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Expression|

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Standard Name	malF
Gene Synonym(s)	ECK4025, b4033, JW3993^[1], JW3993, malJ
Product Desc.	MalF^[2]^[3]; Component of maltose ABC transporter^[2]^[3]; Maltose/Maltodextrin Transport System^[3] Maltose transport complex, inner membrane-spanning subunit^[4]
Product Synonyms(s)	maltose transporter subunit^[1], membrane component of ABC superfamily^[1], B4033^[2]^[1], MalF^[2]^[1] , ECK4025, JW3993, malJ, b4033
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): malEFG^[2], OP00047, malE
Regulation/Activity
Quick Links
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Notes

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The maltose transport complex MalFGK(2) also transports maltodextrin. MalT regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	malF
Mnemonic	Maltose
Synonyms	ECK4025, b4033, JW3993^[1], JW3993, malJ
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	91.42 minutes, 91.42 minutes	MG1655: 4243098..4241554 <gbrowseImage> name=NC_000913:4241554..4243098 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4224007..4222463 <gbrowseImage> name=NC_012967:4222463..4224007 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4180399..4181943 <gbrowseImage> name=NC_012759:4180399..4181943 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4248665..4247121 <gbrowseImage> name=NC_007779:4247121..4248665 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4342794..4341250 <gbrowseImage> name=NC_010473:4341250..4342794 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
malF(del) (Keio:JW3993)	deletion	deletion			PMID:16738554	Shigen CGSC10874^[5]
malFP420A		P420A		No effect			seeded from UniProt:P02916
malFG380C,S		G380C,S		No growth on maltose			seeded from UniProt:P02916
malFE401A,C,K,L		E401A,C,K,L		Reduction of transport rate			seeded from UniProt:P02916
malFS403C,D,K,L		S403C,D,K,L		Reduction of transport rate			seeded from UniProt:P02916
malFG407A,P		G407A,P		No effect			seeded from UniProt:P02916
malFL334W		L334W		Ability to transport lactose in a saturable manner			seeded from UniProt:P02916
malFL372W		L372W		Growth on maltose but not on media containing either maltoheptaose or maltoheptaose plus maltose			seeded from UniProt:P02916
malFN376K,H		N376K,H		No growth on maltose			seeded from UniProt:P02916
malF3089::Tn10	Insertion at 4,242,342 bp in MG1655 (NC_000913)				adapted from Nichols et al.^[6]	CAG12164 = CGSC7476^[5]	Synonyms:
malF64(Oc)						CGSC:7076	ochre (UAA) mutation
malF55::Tn10						CGSC:9952
malF59::Tn5						CGSC:9960
malF3089::Tn10					PMID:2540407	CGSC:40369
malF3180::Tn10kan					PMID:2540407	CGSC:40372
malF729(del)::kan					PMID:16738554	CGSC:101732
malF(del)	deletion	deletion	Resistant to	Thio(5)maltose increase resistance.	PMID:6998971		Selection for mutant maltose transport system
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3993	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATGTCATTAAAAAGAAACA Primer 2:CCATCAAACTTCATTCGCGTGGC
7A4	Kohara Phage	Genobase	PMID:3038334
1F8	Kohara Phage	Genobase	PMID:3038334
thiC39::Tn10	Linked marker	CAG18500 = CGSC7472^[5]	est. P1 cotransduction: 12% ^[6] Synonyms:thi-39::Tn10
zje-2241::Tn10	Linked marker	CAG18427 = CGSC7481^[5]	est. P1 cotransduction: 3% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10555	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10555	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000548	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948532	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0550	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013195	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MalF
Synonyms	maltose transporter subunit^[1], membrane component of ABC superfamily^[1], B4033^[2]^[1], MalF^[2]^[1] , ECK4025, JW3993, malJ, b4033
Product description	MalF^[2]^[3]; Component of maltose ABC transporter^[2]^[3]; Maltose/Maltodextrin Transport System^[3] Maltose transport complex, inner membrane-spanning subunit^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes
Protein	Subunits of maltose ABC transporter	could be indirect
Protein	Subunits of Maltose/Maltodextrin Transport System	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized in the cytoplasm with 8 predicted transmembrane domains	Daley et al. (2005) ^[7]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDVIKKKHWW QSDALKWSVL GLLGLLVGYL VVLMYAQGEY LFAITTLILS SAGLYIFANR KAYAWRYVYP GMAGMGLFVL FPLVCTIAIA FTNYSSTNQL TFERAQEVLL DRSWQAGKTY NFGLYPAGDE WQLALSDGET GKNYLSDAFK FGGEQKLQLK ETTAQPEGER ANLRVITQNR QALSDITAIL PDGNKVMMSS LRQFSGTQPL YTLDGDGTLT NNQSGVKYRP NNQIGFYQSI TADGNWGDEK LSPGYTVTTG WKNFTRVFTD EGIQKPFLAI FVWTVVFSLI TVFLTVAVGM VLACLVQWEA LRGKAVYRVL LILPYAVPSF ISILIFKGLF NQSFGEINMM LSALFGVKPA WFSDPTTART MLIIVNTWLG YPYMMILCMG LLKAIPDDLY EASAMDGAGP FQNFFKITLP LLIKPLTPLM IASFAFNFNN FVLIQLLTNG GPDRLGTTTP AGYTDLLVNY TYRIAFEGGG GQDFGLAAAI ATLIFLLVGA LAIVNLKATR MKFD
Length	514
Mol. Wt	57.016 kDa
pI	8.6 (calculated)
Extinction coefficient	100,270 - 100,645 (calc based on 23 Y, 12 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	295..508	PF00528 Binding-protein-dependent transport system inner membrane component		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=malF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131859	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948532	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013195	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P02916	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10555	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10555	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948532	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0550	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	25	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	28	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
malEFG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4243078..4243118 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4033 (EcoliWiki Page)	NCBI GEO profiles for malF
microarray	GenExpDB:b4033 (EcoliWiki Page)	Summary of data for malF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4242583..4242830) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:B1^[8]
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Notes

Accessions Related to malF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10555	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0550	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4033
EcoGene	EcoGene:EG10555	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013195	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	MALF	From SHIGELLACYC
E. coli O157	MALF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00528 Binding-protein-dependent transport system inner membrane component
EcoCyc	EcoCyc:EG10555	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10555	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000548	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0550	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013195	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

malF:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to malF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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