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Standard Name	ompT
Gene Synonym(s)	ECK0557, b0565, JW0554^[1], JW0554
Product Desc.	outer membrane protein 3b (a), protease VII^[2]; outer membrane protease VII (outer membrane protein 3b)^[3] Outer membrane protease VII, DLP12 prophage; OM protein 2b; omptin^[4]
Product Synonyms(s)	DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)^[1], B0565^[2]^[1], OmpT^[2]^[1], protease VII^[2]^[1], omptin^[2]^[1] , ECK0557, JW0554, b0565
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): ompT^[2], envY-ompT^[2]
Regulation/Activity
Quick Links
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Notes

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Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced-expression subset.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	ompT
Mnemonic	Outer membrane protein
Synonyms	ECK0557, b0565, JW0554^[1], JW0554
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	12.58 minutes	MG1655: 584856..583903 <gbrowseImage> name=NC_000913:583903..584856 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 560984..560031 <gbrowseImage> name=NC_012967:560031..560984 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 486663..487616 <gbrowseImage> name=NC_012759:486663..487616 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 584856..583903 <gbrowseImage> name=NC_007779:583903..584856 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 524188..523235 <gbrowseImage> name=NC_010473:523235..524188 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 637448..636495 <gbrowseImage> name=NC_010473:636495..637448 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	583906	Edman degradation	PMID:3056908 PMID:3278297 PMID:9629924
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔompT (Keio:JW0554)	deletion	deletion		PMID:16738554	Shigen CGSC8680^[5]
ompTD103A		D103A	Loss of activity			seeded from UniProt:P09169
ompTD105A		D105A	Loss of activity			seeded from UniProt:P09169
ompTD230A		D230A	Loss of activity			seeded from UniProt:P09169
ompTGK236KG		GK236KG	70% of wild-type enzymatic activity			seeded from UniProt:P09169
ompTK237T		K237T	40% of wild-type enzymatic activity			seeded from UniProt:P09169
ompTR238L		R238L	Loss of activity			seeded from UniProt:P09169
ΔompT	deletion	deletion	lack ompT protease activity. lack the ability to cleave between specific paired basic amino acids	PMID: 17880914
ΔompT504					CGSC:28510
ompT1000::kan					CGSC:30156
ΔompT774::kan				PMID:16738554	CGSC:102186
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0554	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGGGCGAAACTTCTGGGAAT Primer 2:CCAAATGTGTACTTAAGACCAGC
23E10	Kohara Phage	Genobase	PMID:3038334
12A1	Kohara Phage	Genobase	PMID:3038334
2F5	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 28% ^[6] Synonyms:purE79::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10673	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10673	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000666	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945185	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001931	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	OmpT
Synonyms	DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)^[1], B0565^[2]^[1], OmpT^[2]^[1], protease VII^[2]^[1], omptin^[2]^[1] , ECK0557, JW0554, b0565
Product description	outer membrane protein 3b (a), protease VII^[2]; outer membrane protease VII (outer membrane protein 3b)^[3] Outer membrane protease VII, DLP12 prophage; OM protein 2b; omptin^[4]
EC number (for enzymes)	3.4.21.87^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0031230	intrinsic to cell outer membrane				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004175	endopeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000036	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004175	endopeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020079	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004190	aspartic-type endopeptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0064	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000036	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020079	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008233	peptidase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0645	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000036	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020079	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016787	hydrolase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc (v14.0)	complete
	GO:0031230	intrinsic to cell outer membrane	PMID:6337119	IDA: Inferred from Direct Assay		C		complete
	GO:0004175	endopeptidase activity	PMID:3277950	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0006508	proteolysis	PMID:3277950	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0031230	intrinsic to cell outer membrane	PMID:10692590	IDA: Inferred from Direct Assay		C		complete
	GO:0004252	serine-type endopeptidase activity	PMID:10692590	IMP: Inferred from Mutant Phenotype		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
outer membrane
Outer Membrane	PMID:9298646	EchoLocation:ompT
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRAKLLGIVL TTPIAISSFA STETLSFTPD NINADISLGT LSGKTKERVY LAEEGGRKVS QLDWKFNNAA IIKGAINWDL MPQISIGAAG WTTLGSRGGN MVDQDWMDSS NPGTWTDESR HPDTQLNYAN EFDLNIKGWL LNEPNYRLGL MAGYQESRYS FTARGGSYIY SSEEGFRDDI GSFPNGERAI GYKQRFKMPY IGLTGSYRYE DFELGGTFKY SGWVESSDND EHYDPGKRIT YRSKVKDQNY YSVAVNAGYY VTPNAKVYVE GAWNRVTNKK GNTSLYDHNN NTSDYSKNGA GIENYNFITT AGLKYTF
Length	317
Mol. Wt	35.563 kDa
pI	5.8 (calculated)
Extinction coefficient	78,270 (calc based on 23 Y, 8 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-20	UniProt Manual:Signal Peptides	UniProt:P09169
Domain	20..317	PF01278 Omptin family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ompT taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128548	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945185	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001931	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P09169	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10673	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10673	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945185	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000666	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0667	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.59E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	40237	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	24644	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	32467	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
ompT
envY-ompT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:584836..584876 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0565 (EcoliWiki Page)	NCBI GEO profiles for ompT
microarray	GenExpDB:b0565 (EcoliWiki Page)	Summary of data for ompT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (584578..585222) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:H2^[7]
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Notes

Accessions Related to ompT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10673	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0667	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0565
EcoGene	EcoGene:EG10673	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000666	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001931	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01278 Omptin family
Superfamily (EcoliWiki Page)	SUPERFAMILY:69917
EcoCyc	EcoCyc:EG10673	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10673	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000666	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0667	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001931	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

ompT:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to ompT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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