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Expression|

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Standard Name	mltA
Gene Synonym(s)	ECK2809, b2813, JW2784, waaN, mlt, ygdM, G330^[1]^[2]
Product Desc.	membrane-bound lytic murein transglycosylase A^[2]^[3] Membrane-bound lytic transglycosylase A, murein hydrolase; periplasmic OM-attached lipoprotein^[4]
Product Synonyms(s)	membrane-bound lytic murein transglycosylase A^[1], B2813^[2]^[1], YgdM^[2]^[1], Mlt^[2]^[1], MltA^[2]^[1] , ECK2809, JW2784, mlt, ygdM, b2813
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mltA^[2]
Regulation/Activity
Quick Links
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Notes

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MltA cleaves the glycosidic bonds between the N-acetylmuramic acid and N-acetylglucosamine residues in murein. Unlike Slt and MltB, MltA can use peptide-free glycan as substrate. First 20 aa are a type II signal peptide. MltA forms a complex with MrcB and MipA.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mltA
Mnemonic	Membrane-bound lytic transglycosylase A
Synonyms	ECK2809, b2813, JW2784, waaN, mlt, ygdM, G330^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	63.45 minutes, 63.45 minutes	MG1655: 2945200..2944103 <gbrowseImage> name=NC_000913:2944103..2945200 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2841797..2840700 <gbrowseImage> name=NC_012967:2840700..2841797 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2831251..2832348 <gbrowseImage> name=NC_012759:2831251..2832348 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2945834..2944737 <gbrowseImage> name=NC_007779:2944737..2945834 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3039070..3037973 <gbrowseImage> name=NC_010473:3037973..3039070 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
ΔmltA (Keio:JW2784)	deletion	deletion			PMID:16738554	Shigen CGSC10180^[5]
ΔmltA::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔmltA741::kan					PMID:16738554	CGSC:101880
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2784	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAGGACGTTGGGTAAAGTA Primer 2:CCGCCGCTAAAGACGTTACCTGC
9A12	Kohara Phage	Genobase	PMID:3038334
10B6	Kohara Phage	Genobase	PMID:3038334
fucP3072::Tn10	Linked marker	CAG12079 = CGSC7427^[5]	est. P1 cotransduction: 66% ^[6] Synonyms:fuc-3072::Tn10
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[5]	est. P1 cotransduction: 80% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13085	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004020	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944964	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2894	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009228	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MltA
Synonyms	membrane-bound lytic murein transglycosylase A^[1], B2813^[2]^[1], YgdM^[2]^[1], Mlt^[2]^[1], MltA^[2]^[1] , ECK2809, JW2784, mlt, ygdM, b2813
Product description	membrane-bound lytic murein transglycosylase A^[2]^[3] Membrane-bound lytic transglycosylase A, murein hydrolase; periplasmic OM-attached lipoprotein^[4]
EC number (for enzymes)	3.2.1.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005300	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010611	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005887	integral to plasma membrane	PMID:8288527	IDA: Inferred from Direct Assay		C		complete
	GO:0009254	peptidoglycan turnover	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010611	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008933	lytic transglycosylase activity	PMID:8288527	IDA: Inferred from Direct Assay		F		complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016837	carbon-oxygen lyase activity, acting on polysaccharides	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019867	outer membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010611	C	Seeded from EcoCyc (v14.0)	complete
	GO:0031225	anchored to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9901	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	proV		PMID:16606699	Experiment(s):EBI-1144046
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Outer membrane		PMID:15174130	EchoLocation:mltA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKGRWVKYLL MGTVVAMLAA CSSKPTDRGQ QYKDGKFTQP FSLVNQPDAV GAPINAGDFA EQINHIRNSS PRLYGNQSNV YNAVQEWLRA GGDTRNMRQF GIDAWQMEGA DNYGNVQFTG YYTPVIQARH TRQGEFQYPI YRMPPKRGRL PSRAEIYAGA LSDKYILAYS NSLMDNFIMD VQGSGYIDFG DGSPLNFFSY AGKNGHAYRS IGKVLIDRGE VKKEDMSMQA IRHWGETHSE AEVRELLEQN PSFVFFKPQS FAPVKGASAV PLVGRASVAS DRSIIPPGTT LLAEVPLLDN NGKFNGQYEL RLMVALDVGG AIKGQHFDIY QGIGPEAGHR AGWYNHYGRV WVLKTAPGAG NVFSG
Length	365
Mol. Wt	40.41 kDa
pI	9.4 (calculated)
Extinction coefficient	61,310 - 61,435 (calc based on 19 Y, 6 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-20	UniProt Manual:Signal Peptides	UniProt:P0A935
Domain	125..257	PF03562 MltA specific insert domain		PMID:19920124
Domain	276..354	PF06725 3D domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mltA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130720	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944964	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009228	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A935	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13085	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944964	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004020	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2894	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	3.33E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	1.732+/-0.125	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.05834093	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	901	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	286	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	453	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mltA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2945180..2945220 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2813 (EcoliWiki Page)	NCBI GEO profiles for mltA
microarray	GenExpDB:b2813 (EcoliWiki Page)	Summary of data for mltA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mltA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7457	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2894	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2813
EcoGene	EcoGene:EG13085	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004020	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009228	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000001704 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MLTA	From SHIGELLACYC
E. coli O157	MLTA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03562 MltA specific insert domain
Pfam (EcoliWiki Page)	PF06725 3D domain
PFAM (EcoliWiki Page)	PFAM:PF03562
EcoCyc	EcoCyc:G7457	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13085	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004020	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2894	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009228	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mltA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mltA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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