  • Filamentation

PMID:2170028, PMID:2842295

actual nucleotide mapping was referenced in [5]

parC10

Y120H

DNA nicking independent of ATP hydrolysis

PMID:9077455

parCL80

S80L

Resistant to

Quinolone resistance

PMID:8524852

The mutation conferred increased resistance toward quinolones, specifically norfloxcin. See figure 3. chEBI:23765

parCL80 gyrAr

parC S80L gyrA S83L

Resistant to

quinolone resistance

PMID:8524852

The double mutation conferred a 6 fold increase of quinolone resistance compared to the mutation with only the gyrAR mutation. See figure 4 for experimental results. chEBI:23765

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Notes

  • The partitioning defect comes from the inability of these cells to partition the nucleoids into daughter cells. This results in filamentous cells with a cluster of nucleoid at the center and subsequent DNA-free cells.
  • The Keio collection[6] lists a deletion of parC, which is an essential gene. There is a duplication of the parC region.[7].
  • Suppressors of the parC1215 mutation when expressed at high-copy[8]:
    • topB
    • dnaN (β subunit of the DNA polymerase holoenzyme)
    • spcA (suppressor of parC A- will be renamed setB)


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2987

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGCGATATGGCAGAGCGCCT

Primer 2:CCtTCTTCGCTATCACCGCTGCT

6H4

Kohara Phage

Genobase

PMID:3038334

17B2

Kohara Phage

Genobase

PMID:3038334

metC162::Tn10

Linked marker

CAG18475 = CGSC7435[9]

est. P1 cotransduction: 67% [10]

tolC210::Tn10

Linked marker

CAG12184 = CGSC7437[9]

est. P1 cotransduction: 56% [10]

plexBA-parEC

Plasmid Clone

  • IPTG-inducible Topo IV expression plasmid[11]

pLC4-14

Plasmid Clone

plasmid from the Clarke-Carbon collection

PMID:2842295

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10686

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10686

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000679

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947499

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0680

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009916

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ParC

Synonyms

DNA topoisomerase IV, subunit A[1], B3019[2][1], ParC[2][1] , ECK3010, JW2987, b3019

Category:Complex:topoisomerase IV

Product description

topoisomerase IV subunit A[2][3];

Component of Topoisomerase IV[3]

Topoisomerase IV, subunit A, ATP-dependent, type II; chromosome decatenase; relaxes both positive and negative supercoils; DNA unknotting activity; heterotetrameric[4]

EC number (for enzymes)

5.99.1.-[1]

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Notes

More information about the Topo IV complex may be found on the Topoisomerase IV page.

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

IDA: Inferred from Direct Assay

F

Missing: reference

GO:0007059

chromosome segregation

PMID:2842295

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002205

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0003916

DNA topoisomerase activity

PMID:1334483

IDA: Inferred from Direct Assay

F

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005742

F

Seeded from EcoCyc (v14.0)

complete

GO:0019897

extrinsic to plasma membrane

PMID:1334483

IDA: Inferred from Direct Assay

C

in the presence of DNA

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006691

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:1334483

IDA: Inferred from Direct Assay

C

in the presence of Mg2+

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013757

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

PMID:8227000

IDA: Inferred from Direct Assay

F

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013758

F

Seeded from EcoCyc (v14.0)

complete

GO:0009330

DNA topoisomerase complex (ATP-hydrolyzing)

PMID:8227000

IDA: Inferred from Direct Assay

C

complete

GO:0006265

DNA topological change

PMID:8227000

IDA: Inferred from Direct Assay

P

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013760

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:16023670

IDA: Inferred from Direct Assay

F

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003916

DNA topoisomerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006691

F

Seeded from EcoCyc (v14.0)

complete

GO:0030541

plasmid partitioning

PMID:8104339

IDA: Inferred from Direct Assay

P

complete

GO:0003916

DNA topoisomerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0799

F

Seeded from EcoCyc (v14.0)

complete

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002205

F

Seeded from EcoCyc (v14.0)

complete

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005742

F

Seeded from EcoCyc (v14.0)

complete

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013757

F

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

PMID:16023670

IDA: Inferred from Direct Assay

P

complete

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013758

F

Seeded from EcoCyc (v14.0)

complete

GO:0007059

chromosome segregation

PMID:16023670

IDA: Inferred from Direct Assay

P

complete

GO:0003918

DNA topoisomerase (ATP-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013760

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002205

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005742

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006691

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013757

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013758

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013760

F

Seeded from EcoCyc (v14.0)

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002205

C

Seeded from EcoCyc (v14.0)

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005742

C

Seeded from EcoCyc (v14.0)

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006691

C

Seeded from EcoCyc (v14.0)

complete

GO:0007062

sister chromatid cohesion

PMID:18765793

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013757

C

Seeded from EcoCyc (v14.0)

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013758

C

Seeded from EcoCyc (v14.0)

complete

GO:0005694

chromosome

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013760

C

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002205

P

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005742

P

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006691

P

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013757

P

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013758

P

Seeded from EcoCyc (v14.0)

complete

GO:0006265

DNA topological change

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013760

P

Seeded from EcoCyc (v14.0)

complete

GO:0016853

isomerase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0413

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of dimer of topoisomerase IV subunit A

