metJ:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
metJ |
|---|---|
| Mnemonic |
Methionine |
| Synonyms |
ECK3930, b3938, JW3909[1], JW3909 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
88.93 minutes |
MG1655: 4126418..4126101 |
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NC_012967: 4108502..4108185 |
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NC_012759: 4015770..4016087 |
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W3110 |
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W3110: 3508286..3508603 |
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DH10B: 4226115..4225798 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4126104 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
metJ (Keio:JW3909) |
deletion |
deletion |
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metJL57Q |
L57Q |
(in metJ193) |
Strain variation; seeded from UniProt:P0A8U6 | ||||
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metJA61T |
A61T |
(hinders dimerization) |
Strain variation; seeded from UniProt:P0A8U6 | ||||
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire b-Methyl-D-glucoside |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire Acetate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Hydroxybutyrate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketobutyrate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire a-Ketoglutarate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire Succinate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire Bromosuccinate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanine |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Alanyl-glycine |
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|
metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Aspartate |
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metJ(del)::kan |
deletion |
Biolog:respiration |
unable to respire L-Serine |
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metJ97(ethR) |
ethionine resistant |
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metJ107 |
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metJ725(del)::kan |
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metJ725(del)::FRTKanFRT |
Mutagenesis rate |
Decreased stress-induced mutagenesis (SIM) phenotype. |
Parental Strain: SMR4562 Experimental Strain: SMR11997 |
Mutation Rate was comparatively weak, to other strains, with decrease only happening in 33-67% of wild type population. | |||
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SMR4562 yiaG-yfp FRTcatFRT metJ725(del)::FRTKanFRT |
deletion |
Sigma S activity |
Decrease in SigmaS activity |
Parental Strain: SMR10582 Experimental Strain: SMR14269 |
See table S8 for full experimental results. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3909 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCTGAATGGAGCGGCGAATA Primer 2:CCGTATTCCCACGTCTCCGGGTT | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 4% [9] | ||
|
Linked marker |
est. P1 cotransduction: 54% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Saint-Girons, I et al. (1984) Structure and autoregulation of the metJ regulatory gene in Escherichia coli. J. Biol. Chem. 259 14282-5 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
- ↑ 6.0 6.1 Al Mamun, AA et al. (2012) Identity and function of a large gene network underlying mutagenic repair of DNA breaks. Science 338 1344-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
