nhaB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nhaB

Mnemonic

Na+/H+ antiporter

Synonyms

ECK1174, b1186, JW1175[1], JW1175

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.56 minutes 

MG1655: 1233940..1232399
<gbrowseImage> name=NC_000913:1232399..1233940 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1234867..1233326
<gbrowseImage> name=NC_012967:1233326..1234867 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1121120..1122661
<gbrowseImage> name=NC_012759:1121120..1122661 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1236294..1234753
<gbrowseImage> name=NC_007779:1234753..1236294 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1274181..1272640
<gbrowseImage> name=NC_010473:1272640..1274181 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔnhaB (Keio:JW1175)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9081[3]

nhaB::Tn5KAN-2 (FB20264)

Insertion at nt 496 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20264

does not contain pKD46

ΔnhaB3::Cm

PMID:8093613[5]

CGSC:78978

ΔnhaB775::kan

PMID:16738554[2]

CGSC:102062


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1175

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGAGATCTCCTGGGGCCGCGC

Primer 2:CCAAGCGTTGCTATCCAGCCCAT

2A3

Kohara Phage

Genobase

PMID:3038334[7]

11G8

Kohara Phage

Genobase

PMID:3038334[7]

zcf-117::Tn10

Linked marker

CAG18463 = CGSC7363[3]

est. P1 cotransduction: 45% [8]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 94% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11392

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11392

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001359

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944822

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1365

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003984

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Pinner, E et al. (1993) Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J. Biol. Chem. 268 1729-34 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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