Protein

yciH

PMID:16606699

Experiment(s):EBI-1144556

Protein

rplB

PMID:16606699

Experiment(s):EBI-1144556

Protein

rplA

PMID:16606699

Experiment(s):EBI-1144556

Protein

pflD

PMID:16606699

Experiment(s):EBI-1144556

Protein

aceF

PMID:15690043

Experiment(s):EBI-883350

Protein

lpdA

PMID:15690043

Experiment(s):EBI-883350

Protein

mreB

PMID:15690043

Experiment(s):EBI-883350

Protein

gyrA

PMID:15690043

Experiment(s):EBI-883350

Protein

secA

PMID:15690043

Experiment(s):EBI-883350

Protein

murF

PMID:15690043

Experiment(s):EBI-890645

Protein

rfaQ

PMID:15690043

Experiment(s):EBI-890645

Protein

rplL

PMID:15690043

Experiment(s):EBI-890645

Protein

rplM

PMID:15690043

Experiment(s):EBI-890645

Protein

rplV

PMID:15690043

Experiment(s):EBI-890645

Protein

rpmA

PMID:15690043

Experiment(s):EBI-890645

Protein

rpmG

PMID:15690043

Experiment(s):EBI-890645

Protein

rpsB

PMID:15690043

Experiment(s):EBI-890645

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-890645

Protein

slyD

PMID:15690043

Experiment(s):EBI-890645

Protein

murF

PMID:19402753

LCMS(ID Probability):99.0

Protein

mreB

PMID:19402753

MALDI(Z-score):33.310369

Protein

rplB

PMID:19402753

MALDI(Z-score):37.621172

Protein

gyrA

PMID:19402753

MALDI(Z-score):26.844062

Protein

rfaQ

PMID:19402753

LCMS(ID Probability):99.0

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of topoisomerase IV

could be indirect

Protein

MreB

PMID:19187760

Purified MreB inhibits decatanation by Topo IV.

Protein

FtsK

FtsK stimulates Topo IV's decatenation activity in vitro

PMID:12939258

  • Two-hybrid analysis
  • Immunoblotting

Protein

MukB

PMID:20696938


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

cytoplasm

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS ELGLNASAKF
KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV DGQGNWGAPD DPKSFAAMRY
TESRLSKYSE LLLSELGQGT ADWVPNFDGT LQEPKMLPAR LPNILLNGTT GIAVGMATDI
PPHNLREVAQ AAIALIDQPK TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR
MRAVWKKEDG AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV
PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW LVFRRDTVRR
RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK PALMSRFGLT ETQAEAILEL
KLRHLAKLEE MKIRGEQSEL EKERDQLQGI LASERKMNNL LKKELQADAQ AYGDDRRSPL
QEREEAKAMS EHDMLPSEPV TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN
QPVVFVDSTG RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA
GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG RMLMFPVSDL
PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI HVGKRKIKLR PEELQKVTGE
RGRRGTLMRG LQRIDRVEID SPRRASSGDS EE
Length

752

Mol. Wt

83.833 kDa

pI

6.7 (calculated)

Extinction coefficient

55,810 - 56,060 (calc based on 19 Y, 5 W, and 2 C residues)

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<wikiPageContents>Image:1ZVU.pdb</wikiPageContents>



Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

591..638

PF03989 DNA gyrase C-terminal domain, beta-propeller

PMID:19920124

Domain

640..691

PF03989 DNA gyrase C-terminal domain, beta-propeller

PMID:19920124

Domain

29..466

PF00521 DNA gyrase/topoisomerase IV, subunit A

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=parC taxon=562,83333 </beststructure>

Models

View models at:

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See Help:Jmol in EcoliWiki

Text label
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents >1ZVU.pdb</uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

Purification protocol

ParC(ΔCTD)

PMID:20081205

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130915

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947499

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009916

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AFI2

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10686

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10686

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947499

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000679

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0680

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.74E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

25.585+/-0.158

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.05425155

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1554

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

320

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

891

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

parC

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3163975..3164015 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3019 (EcoliWiki Page)

NCBI GEO profiles for parC

microarray

GenExpDB:b3019 (EcoliWiki Page)

Summary of data for parC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (3163897..3164188) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ09; Well:G12[12]

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Notes

Accessions Related to parC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10686

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0680

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3019

EcoGene

EcoGene:EG10686

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000679

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009916

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000029476 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000025654 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000011728 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

PARC

From SHIGELLACYC

E. coli O157

PARC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00521 DNA gyrase/topoisomerase IV, subunit A

Pfam (EcoliWiki Page)

PF03989 DNA gyrase C-terminal domain, beta-propeller

Pfam (EcoliWiki Page)

PF03989 DNA gyrase C-terminal domain, beta-propeller

Panther (EcoliWiki Page)

PTHR10169:SF4

Superfamily (EcoliWiki Page)

SUPERFAMILY:56719

Superfamily (EcoliWiki Page)

SUPERFAMILY:101904

EcoCyc

EcoCyc:EG10686

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10686

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000679

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0680

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009916

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Kato, J et al. (1990) New topoisomerase essential for chromosome segregation in E. coli. Cell 63 393-404 PubMed
  6. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  7. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  8. ↑ Nurse, P et al. (2003) Topoisomerase III can serve as the cellular decatenase in Escherichia coli. J. Biol. Chem. 278 8653-60 PubMed
  9. ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  11. ↑ Mossessova, E et al. (2000) Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative parE alleles. J. Biol. Chem. 275 4099-103 PubMed
  12. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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