parC:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
ParC |
|---|---|
| Synonyms |
DNA topoisomerase IV, subunit A[1], B3019[2][1], ParC[2][1] , ECK3010, JW2987, b3019 Category:Complex:topoisomerase IV |
| Product description |
topoisomerase IV subunit A[2][3]; Component of Topoisomerase IV[3] Topoisomerase IV, subunit A, ATP-dependent, type II; chromosome decatenase; relaxes both positive and negative supercoils; DNA unknotting activity; heterotetrameric[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
More information about the Topo IV complex may be found on the Topoisomerase IV page.
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
IDA: Inferred from Direct Assay |
F |
Missing: reference | ||||
| GO:0007059 |
chromosome segregation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Contributes to |
GO:0003916 |
DNA topoisomerase activity |
IDA: Inferred from Direct Assay |
F |
complete | |||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0019897 |
extrinsic to plasma membrane |
IDA: Inferred from Direct Assay |
C |
in the presence of DNA |
complete | |||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
in the presence of Mg2+ |
complete | |||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Contributes to |
GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
IDA: Inferred from Direct Assay |
F |
complete | |||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009330 |
DNA topoisomerase complex (ATP-hydrolyzing) |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0006265 |
DNA topological change |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003916 |
DNA topoisomerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0030541 |
plasmid partitioning |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003916 |
DNA topoisomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007059 |
chromosome segregation |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003918 |
DNA topoisomerase (ATP-hydrolyzing) activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0007062 |
sister chromatid cohesion |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005694 |
chromosome |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006265 |
DNA topological change |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016853 |
isomerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of dimer of topoisomerase IV subunit A |
|||
|
Protein |
yciH |
Experiment(s):EBI-1144556 | ||
|
Protein |
rplB |
Experiment(s):EBI-1144556 | ||
|
Protein |
rplA |
Experiment(s):EBI-1144556 | ||
|
Protein |
pflD |
Experiment(s):EBI-1144556 | ||
|
Protein |
aceF |
Experiment(s):EBI-883350 | ||
|
Protein |
lpdA |
Experiment(s):EBI-883350 | ||
|
Protein |
mreB |
Experiment(s):EBI-883350 | ||
|
Protein |
gyrA |
Experiment(s):EBI-883350 | ||
|
Protein |
secA |
Experiment(s):EBI-883350 | ||
|
Protein |
murF |
Experiment(s):EBI-890645 | ||
|
Protein |
rfaQ |
Experiment(s):EBI-890645 | ||
|
Protein |
rplL |
Experiment(s):EBI-890645 | ||
|
Protein |
rplM |
Experiment(s):EBI-890645 | ||
|
Protein |
rplV |
Experiment(s):EBI-890645 | ||
|
Protein |
rpmA |
Experiment(s):EBI-890645 | ||
|
Protein |
rpmG |
Experiment(s):EBI-890645 | ||
|
Protein |
rpsB |
Experiment(s):EBI-890645 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-890645 | ||
|
Protein |
slyD |
Experiment(s):EBI-890645 | ||
|
Protein |
murF |
LCMS(ID Probability):99.0 | ||
|
Protein |
mreB |
MALDI(Z-score):33.310369 | ||
|
Protein |
rplB |
MALDI(Z-score):37.621172 | ||
|
Protein |
gyrA |
MALDI(Z-score):26.844062 | ||
|
Protein |
rfaQ |
LCMS(ID Probability):99.0 | ||
|
Protein |
rplL |
LCMS(ID Probability):99.6 | ||
|
Protein |
Subunits of topoisomerase IV |
could be indirect |
||
|
Protein |
MreB |
Purified MreB inhibits decatanation by Topo IV. | ||
|
Protein |
FtsK |
FtsK stimulates Topo IV's decatenation activity in vitro |
| |
|
Protein |
