nusG:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

nusG

Gene Synonym(s)

ECK3973, b3982, JW3945[1], JW3945

Product Desc.

transcription termination factor NusG[2][3]

Stabilizes phage lambda protein N-NusA-RNAP antitermination complex[4]

Product Synonyms(s)

transcription termination factor[1], B3982[2][1], NusG[2][1] , ECK3973, JW3945, b3982

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): secE-nusG[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Binds TrxA (Kumar, 2004).[4]

Binds Rho [5]

Required for Rho-dependent termination in vivo [6] and enhances Rho-dependent termination in vitro [7] [8] [9]

Maintains genomic integrity [10]

Essential in rac+ strains to prevent expression of toxic prophage gene kilR [11]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nusG

Mnemonic

N (phage lambda protein) utilization substance

Synonyms

ECK3973, b3982, JW3945[1], JW3945

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90 minutes 

MG1655: 4175766..4176311
<gbrowseImage> name=NC_000913:4175766..4176311 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4157354..4157899
<gbrowseImage> name=NC_012967:4157354..4157899 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4065446..4065991
<gbrowseImage> name=NC_012759:4065446..4065991 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3458938..3458393
<gbrowseImage> name=NC_007779:3458393..3458938 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4275462..4276007
<gbrowseImage> name=NC_010473:4275462..4276007 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4175769

Edman degradation

PMID:9600841
PMID:9740056


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

MDS42 nusG(del)

deletion

Sensitivity to

increases sensitivity to Bicyclomycin

PMID:21183718

see Figure 2A

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3945

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCTGAAGCTCCTAAAAAGCG

Primer 2:CCGGCTTTTTCAACCTGGCTGAA

E11C11

Kohara Phage

Genobase

PMID:3038334

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[12]

est. P1 cotransduction: 42% [13]

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[12]

est. P1 cotransduction: 51% [13]
Synonyms:thi-39::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10667

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10667

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000660

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948485

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0661

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013026

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NusG

Synonyms

transcription termination factor[1], B3982[2][1], NusG[2][1] , ECK3973, JW3945, b3982

Product description

transcription termination factor NusG[2][3]

Stabilizes phage lambda protein N-NusA-RNAP antitermination complex[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003711

transcription elongation regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001062

F

Seeded from EcoCyc (v14.0)

complete

GO:0003711

transcription elongation regulator activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006645

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006353

transcription termination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0806

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001062

P

Seeded from EcoCyc (v14.0)

complete

GO:0031564

transcription antitermination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0889

P

Seeded from EcoCyc (v14.0)

