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Standard Name	mmuM
Gene Synonym(s)	ECK0263, b0261, JW0253, yagD^[1], yagD
Product Desc.	MMUM^[2]^[3] S-methylmethionine:(seleno)homocysteine methyltransferase; CP4-6 putative prophage remnant^[4]
Product Synonyms(s)	CP4-6 prophage; S-methylmethionine:homocysteine methyltransferase^[1], YagD^[2]^[1], B0261^[2]^[1], MmuM^[2]^[1] , ECK0263, JW0253, yagD, b0261
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mmuP^[2]
Regulation/Activity
Quick Links
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Notes

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Met regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mmuM
Mnemonic	S-methylmethionine utilization
Synonyms	ECK0263, b0261, JW0253, yagD^[1], yagD
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	5.95 minutes	MG1655: 275939..276871 <gbrowseImage> name=NC_000913:275939..276871 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 275939..276871 <gbrowseImage> name=NC_007779:275939..276871 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 250043..250975 <gbrowseImage> name=NC_010473:250043..250975 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
mmuM(del) (Keio:JW0253)	deletion	deletion	PMID:16738554	Shigen CGSC8477^[5]
yagD::Tn5KAN-I-SceI (FB20106)	Insertion at nt 739 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20106	contains pKD46
yagD::Tn5KAN-I-SceI (FB20107)	Insertion at nt 739 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20107	does not contain pKD46
mmuM778(del)::kan			PMID:16738554	CGSC:101888
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0253	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCGCAGAATAATCCGTTACG Primer 2:CCGCTTCGCGCTTTTAACGCGGC
5A5	Kohara Phage	Genobase	PMID:3038334
3C7	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: 56% ^[6]
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[5]	est. P1 cotransduction: % ^[6] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6136	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13343	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002756	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946143	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3127	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000895	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MmuM
Synonyms	CP4-6 prophage; S-methylmethionine:homocysteine methyltransferase^[1], YagD^[2]^[1], B0261^[2]^[1], MmuM^[2]^[1] , ECK0263, JW0253, yagD, b0261
Product description	MMUM^[2]^[3] S-methylmethionine:(seleno)homocysteine methyltransferase; CP4-6 putative prophage remnant^[4]
EC number (for enzymes)	2.1.1.10^[1] 2.1.1.^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008168	methyltransferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0489	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008652	cellular amino acid biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0028	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008898	homocysteine S-methyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003726	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008898	homocysteine S-methyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.1.1.10	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009086	methionine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0486	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	nadE	PMID:16606699	Experiment(s):EBI-1136026
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1136026
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSQNNPLRAL LDKQDILLLD GAMATELEAR GCNLADSLWS AKVLVENPEL IREVHLDYYR AGAQCAITAS YQATPAGFAA RGLDEAQSKA LIGKSVELAR KAREAYLAEN PQAGTLLVAG SVGPYGAYLA DGSEYRGDYH CSVEAFQAFH RPRVEALLDA GADLLACETL PNFSEIEALA ELLTAYPRAR AWFSFTLRDS EHLSDGTPLR DVVALLAGYP QVVALGINCI ALENTTAALQ HLHGLTVLPL VVYPNSGEHY DAVSKTWHHH GEHCAQLADY LPQWQAAGAR LIGGCCRTTP ADIAALKARS
Length	310
Mol. Wt	33.422 kDa
pI	5.4 (calculated)
Extinction coefficient	41,370 - 42,370 (calc based on 13 Y, 4 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	16..310	PF02574 Homocysteine S-methyltransferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mmuM taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128246	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946143	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000895	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:Q47690	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6136	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13343	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946143	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002756	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3127	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	101	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	156	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	3826	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mmuP
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:275919..275959 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0261 (EcoliWiki Page)	NCBI GEO profiles for mmuM
microarray	GenExpDB:b0261 (EcoliWiki Page)	Summary of data for mmuM from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mmuM Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6136	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3127	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0261
EcoGene	EcoGene:EG13343	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002756	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000895	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000021355 (score: 1.000; bootstrap: 100%) ENSANGP00000020769 (score: 0.369) ENSANGP00000025029 (score: 0.095)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000025540 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G63250 (score: 1.000; bootstrap: 100%) AT3G22740 (score: 1.000; bootstrap: 100%) AT3G25900 (score: 0.316)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000002916 (score: 1.000; bootstrap: 100%) ENSBTAP00000002724 (score: 0.596)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00035790 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0032726 (score: 1.000; bootstrap: 100%) FBgn0032727 (score: 0.331)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10443-PA (score: 1.000; bootstrap: 100%) GA10445-PA (score: 0.359)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000008933 (score: 1.000; bootstrap: 100%) ENSMMUP00000012956 (score: 0.672)	From Inparanoid:20070104
Oryza gramene	Q9FUM9 (score: 1.000; bootstrap: 100%) Q9FUM8 (score: 0.920) Q7XEH2 (score: 0.465) Q9FUM7 (score: 0.426) Q5ZBZ6 (score: 0.317) Q8H825 (score: 0.308) Q9FUN0 (score: 0.280)	From Inparanoid:20070104
Saccharomyces cerevisiae	YLL062C (score: 1.000; bootstrap: 98%) YPL273W (score: 0.573)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC57A77c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000128734 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00025770001 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR21091:SF4
Superfamily (EcoliWiki Page)	SUPERFAMILY:82282
Pfam (EcoliWiki Page)	PF02574 Homocysteine S-methyltransferase
EcoCyc	EcoCyc:G6136	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13343	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002756	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3127	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000895	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mmuM:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mmuM Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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