ompF:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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Standard Name |
ompF |
---|---|
Gene Synonym(s) |
ECK0920, b0929, JW0912, cmlB, coa, cry, tolF, nfxB?[1][2], nfxB? |
Product Desc. |
outer membrane porin OmpF[2]; OmpF[3]; Component of outer membrane porin F[3]; The Colicin A Import System[3] Outer membrane porin F[4] |
Product Synonyms(s) |
outer membrane porin 1a (Ia;b;F)[1], B0929[2][1], TolF[2][1], Cry[2][1], Coa[2][1], CmlB[2][1], OmpF[2][1], outer membrane porin OmpF[3][1] , cmlB, coa, cry, ECK0920, JW0912, nfxB?, tolF, b0929 |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links | |
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Notes
Trimeric. T2 receptor. Colicin N receptor and translocator. Translation of OmpF is inhibited by the rseX sRNA. Binds TrxA (Kumar, 2004).[4]
Reduced levels of OmpF give multiple antibiotic resistance [5]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
ompF |
---|---|
Mnemonic |
Outer membrane protein |
Synonyms |
ECK0920, b0929, JW0912, cmlB, coa, cry, tolF, nfxB?[1][2], nfxB? |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
21.23 minutes |
MG1655: 986205..985117 |
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NC_012967: 1004074..1002986 |
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NC_012759: 888085..889173 |
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W3110 |
|
W3110: 987404..986316 |
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DH10B: 1040133..1039045 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
985120 |
Edman degradation |
PMID:7001461 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
ompF(del) (Keio:JW0912) |
deletion |
deletion |
PMID:16738554 |
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ompF627(T2R) |
T2 resistant |
PMID:3286621 |
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ompF626 |
|||||||
ompF254 |
|||||||
ompF625 |
|||||||
ompF746(del)::kan |
PMID:16738554 |
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ompFp9 |
protein expression |
OmpF is constitutively expressed in ompFp9 mutants, there is less repression than in wild-type strain |
PMID:7007334 |
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ompF - |
Resistant to |
Resistance to Cloacin DF13 |
PMID:2013565 |
Strain: LG1702 |
Figure 3 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Polymyxin |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin A |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin E2 |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin E3 |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin E3 |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin K |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin K |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin L |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Colicin L-JF246 |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Chloramphenicol |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to tetracycline |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF in strain JF557 |
Resistant to |
Resistance to Oxytetracycline |
PMID:789358 |
Strain: JF557 |
TABLE 5 | ||
ompF1466 |
Resistant to |
Resistance to beta lactam ampicillin |
PMID:6760806 |
Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11) |
Mutant is characterized by lack of ompF in strain B1466. Please see table 3. | ||
ompF1466 |
Resistant to |
Resistance to beta lactam cefoxitin |
PMID:6760806 |
Strains B1466, B1449 (ompF::Tn5), B1477 (tpo-1454), pop1387('tpo-11) |
Mutant is characterized by lack of ompF in strain B1466. Please see table 3. | ||
tolF in mutant strain JF557 |
Resistant to |
Resistant to Tetracycline |
PMID:789358 |
Strain: JF557 |
Table 5 | ||
tolF in mutant strain JF557 |
Resistant to |
Resistant to Chloramphenicol |
PMID:789358 |
Strain: JF557 |
Table 5 | ||
ompF in strain JF703 |
Resistant to |
Resistance to Colicin A |
PMID:7047502 |
Strain: JF703 |
table 2 | ||
ompF- |
Resistant to |
Introduction of ompF mutation caused a partial resistance to Microcin B17. |
PMID:3543211 |
See table 4 for complete experimental results. | |||
ompF- recA- |
Resistant to |
Introduction of double mutation caused an increase in resistance to Microcin B17. |
PMID:3543211 |
See table 4 for complete experimental results. | |||
cmlB5 |
Resistant to |
Resistant to choramphenicol as measured by zones of growth inhibition. |
PMID:374348 |
Parental Strain: RE1 CGSC
|
Growth Inhibition experiments Figure 2 | ||
ompF in mutant strain CE1121 |
Resistant to |
Resistant to TuIa phage. |
PMID:375003 |
Experimental Strain: CE1121 Parental Strain: PC0479 |
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ompF627 fadL701 |
Resistant to |
Resistance to phage T2 |
PMID:3286621 |
Experimental Strain: E15
|
| ||
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Notes
Reduced levels of OmpF gives multiple antibiotic resistance. [5]
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
JW0912 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCATGAAGCGCAATATTCTGGC Primer 2:CCGAACTGGTAAACGATACCCAC | |
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 20% [7] | |
Linked marker |
est. P1 cotransduction: 54% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000664 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EchoBASE:EB0665 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003153 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
OmpF |
---|---|
Synonyms |
outer membrane porin 1a (Ia;b;F)[1], B0929[2][1], TolF[2][1], Cry[2][1], Coa[2][1], CmlB[2][1], OmpF[2][1], outer membrane porin OmpF[3][1] , cmlB, coa, cry, ECK0920, JW0912, nfxB?, tolF, b0929 |
Product description |
outer membrane porin OmpF[2]; OmpF[3]; Component of outer membrane porin F[3]; The Colicin A Import System[3] Outer membrane porin F[4] |
EC number (for enzymes) |
|
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0005215 |
transporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001702 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005215 |
transporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001897 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0005886 |
plasma membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-1003 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001702 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001897 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0813 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006811 |
ion transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0406 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009279 |
cell outer membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0998 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0009597 |
detection of virus |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0580 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0015288 |
porin activity |
PMID:8702817 |
IMP: Inferred from Mutant Phenotype |
F |
complete | |||
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001702 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR001897 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016020 |
membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0472 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0812 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0016021 |
integral to membrane |
GO_REF:0000023 |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-9909 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0046718 |
entry of virus into host cell |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0580 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0046930 |
pore complex |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0626 |
C |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of outer membrane porin F |
could be indirect |
||
Protein |
Subunits of The Colicin A Import System |
could be indirect |
||
Protein |
OmpC |
OmpC synthesis depends on ompF gene expression |
PMID:6302079 |
|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes | |
---|---|---|---|---|---|
outer membrane |
From EcoCyc[3] |
||||
Outer Membrane |
PMID:9298646 |
| |||
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
---|---|
Sequence |
MMKRNILAVI VPALLVAGTA NAAEIYNKDG NKVDLYGKAV GLHYFSKGNG ENSYGGNGDM TYARLGFKGE TQINSDLTGY GQWEYNFQGN NSEGADAQTG NKTRLAFAGL KYADVGSFDY GRNYGVVYDA LGYTDMLPEF GGDTAYSDDF FVGRVGGVAT YRNSNFFGLV DGLNFAVQYL GKNERDTARR SNGDGVGGSI SYEYEGFGIV GAYGAADRTN LQEAQPLGNG KKAEQWATGL KYDANNIYLA ANYGETRNAT PITNKFTNTS GFANKTQDVL LVAQYQFDFG LRPSIAYTKS KAKDVEGIGD VDLVNYFEVG ATYYFNKNMS TYVDYIINQI DSDNKLGVGS DDTVAVGIVY QF |
Length |
362 |
Mol. Wt |
39.333 kDa |
pI |
4.6 (calculated) |
Extinction coefficient |
54,210 (calc based on 29 Y, 2 W, and 0 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
ASAP:ABE-0003153 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
EcoCyc:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
Escherichia coli str. K-12 substr. MG1655 | ||
RegulonDB:ECK120000664 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0665 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
Protein |
E. coli K-12 MC4100 |
1.25E+03 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
Protein |
E. coli K-12 EMG2 |
1980 |
molecules/cell |
|
Quantitative protein sequencing |
Spot ID: M25 |
PMID: 9298646 |
Protein |
E. coli K-12 EMG2 |
2160 |
molecules/cell |
|
Quantitative protein sequencing |
Spot ID: M51 |
PMID: 9298646 |
Protein |
E. coli K-12 EMG2 |
17600 |
molecules/cell |
|
Quantitative protein sequencing |
Spot ID: Ml54 |
PMID: 9298646 |
Protein |
E. coli K-12 MG1655 |
88988 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
71798 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
Protein |
E. coli K-12 MG1655 |
63340 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
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Notes
Transcription and Transcriptional Regulation
<protect>
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
![]() Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
There is a pleiotropic decrease in expression of ompF with the addition of procaine. [8]
High concentrations of sucrose decreases the amount of OmpF protein and regulates ompF gene expression. [9]
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:986185..986225
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for ompF | |
microarray |
Summary of data for ompF from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
edit table |
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Notes
Accessions Related to ompF Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0665 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000664 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003153 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
OMPF |
From SHIGELLACYC |
E. coli O157 |
OMPF |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
EcoCyc:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
EcoGene:EG10671 |
Escherichia coli str. K-12 substr. MG1655 | |
RegulonDB:ECK120000664 |
Escherichia coli str. K-12 substr. MG1655 | |
EchoBASE:EB0665 |
Escherichia coli str. K-12 substr. MG1655 | |
ASAP:ABE-0003153 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Delihas, N & Forst, S (2001) MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J. Mol. Biol. 313 1-12 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Garrett, S et al. (1983) Isolation and characterization of chain-terminating nonsense mutations in a porin regulator gene, envZ. J. Bacteriol. 156 62-9 PubMed
- ↑ Ozawa, Y & Mizushima, S (1983) Regulation of outer membrane porin protein synthesis in Escherichia coli K-12: ompF regulates the expression of ompC. J. Bacteriol. 154 669-75 PubMed
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