complete

GO:0032968

positive regulation of RNA elongation from RNA polymerase II promoter

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006645

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

macA

PMID:15690043

Experiment(s):EBI-886705

Protein

arnA

PMID:15690043

Experiment(s):EBI-886705

Protein

dnaJ

PMID:15690043

Experiment(s):EBI-886705

Protein

rapA

PMID:15690043

Experiment(s):EBI-886705

Protein

mreB

PMID:15690043

Experiment(s):EBI-886705, EBI-893751

Protein

pssA

PMID:15690043

Experiment(s):EBI-886705

Protein

rho

PMID:15690043

Experiment(s):EBI-886705, EBI-891277, EBI-893067

Protein

rplB

PMID:15690043

Experiment(s):EBI-886705

Protein

rplE

PMID:15690043

Experiment(s):EBI-886705

Protein

rpoA

PMID:15690043

Experiment(s):EBI-886705, EBI-879354, EBI-883271, EBI-893067

Protein

rpoB

PMID:15690043

Experiment(s):EBI-886705, EBI-879304, EBI-883170

Protein

rpoC

PMID:15690043

Experiment(s):EBI-886705, EBI-879261, EBI-883046

Protein

rpsC

PMID:15690043

Experiment(s):EBI-886705

Protein

rpsE

PMID:15690043

Experiment(s):EBI-886705, EBI-893067

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-886705, EBI-893067

Protein

secA

PMID:15690043

Experiment(s):EBI-886705

Protein

sgbH

PMID:15690043

Experiment(s):EBI-886705

Protein

yacL

PMID:15690043

Experiment(s):EBI-886705, EBI-879082, EBI-882523, EBI-893067

Protein

deaD

PMID:15690043

Experiment(s):EBI-886705

Protein

rpoA

PMID:16606699

Experiment(s):EBI-1147147

Protein

rpoC

PMID:16606699

Experiment(s):EBI-1147147

Protein

iscS

PMID:16606699

Experiment(s):EBI-1147147

Protein

rho

PMID:16606699

Experiment(s):EBI-1147147

Protein

rpoB

PMID:16606699

Experiment(s):EBI-1147147

Protein

mukB

PMID:16606699

Experiment(s):EBI-1147147

Protein

sucB

PMID:16606699

Experiment(s):EBI-1147147

Protein

ynaA

PMID:16606699

Experiment(s):EBI-1147147

Protein

wza

PMID:16606699

Experiment(s):EBI-1147147

Protein

rapA

PMID:16606699

Experiment(s):EBI-1147147

Protein

kefA

PMID:15690043

Experiment(s):EBI-893067

Protein

rplD

PMID:15690043

Experiment(s):EBI-893067

Protein

rplL

PMID:15690043

Experiment(s):EBI-893067

Protein

rplV

PMID:15690043

Experiment(s):EBI-893067

Protein

rplW

PMID:15690043

Experiment(s):EBI-893067

Protein

rpmB

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsB

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsF

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsG

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsN

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsP

PMID:15690043

Experiment(s):EBI-893067

Protein

rpsH

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.6

Protein

gapA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsP

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rho

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):42.833204

Protein

dnaJ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):22.722310

Protein

rplB

PMID:19402753

LCMS(ID Probability):93.1 MALDI(Z-score):38.553054

Protein

rpsC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):21.904713

Protein

pssA

PMID:19402753

MALDI(Z-score):20.384978

Protein

rplW

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):14.949782

Protein

rpsU

PMID:19402753

LCMS(ID Probability):99.2

Protein

kefA

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.6

Protein

deaD

PMID:19402753

MALDI(Z-score):23.328459

Protein

rapA

PMID:19402753

MALDI(Z-score):18.521811

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV EIRGGQRRKS
ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP APISDKEVDA IMNRLQQVGD
KPRPKTLFEP GEMVRVNDGP FADFNGVVEE VDYEKSRLKV SVSIFGRATP VELDFSQVEK A
Length

181

Mol. Wt

20.53 kDa

pI

6.9 (calculated)

Extinction coefficient

15,470 (calc based on 3 Y, 2 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0AFG0

Domain

130..165

PF00467 KOW motif

PMID:19920124

Domain

7..106

PF02357 Transcription termination factor nusG

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nusG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131812

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948485

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013026

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AFG0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10667

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10667

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948485

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000660

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0661

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.60E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

18635

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

3487

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

8138

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

secE-nusG

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4175746..4175786 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3982 (EcoliWiki Page)

NCBI GEO profiles for nusG

microarray

GenExpDB:b3982 (EcoliWiki Page)

Summary of data for nusG from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to nusG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10667

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0661

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3982

EcoGene

EcoGene:EG10667

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000660

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013026

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

NUSG

From SHIGELLACYC

E. coli O157

NUSG

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00467 KOW motif

Pfam (EcoliWiki Page)

PF02357 Transcription termination factor nusG

Superfamily (EcoliWiki Page)

SUPERFAMILY:50104

Superfamily (EcoliWiki Page)

SUPERFAMILY:82679

EcoCyc

EcoCyc:EG10667

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10667

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000660

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0661

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013026

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Li, J et al. (1993) Elongation factor NusG interacts with termination factor rho to regulate termination and antitermination of transcription. Genes Dev. 7 161-72 PubMed
  6. ↑ Sullivan, SL & Gottesman, ME (1992) Requirement for E. coli NusG protein in factor-dependent transcription termination. Cell 68 989-94 PubMed
  7. ↑ Nehrke, KW & Platt, T (1994) A quaternary transcription termination complex. Reciprocal stabilization by Rho factor and NusG protein. J. Mol. Biol. 243 830-9 PubMed
  8. ↑ Burns, CM & Richardson, JP (1995) NusG is required to overcome a kinetic limitation to Rho function at an intragenic terminator. Proc. Natl. Acad. Sci. U.S.A. 92 4738-42 PubMed
  9. ↑ Washburn, RS et al. (1996) The mechanism of early transcription termination by Rho026. J. Mol. Biol. 260 347-58 PubMed
  10. ↑ Washburn, RS & Gottesman, ME (2011) Transcription termination maintains chromosome integrity. Proc. Natl. Acad. Sci. U.S.A. 108 792-7 PubMed
  11. ↑ Cardinale, CJ et al. (2008) Termination factor Rho and its cofactors NusA and NusG silence foreign DNA in E. coli. Science 320 935-8 PubMed
  12. ↑ 12.0 12.1 CGSC: The Coli Genetics Stock Center
  13. ↑ 13.0 13.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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