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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	lpxB
Gene Synonym(s)	ECK0181, b0182, JW0177, pgsB^[1], pgsB
Product Desc.	LpxB^[2]^[3]; Component of lipid A disaccharide synthase^[3] Lipid A disaccharide synthase^[4]
Product Synonyms(s)	tetraacyldisaccharide-1-P synthase^[1], B0182^[2]^[1], PgsB^[2]^[1], LpxB^[2]^[1] , ECK0181, JW0177, pgsB, b0182
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yaeT-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE^[2], ecfK-skp-lpxD-fabZ-lpxAB-rnhB-dnaE
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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The lpxB1 mutation is E326K, the result of a change in codon 326 from GAA to AAA (C.R.H. Raetz, pers. comm., 4/03, Duke Univ.). LpxB and GlpD associate.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lpxB
Mnemonic	Lipid A expression/biosynthesis
Synonyms	ECK0181, b0182, JW0177, pgsB^[1], pgsB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	4.38 minutes	MG1655: 203348..204496 <gbrowseImage> name=NC_000913:203348..204496 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 206190..207338 <gbrowseImage> name=NC_012967:206190..207338 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 203347..204495 <gbrowseImage> name=NC_012759:203347..204495 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 203348..204496 <gbrowseImage> name=NC_007779:203348..204496 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 177452..178600 <gbrowseImage> name=NC_010473:177452..178600 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	203348	Edman degradation	PMID:3049593
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
lpxB1(ts)				temperature sensitive		CGSC:9059
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0177	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACTGAACAGCGTCCATTAAC Primer 2:CCTTGTGCTAACTCCAGAACGGC
E2B8	Kohara Phage	Genobase	PMID:3038334
6F5	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 7% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 33% ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000539	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944838	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0541	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000620	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LpxB
Synonyms	tetraacyldisaccharide-1-P synthase^[1], B0182^[2]^[1], PgsB^[2]^[1], LpxB^[2]^[1] , ECK0181, JW0177, pgsB, b0182
Product description	LpxB^[2]^[3]; Component of lipid A disaccharide synthase^[3] Lipid A disaccharide synthase^[4]
EC number (for enzymes)	2.4.1.182^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0008915	lipid-A-disaccharide synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00392	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008915	lipid-A-disaccharide synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003835	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008915	lipid-A-disaccharide synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.4.1.182	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009245	lipid A biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00392	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009245	lipid A biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003835	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009245	lipid A biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0441	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019897	extrinsic to plasma membrane	PMID:19883124	IDA: Inferred from Direct Assay		C		complete
	GO:0005543	phospholipid binding	PMID:19883124	IDA: Inferred from Direct Assay		F		complete
	GO:0008915	lipid-A-disaccharide synthase activity	PMID:6370995	IDA: Inferred from Direct Assay		F		complete
	GO:0009245	lipid A biosynthetic process	PMID:6370995	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0009245	lipid A biosynthetic process	PMID:3531165	IEP: Inferred from Expression Pattern		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of lipid A disaccharide synthase	could be indirect
Protein	tufA		PMID:15690043	Experiment(s):EBI-886035, EBI-892581
Protein	sdhA		PMID:16606699	Experiment(s):EBI-1135833
Protein	mreB		PMID:16606699	Experiment(s):EBI-1135833
Protein	dnaJ		PMID:16606699	Experiment(s):EBI-1135833
Protein	ppsA		PMID:16606699	Experiment(s):EBI-1135833
Protein	rplJ		PMID:16606699	Experiment(s):EBI-1135833
Protein	dadA		PMID:16606699	Experiment(s):EBI-1135833
Protein	glpD		PMID:16606699	Experiment(s):EBI-1135833
Protein	uspG		PMID:16606699	Experiment(s):EBI-1135833
Protein	prs		PMID:16606699	Experiment(s):EBI-1135833
Protein	groL		PMID:16606699	Experiment(s):EBI-1135833
Protein	fur		PMID:16606699	Experiment(s):EBI-1135833
Protein	uppP		PMID:15690043	Experiment(s):EBI-892581
Protein	rplM		PMID:15690043	Experiment(s):EBI-892581
Protein	rplS		PMID:15690043	Experiment(s):EBI-892581
Protein	rpsB		PMID:15690043	Experiment(s):EBI-892581
Protein	torC		PMID:15690043	Experiment(s):EBI-892581
Protein	tufB		PMID:19402753	MALDI(Z-score):19.578618
Protein	rpsD		PMID:19402753	MALDI(Z-score):30.803055
Protein	torC		PMID:19402753	LCMS(ID Probability):99.0
Protein	bacA		PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTEQRPLTIA LVAGETSGDI LGAGLIRALK EHVPNARFVG VAGPRMQAEG CEAWYEMEEL AVMGIVEVLG RLRRLLHIRA DLTKRFGELK PDVFVGIDAP DFNITLEGNL KKQGIKTIHY VSPSVWAWRQ KRVFKIGRAT DLVLAFLPFE KAFYDKYNVP CRFIGHTMAD AMPLDPDKNA ARDVLGIPHD AHCLALLPGS RGAEVEMLSA DFLKTAQLLR QTYPDLEIVV PLVNAKRREQ FERIKAEVAP DLSVHLLDGM GREAMVASDA ALLASGTAAL ECMLAKCPMV VGYRMKPFTF WLAKRLVKTD YVSLPNLLAG RELVKELLQE ECEPQKLAAA LLPLLANGKT SHAMHDTFRE LHQQIRCNAD EQAAQAVLEL AQ
Length	382
Mol. Wt	42.382 kDa
pI	7.0 (calculated)
Extinction coefficient	32,430 - 33,305 (calc based on 7 Y, 4 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	9..377	PF02684 Lipid-A-disaccharide synthetase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lpxB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128175	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944838	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000620	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P10441	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10546	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944838	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000539	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0541	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	13.028+/-0.067	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.057055749	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	398	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	126	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	221	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yaeT-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:203328..203368 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0182 (EcoliWiki Page)	NCBI GEO profiles for lpxB
microarray	GenExpDB:b0182 (EcoliWiki Page)	Summary of data for lpxB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to lpxB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10546	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0541	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0182
EcoGene	EcoGene:EG10546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000539	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000620	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000029286 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT2G04560 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q5JMX5 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000016554 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	LPXB	From SHIGELLACYC
E. coli O157	LPXB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02684 Lipid-A-disaccharide synthetase
Superfamily (EcoliWiki Page)	SUPERFAMILY:53756
EcoCyc	EcoCyc:EG10546	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10546	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000539	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0541	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000620	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

lpxB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lpxB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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