nuoB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nuoB |
|---|---|
| Mnemonic |
NADH:ubiquinone oxidoreductase |
| Synonyms |
ECK2281, b2287, JW5875[1], JW5875 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
51.77 minutes |
MG1655: 2402635..2401973 |
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NC_012967: 2348204..2347542 |
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NC_012759: 2287778..2288440 |
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W3110 |
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W3110: 2409283..2408621 |
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DH10B: 2494400..2493738 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2401973 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔnuoB (Keio:JW5875) |
deletion |
deletion |
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nuoB::Tn5KAN-2 (FB20747) |
Insertion at nt 608 in Plus orientation |
contains pKD46 | |||||
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nuoB::Tn5KAN-2 (FB20748) |
Insertion at nt 608 in Plus orientation |
does not contain pKD46 | |||||
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire Glucuronamide |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire L-Asparagine |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire Glycyl-L-aspartate |
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|
ΔnuoB::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW5875 |
Plasmid clone |
Status:cloned as old JW Primer 1:GCCGATTATACGCTCACCCGCAT Primer 2:CCAATCTCGTCAGGTGTACGCAG | |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 65% [10] | ||
|
zfd-1::Tn10 |
Linked marker |
est. P1 cotransduction: % [10] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Leif, H et al. (1995) Isolation and characterization of the proton-translocating NADH: ubiquinone oxidoreductase from Escherichia coli. Eur. J. Biochem. 230 538-48 PubMed
- ↑ David, P et al. (2002) Interaction of purified NDH-1 from Escherichia coli with ubiquinone analogues. Biochim. Biophys. Acta 1553 268-78 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
