ompR:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
ompR |
---|---|
Gene Synonym(s) |
ECK3392, b3405, JW3368, cry, ompB, kmt[1], kmt |
Product Desc. |
Component of OmpR transcriptional dual regulator[2][3] Response regulator for osmoregulation; regulates production of outer membrane proteins[4] |
Product Synonyms(s) |
DNA-binding response regulator in two-component regulatory system with EnvZ[1], B3405[2][1], Cry[2][1], OmpB[2][1], OmpR[2][1], response regulator (sensor, EnvZ) affecting transcription of ompC and ompF: outer membrane protein synthesis[2][1], transcriptional regulatory protein OmpR[2][1], unphosphorylated transcriptional regulatory protein OmpR[2][1], unmodified transcriptional regulatory protein OmpR[2][1], Kmt[2][1] , ECK3392, JW3368, kmt, ompB, b3405 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
The cognate sensor kinase for OmpR is EnvZ. Binds TrxA (Kumar, 2004). Mutants have elevated OM vesiculation (McBroom, 2006).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
ompR |
---|---|
Mnemonic |
Outer membrane protein |
Synonyms |
ECK3392, b3405, JW3368, cry, ompB, kmt[1], kmt |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
76.17 minutes |
MG1655: 3534606..3533887 |
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NC_012967: 3465268..3464549 |
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NC_012759: 3421044..3421763 |
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W3110 |
|
W3110: 4103832..4104551 |
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DH10B: 3632351..3631632 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ompR(del) (Keio:JW3368) |
deletion |
deletion |
PMID:16738554 |
||||
ompR::Tn5KAN-I-SceI (FB21168) |
Insertion at nt 434 in Plus orientation |
PMID:15262929 |
does not contain pKD46 | ||||
ompR::Tn5KAN-I-SceI (FB21169) |
Insertion at nt 434 in Plus orientation |
PMID:15262929 |
contains pKD46 | ||||
ompR(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
PMID:16095938 |
|||
ompR(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
PMID:16095938 |
|||
ompR321(Am, polar) |
polar |
PMID:3157679 |
amber (UAG) mutation | ||||
ompR739(del)::kan |
PMID:16738554 |
||||||
ompR101 |
164-182 |
inframe deletion |
Growth Phenotype |
produces no protein even though ompR mRNA is present |
PMID:1695892 |
||
ompR1 |
deletion |
deletion of amino acids 164-182 near the C-terminus |
lost proteins |
lack both OmpF and OmpC proteins |
PMID:3010044 PMID:6799653 |
||
ompR3 |
point mutation at N-terminal |
at amino acid 15 (C43 to T) |
increased protein expression |
OmpC expression is significant even in low-osmolarity medium |
PMID:3010044 |
||
ompR4 |
point mutation |
C211 is changed to T at amino acid 71 |
protein expression |
Expression of OmpC was significant even in low-osmolarity medium |
PMID:3010044 |
||
ompR20 |
point mutation at the C-terminal |
C448 is changed to T at amino acid 150 |
protein expression |
OmpF expression was significant in high-osmolarity medium |
PMID:3010044 |
||
ompR2 |
point mutation at the C-terminal |
G607 was changed to A at amino acid 203 |
protein expression |
OmpF expression was significant in high-osmolarity medium |
PMID:3010044 PMID:6799653 |
||
ompR77 |
point mutation |
Leu-to-Gln at amino acid 16 from the N-terminus of OmpR |
protein expression |
Suppresses envZ11 pleiotropic defects |
PMID:3536870 |
||
ompRres |
protein expresison |
ompF expression increases with increasing osmolarity and ompC expression has no apparent change |
PMID:6378888 |
||||
ompR(del) |
deletion |
Change in the expression of a gene |
Decrease in the expression of rybB gene. |
PMID:17416652 |
See Figure 1 A. | ||
ompR in strain 834 |
Resistant to |
chlormaphenicol resistant |
PMID:346560 |
Strain: 834 |
|||
ompR in strain 834 |
Resistant to |
copper resistance |
PMID:346560 |
Strain: 834 |
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ompR in strain CS197 |
Resistant to |
Resistant to colicin A |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to colicin E2 |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to colicin E3 |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to colicin K |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to colicin L |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to colicin S4 |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
partially Resistant to colicin X |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to Me1 |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to hy2 |
PMID:346560 |
CS197 |
Table 2 | ||
ompR in strain CS197 |
Resistant to |
Resistant to Tulb |
PMID:346560 |
CS197 |
Table 2 | ||
ompB1422 |
Resistant to |
Resistance to beta lactam Cefoxitin |
PMID:6760806 |
Strains: B1436, B1437 (omp1350), pop1389 (ompB101) |
Table 3. | ||
ompB1422 |
Resistant to |
Resistance to Beta Lactam Ampicillin. |
PMID:6760806 |
Strains: B1436, B1437 (omp1350), pop1389 (ompB101) |
Table 3. | ||
ompR- |
Resistant to |
Small increase in Microcin B17 resistance |
PMID:3543211 |
See table 4 for results of level of resistance test. | |||
ompR-'recA - |
Resistant to |
Double mutation caused partial increase in Microcin B17 resistance |
PMID:3543211 |
See table 4 for results of resistance experiment. | |||
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW3368 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCCAAGAGAACTACAAGATTCT Primer 2:CCTGCTTTAGAGCCGTCCGGTAC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 30% [6] | ||
Linked marker |
est. P1 cotransduction: 32% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000665 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0666 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0011110 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
