manX:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

manX

Gene Synonym(s)

ECK1815, b1817, JW1806, gptB, mpt, ptsL, ptsM, ptsX[1], ptsX

Product Desc.

ManX[2][3];

Component of mannose PTS permease[2][3]

Mannose permease, EIIAB component; phosphotransferase system[4]

Product Synonyms(s)

fused mannose-specific PTS enzymes: IIA component[1], IIB component[1], B1817[2][1], Mpt[2][1], PtsX[2][1], PtsM[2][1], PtsL[2][1], GptB[2][1], ManX[2][1] , ECK1815, gptB, JW1806, mpt, ptsL, ptsX, b1817

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): manXYZ[2], OP00275, ptsX

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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HT_Cmplx16_Mem: ManX+ManY+ManZ.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

manX

Mnemonic

Mannose

Synonyms

ECK1815, b1817, JW1806, gptB, mpt, ptsL, ptsM, ptsX[1], ptsX

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

40.95 minutes 

MG1655: 1900072..1901043
<gbrowseImage> name=NC_000913:1900072..1901043 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1880627..1881598
<gbrowseImage> name=NC_012967:1880627..1881598 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1792131..1793102
<gbrowseImage> name=NC_012759:1792131..1793102 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1903762..1904733
<gbrowseImage> name=NC_007779:1903762..1904733 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1990643..1991614
<gbrowseImage> name=NC_010473:1990643..1991614 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1900075

Edman degradation

PMID:9298646
PMID:10094700


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmanX (Keio:JW1806)

deletion

deletion

PMID:16738554

Shigen
CGSC9511[5]

manX::Tn5KAN-2 (FB20460)

Insertion at nt 793 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20460

contains pKD46

manX::Tn5KAN-2 (FB20461)

Insertion at nt 793 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20461

does not contain pKD46

manX6::Tn9

PMID:9864311

CGSC:7122

ΔmanX741::kan

PMID:16738554

CGSC:101756


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1806

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACCATTGCTATTGTTATAGG

Primer 2:CCtTTATCGATTTTGCTGATCAG

12B3

Kohara Phage

Genobase

PMID:3038334

15D5

Kohara Phage

Genobase

PMID:3038334

zea-225::Tn10

Linked marker

CAG18465 = CGSC7388[5]

est. P1 cotransduction: 31% [6]
Synonyms:zdj-225::Tn10

eda-51::Tn10

Linked marker

CAG18486 = CGSC7392[5]

est. P1 cotransduction: 31% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10567

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10567

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000560

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946334

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0562

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ManX

Synonyms

fused mannose-specific PTS enzymes: IIA component[1], IIB component[1], B1817[2][1], Mpt[2][1], PtsX[2][1], PtsM[2][1], PtsL[2][1], GptB[2][1], ManX[2][1] , ECK1815, gptB, JW1806, mpt, ptsL, ptsX, b1817

Product description

ManX[2][3];

Component of mannose PTS permease[2][3]

Mannose permease, EIIAB component; phosphotransferase system[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004720

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013789

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018455

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013789

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004720

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018455

F

Seeded from EcoCyc (v14.0)

complete

GO:0008982

protein-N(PI)-phosphohistidine-sugar phosphotransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.69

F

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004701

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004720

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018455

P

Seeded from EcoCyc (v14.0)

complete

GO:0009401

phosphoenolpyruvate-dependent sugar phosphotransferase system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0598

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004701

C

Seeded from EcoCyc (v14.0)

complete

GO:0016301

kinase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0418

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013789

F

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of mannose PTS permease

could be indirect

Protein

rplV

PMID:15690043

Experiment(s):EBI-892376

Protein

rpmB

PMID:15690043

Experiment(s):EBI-892376

Protein

yafC

PMID:16606699

Experiment(s):EBI-1140922

Protein

recG

PMID:16606699

Experiment(s):EBI-1140922

Protein

pgk

PMID:16606699

Experiment(s):EBI-1140922

Protein

rpmB

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]

Cytoplasm

PMID:9298646, PMID:10094700

EchoLocation:manX


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTIAIVIGTH GWAAEQLLKT AEMLLGEQEN VGWIDFVPGE NAETLIEKYN AQLAKLDTTK
GVLFLVDTWG GSPFNAASRI VVDKEHYEVI AGVNIPMLVE TLMARDDDPS FDELVALAVE
TGREGVKALK AKPVEKAAPA PAAAAPKAAP TPAKPMGPND YMVIGLARID DRLIHGQVAT
RWTKETNVSR IIVVSDEVAA DTVRKTLLTQ VAPPGVTAHV VDVAKMIRVY NNPKYAGERV
MLLFTNPTDV ERLVEGGVKI TSVNVGGMAF RQGKTQVNNA VSVDEKDIEA FKKLNARGIE
LEVRKVSTDP KLKMMDLISK IDK
Length

323

Mol. Wt

35.046 kDa

pI

5.9 (calculated)

Extinction coefficient

29,450 (calc based on 5 Y, 4 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P69797

Modification Site

72

phosphorylation site at S72

probability greater than 75%

PMID:17938405

Domain

3..119

PF03610 PTS system fructose IIA component

PMID:19920124

Domain

163..313

PF03830 PTS system sorbose subfamily IIB component

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=manX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129771

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946334

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006054

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P69797

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10567

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10567

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946334

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0562

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

57.951+/-0.456

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.20700309

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 EMG2

220

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: Ml0-2

PMID: 9298646

Protein

E. coli K-12 MG1655

2425

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2117

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1050

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

manXYZ

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1900052..1900092 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1817 (EcoliWiki Page)

NCBI GEO profiles for manX

microarray

GenExpDB:b1817 (EcoliWiki Page)

Summary of data for manX from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (1899512..1900145) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ17; Well:D1[7]

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Notes

Accessions Related to manX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10567

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0562

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1817

EcoGene

EcoGene:EG10567

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000560

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Apis mellifera

  • ENSAPMP00000021972 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

MANX

From SHIGELLACYC

E. coli O157

MANX

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03610 PTS system fructose IIA component

Superfamily (EcoliWiki Page)

SUPERFAMILY:52728

Superfamily (EcoliWiki Page)

SUPERFAMILY:53062

Pfam (EcoliWiki Page)

PF03830 PTS system sorbose subfamily IIB component

EcoCyc

EcoCyc:EG10567

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10567

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0562

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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