MukB |
| ||
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
cytoplasm |
From EcoCyc[3] |
| ||
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS ELGLNASAKF KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV DGQGNWGAPD DPKSFAAMRY TESRLSKYSE LLLSELGQGT ADWVPNFDGT LQEPKMLPAR LPNILLNGTT GIAVGMATDI PPHNLREVAQ AAIALIDQPK TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR MRAVWKKEDG AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW LVFRRDTVRR RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK PALMSRFGLT ETQAEAILEL KLRHLAKLEE MKIRGEQSEL EKERDQLQGI LASERKMNNL LKKELQADAQ AYGDDRRSPL QEREEAKAMS EHDMLPSEPV TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN QPVVFVDSTG RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG RMLMFPVSDL PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI HVGKRKIKLR PEELQKVTGE RGRRGTLMRG LQRIDRVEID SPRRASSGDS EE |
| Length |
752 |
| Mol. Wt |
83.833 kDa |
| pI |
6.7 (calculated) |
| Extinction coefficient |
55,810 - 56,060 (calc based on 19 Y, 5 W, and 2 C residues) |
| edit table |
<wikiPageContents>Image:1ZVU.pdb</wikiPageContents>
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | |||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
|
Purification protocol |
ParC(ΔCTD) |
|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Kato, J et al. (1988) Gene organization in the region containing a new gene involved in chromosome partition in Escherichia coli. J. Bacteriol. 170 3967-77 PubMed
- ↑ 6.0 6.1 6.2 Kato, J et al. (1992) Purification and characterization of DNA topoisomerase IV in Escherichia coli. J. Biol. Chem. 267 25676-84 PubMed
- ↑ 7.0 7.1 7.2 Peng, H & Marians, KJ (1993) Escherichia coli topoisomerase IV. Purification, characterization, subunit structure, and subunit interactions. J. Biol. Chem. 268 24481-90 PubMed
- ↑ 8.0 8.1 8.2 Corbett, KD et al. (2005) The structural basis for substrate specificity in DNA topoisomerase IV. J. Mol. Biol. 351 545-61 PubMed
- ↑ Peng, H & Marians, KJ (1993) Decatenation activity of topoisomerase IV during oriC and pBR322 DNA replication in vitro. Proc. Natl. Acad. Sci. U.S.A. 90 8571-5 PubMed
- ↑ Wang, X et al. (2008) Modulation of Escherichia coli sister chromosome cohesion by topoisomerase IV. Genes Dev. 22 2426-33 PubMed
- ↑ 11.0 11.1 11.2 11.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 12.00 12.01 12.02 12.03 12.04 12.05 12.06 12.07 12.08 12.09 12.10 12.11 12.12 12.13 12.14 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 13.0 13.1 13.2 13.3 13.4 13.5 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Madabhushi, R & Marians, KJ (2009) Actin homolog MreB affects chromosome segregation by regulating topoisomerase IV in Escherichia coli. Mol. Cell 33 171-80 PubMed
- ↑ Espeli, O et al. (2003) A physical and functional interaction between Escherichia coli FtsK and topoisomerase IV. J. Biol. Chem. 278 44639-44 PubMed
- ↑ Hayama, R & Marians, KJ (2010) Physical and functional interaction between the condensin MukB and the decatenase topoisomerase IV in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 107 18826-31 PubMed
- ↑ 17.0 17.1 17.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
- ↑ Bigot, S & Marians, KJ (2010) DNA chirality-dependent stimulation of topoisomerase IV activity by the C-terminal AAA+ domain of FtsK. Nucleic Acids Res. 38 3031-40 PubMed
Categories
- GO:0003918 ! DNA topoisomerase type II (ATP-hydrolyzing) activity
- GO:0007059 ! chromosome segregation
- GO:0003677 ! DNA binding
- GO:0003916 ! DNA topoisomerase activity
- GO:0019897 ! extrinsic component of plasma membrane
- GO:0005737 ! cytoplasm
- GO:0009330 ! DNA topoisomerase complex (ATP-hydrolyzing)
- GO:0006265 ! DNA topological change
- GO:0030541 ! plasmid partitioning
- GO:0005524 ! ATP binding
- GO:0005694 ! chromosome
- GO:0007062 ! sister chromatid cohesion
- GO:0016853 ! isomerase activity
- Proteins
