metJ:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

metJ

Gene Synonym(s)

ECK3930, b3938, JW3909[1], JW3909

Product Desc.

MetJ transcriptional repressor[2][3]

Methionine regulon repressor, SAM co-repressor; sulfoximine and methylmethionine sensitivity[4]

Product Synonyms(s)

DNA-binding transcriptional repressor, S-adenosylmethionine-binding[1], B3938[2][1], MetJ[2][1], repressor of all met genes but metF[2][1] , ECK3930, JW3909, b3938

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): metJ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Upregulated by GSNO in defined medium (aerobic). MetJ-SAM also represses the SAM biosynthesic enzymes.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metJ

Mnemonic

Methionine

Synonyms

ECK3930, b3938, JW3909[1], JW3909

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.93 minutes 

MG1655: 4126418..4126101
<gbrowseImage> name=NC_000913:4126101..4126418 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4108502..4108185
<gbrowseImage> name=NC_012967:4108185..4108502 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4015770..4016087
<gbrowseImage> name=NC_012759:4015770..4016087 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3508286..3508603
<gbrowseImage> name=NC_007779:3508286..3508603 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4226115..4225798
<gbrowseImage> name=NC_010473:4225798..4226115 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4126104

Edman degradation

PMID:6094549


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

metJ (Keio:JW3909)

deletion

deletion

PMID:16738554

Shigen
CGSC10823[5]

metJL57Q

L57Q

(in metJ193)

Strain variation; seeded from UniProt:P0A8U6

metJA61T

A61T

(hinders dimerization)

Strain variation; seeded from UniProt:P0A8U6

metJ(del)::kan

deletion

Biolog:respiration

unable to respire b-Methyl-D-glucoside

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire a-Hydroxybutyrate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketobutyrate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire a-Ketoglutarate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire Succinate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanine

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire L-Alanyl-glycine

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938

metJ(del)::kan

deletion

Biolog:respiration

unable to respire L-Serine

PMID:16095938

metJ97(ethR)

ethionine resistant

CGSC:5734

metJ107

CGSC:9149

metJ725(del)::kan

PMID:16738554

CGSC:101838

metJ725(del)::FRTKanFRT

Mutagenesis rate

Decreased stress-induced mutagenesis (SIM) phenotype.

PMID:23224554

Parental Strain: SMR4562

Experimental Strain: SMR11997

Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population.

SMR4562 yiaG-yfp FRTcatFRT metJ725(del)::FRTKanFRT

deletion

Sigma S activity

Decrease in SigmaS activity

PMID:23224554

Parental Strain: SMR10582 Experimental Strain: SMR14269

See table S8 for full experimental results.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3909

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTGAATGGAGCGGCGAATA

Primer 2:CCGTATTCCCACGTCTCCGGGTT

12E3

Kohara Phage

Genobase

PMID:3038334

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 4% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 54% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10588

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10588

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000581

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948435

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0583

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012874

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MetJ

Synonyms

DNA-binding transcriptional repressor, S-adenosylmethionine-binding[1], B3938[2][1], MetJ[2][1], repressor of all met genes but metF[2][1] , ECK3930, JW3909, b3938

Product description

MetJ transcriptional repressor[2][3]

Methionine regulon repressor, SAM co-repressor; sulfoximine and methylmethionine sensitivity[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002084

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00744

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002084

P

Seeded from EcoCyc (v14.0)

complete

GO:0006555

methionine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002084

P

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0028

P

Seeded from EcoCyc (v14.0)

complete

GO:0009086

methionine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00744

P

Seeded from EcoCyc (v14.0)

complete

GO:0009086

methionine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0486

P

Seeded from EcoCyc (v14.0)

complete

GO:0016564

transcription repressor activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00744

F

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010985

P

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc (v14.0)

complete

GO:0045892

negative regulation of transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00744

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAEWSGEYIS PYAEHGKKSE QVKKITVSIP LKVLKILTDE RTRRQVNNLR HATNSELLCE
AFLHAFTGQP LPDDADLRKE RSDEIPEAAK EIMREMGINP ETWEY
Length

105

Mol. Wt

12.14 kDa

pI

5.4 (calculated)

Extinction coefficient

15,470 - 15,595 (calc based on 3 Y, 2 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A8U6

Domain

2..105

PF01340 Met Apo-repressor, MetJ

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=metJ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131776

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948435

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012874

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8U6

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10588

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10588

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948435

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000581

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0583

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.53E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

96.378+/-0.992

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.17259+/-0.01371

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.129337539

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

6612

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2317

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

12510

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

metJ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4126398..4126438 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3938 (EcoliWiki Page)

NCBI GEO profiles for metJ

microarray

GenExpDB:b3938 (EcoliWiki Page)

Summary of data for metJ from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4125675..4126058) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ01; Well:D9[7]

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Notes

Accessions Related to metJ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10588

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0583

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3938

EcoGene

EcoGene:EG10588

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000581

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012874

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

METJ

From SHIGELLACYC

E. coli O157

METJ

From ECOO157CYC

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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01340 Met Apo-repressor, MetJ


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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