OmpR |
---|---|
Synonyms |
DNA-binding response regulator in two-component regulatory system with EnvZ[1], B3405[2][1], Cry[2][1], OmpB[2][1], OmpR[2][1], response regulator (sensor, EnvZ) affecting transcription of ompC and ompF: outer membrane protein synthesis[2][1], transcriptional regulatory protein OmpR[2][1], unphosphorylated transcriptional regulatory protein OmpR[2][1], unmodified transcriptional regulatory protein OmpR[2][1], Kmt[2][1] , ECK3392, JW3368, kmt, ompB, b3405 |
Product description |
Component of OmpR transcriptional dual regulator[2][3] Response regulator for osmoregulation; regulates production of outer membrane proteins[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0045893 |
positive regulation of transcription, DNA-dependent |
PMID:2558046 |
IDA: Inferred from Direct Assay |
P |
ompF promoter- Figure 4 replaces obsolete GO:0016563 |
complete | ||
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0902 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0238 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000160 |
two-component signal transduction system (phosphorelay) |
PMID:2558046 |
IDA: Inferred from Direct Assay |
P |
with CheA- Figure 4 |
complete | ||
GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0963 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0000156 |
two-component response regulator activity |
PMID:2558046 |
IDA: Inferred from Direct Assay |
F |
Figure 4 |
complete | ||
GO:0005737 |
cytoplasm |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0804 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001789 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006355 |
regulation of transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001867 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of OmpR transcriptional dual regulator |
could be indirect |
||
Protein |
prs |
PMID:16606699 |
Experiment(s):EBI-1145810 | |
Protein |
nagZ |
PMID:16606699 |
Experiment(s):EBI-1145810 | |
Protein |
cbl |
PMID:16606699 |
Experiment(s):EBI-1145810 | |
Protein |
EnvZ |
functional interaction between EnvZ and OmpR |
PMID:3536870 |
|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
cytoplasm |
From EcoCyc[3] |
| ||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MQENYKILVV DDDMRLRALL ERYLTEQGFQ VRSVANAEQM DRLLTRESFH LMVLDLMLPG EDGLSICRRL RSQSNPMPII MVTAKGEEVD RIVGLEIGAD DYIPKPFNPR ELLARIRAVL RRQANELPGA PSQEEAVIAF GKFKLNLGTR EMFREDEPMP LTSGEFAVLK ALVSHPREPL SRDKLMNLAR GREYSAMERS IDVQISRLRR MVEEDPAHPR YIQTVWGLGY VFVPDGSKA |
Length |
239 |
Mol. Wt |
27.353 kDa |
pI |
6.2 (calculated) |
Extinction coefficient |
14,440 - 14,565 (calc based on 6 Y, 1 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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<motif_map/> |
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0011110 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000665 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0666 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
6.13E+02 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
Ecoli K-12 |
80.769+/-0.719 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
mRNA |
Ecoli K-12 |
0.086926287 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
Protein |
E. coli K-12 MG1655 |
2248 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
865 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
1338 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Procaine increases levels of OmpR-phosphate in the cell. [7]
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:3534586..3534626
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
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This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for ompR | |
microarray |
Summary of data for ompR from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (3534127..3534451) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
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Notes
Accessions Related to ompR Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0666 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000665 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0011110 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Arabidopsis thaliana |
|
From Inparanoid:20070104 |
Dictyostelium discoideum |
|
From Inparanoid:20070104 |
Oryza gramene |
|
From Inparanoid:20070104 |
Saccharomyces cerevisiae |
|
From Inparanoid:20070104 |
Schizosaccharomyces pombe |
|
From Inparanoid:20070104 |
Shigella flexneri |
OMPR |
From SHIGELLACYC |
E. coli O157 |
OMPR |
From ECOO157CYC |
Staphylococcus aureus |
ARLR belongs to OMPR family of response regulators | |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10672 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000665 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0666 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0011110 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Delihas, N & Forst, S (2001) MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J. Mol. Biol. 313 1-12 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
- Genes in OpenBioSystems with Promoter Fusions
- Genes with homologs in Arabidopsis thaliana
- Genes with homologs in Dictyostelium discoideum
- Genes with homologs in Oryza gramene
- Genes with homologs in Saccharomyces cerevisiae
- Genes with homologs in Schizosaccharomyces pombe
- Genes with homologs in Shigella flexneri
- Genes with homologs in E. coli O157