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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	nhaB
Gene Synonym(s)	ECK1174, b1186, JW1175^[1], JW1175
Product Desc.	NhaB sodium/proton transporter^[2]^[3] Na+/H+ antiporter 2, weakly pH-dependent; helps regulate intracellular pH and extrude lithium^[4]
Product Synonyms(s)	sodium:proton antiporter^[1], B1186^[2]^[1], NhaB^[2]^[1] , ECK1174, JW1175, b1186
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): nhaB^[2]
Regulation/Activity
Quick Links
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Notes

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NhaB, but not NhaA, is inhibited by the diuretic drugs amiloride and harmaline. IT (Ion Transporter) superfamily (Prakash, 2003). ST(3) structural class based on hydropathy profile (Lolkema, 2003).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	nhaB
Mnemonic	Na+/H+ antiporter
Synonyms	ECK1174, b1186, JW1175^[1], JW1175
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	26.56 minutes	MG1655: 1233940..1232399 <gbrowseImage> name=NC_000913:1232399..1233940 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1234867..1233326 <gbrowseImage> name=NC_012967:1233326..1234867 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1121120..1122661 <gbrowseImage> name=NC_012759:1121120..1122661 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1236294..1234753 <gbrowseImage> name=NC_007779:1234753..1236294 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1274181..1272640 <gbrowseImage> name=NC_010473:1272640..1274181 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔnhaB (Keio:JW1175)	deletion	deletion	PMID:16738554	Shigen CGSC9081^[5]
nhaB::Tn5KAN-2 (FB20264)	Insertion at nt 496 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20264	does not contain pKD46
ΔnhaB3::Cm			PMID:8093613	CGSC:78978
ΔnhaB775::kan			PMID:16738554	CGSC:102062
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1175	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGAGATCTCCTGGGGCCGCGC Primer 2:CCAAGCGTTGCTATCCAGCCCAT
2A3	Kohara Phage	Genobase	PMID:3038334
11G8	Kohara Phage	Genobase	PMID:3038334
zcf-117::Tn10	Linked marker	CAG18463 = CGSC7363^[5]	est. P1 cotransduction: 45% ^[6]
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 94% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11392	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11392	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001359	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944822	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1365	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003984	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	NhaB
Synonyms	sodium:proton antiporter^[1], B1186^[2]^[1], NhaB^[2]^[1] , ECK1174, JW1175, b1186
Product description	NhaB sodium/proton transporter^[2]^[3] Na+/H+ antiporter 2, weakly pH-dependent; helps regulate intracellular pH and extrude lithium^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0015385	sodium:hydrogen antiporter activity	PMID:1317851	IDA: Inferred from Direct Assay		F		complete
	GO:0044459	plasma membrane part	PMID:1317851	IDA: Inferred from Direct Assay		C		complete
	GO:0006814	sodium ion transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01599	P	Seeded from EcoCyc (v14.0)	complete
	GO:0010226	response to lithium ion	PMID:1317851	IGI: Inferred from Genetic Interaction	EcoliWiki:nhaA	P		complete
	GO:0006814	sodium ion transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004671	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006814	sodium ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0739	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015081	sodium ion transmembrane transporter activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01599	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015385	sodium:hydrogen antiporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004671	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004671	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031402	sodium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0915	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MEISWGRALW RNFLGQSPDW YKLALIIFLI VNPLIFLISP FVAGWLLVAE FIFTLAMALK CYPLLPGGLL AIEAVFIGMT SAEHVREEVA ANLEVLLLLM FMVAGIYFMK QLLLFIFTRL LLSIRSKMLL SLSFCVAAAF LSAFLDALTV VAVVISVAVG FYGIYHRVAS SRTEDTDLQD DSHIDKHYKV VLEQFRGFLR SLMMHAGVGT ALGGVMTMVG EPQNLIIAKA AGWHFGDFFL RMSPVTVPVL ICGLLTCLLV EKLRWFGYGE TLPEKVREVL QQFDDQSRHQ RTRQDKIRLI VQAIIGVWLV TALALHLAEV GLIGLSVIIL ATSLTGVTDE HAIGKAFTES LPFTALLTVF FSVVAVIIDQ QLFSPIIQFV LQASEHAQLS LFYIFNGLLS SISDNVFVGT IYINEAKAAM ESGAITLKQY ELLAVAINTG TNLPSVATPN GQAAFLFLLT SALAPLIRLS YGRMVWMALP YTLVLTLVGL LCVEFTLAPV TEWFMQMGWI ATL
Length	513
Mol. Wt	56.73 kDa
pI	6.4 (calculated)
Extinction coefficient	72,880 - 73,505 (calc based on 12 Y, 10 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..512	PF06450 Bacterial Na+/H+ antiporter B (NhaB)		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nhaB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129149	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944822	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003984	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AFA7	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11392	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11392	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944822	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001359	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1365	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1060	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	187	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	465	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
nhaB
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1233920..1233960 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1186 (EcoliWiki Page)	NCBI GEO profiles for nhaB
microarray	GenExpDB:b1186 (EcoliWiki Page)	Summary of data for nhaB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to nhaB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11392	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1365	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1186
EcoGene	EcoGene:EG11392	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001359	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003984	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	NHAB	From SHIGELLACYC
E. coli O157	NHAB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF06450 Bacterial Na+/H+ antiporter B (NhaB)
EcoCyc	EcoCyc:EG11392	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11392	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001359	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1365	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003984	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

nhaB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to nhaB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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