lexA:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

<protect>

Standard Name

lexA

Gene Synonym(s)

ECK4035, b4043, JW4003, exrA, tsl, umuA[1], spr, umuA

Product Desc.

LexA[2][3];

Component of LexA transcriptional repressor[2][3]

Global regulator (repressor) for SOS regulon; dimeric[4]

Product Synonyms(s)

DNA-binding transcriptional repressor of SOS regulon[1], B4043[2][1], Tsl[2][1], UmuA[2][1], ExrA[2][1], LexA[2][1] , ECK4035, exrA, JW4003, spr, tsl, umuA, b4043

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): lexA-dinF[2], OP00046, lexA

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]


LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. LexA recognition site: CTGTATATATATACAG.[4]



Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lexA

Mnemonic

Lambda excision

Synonyms

ECK4035, b4043, JW4003, exrA, tsl, umuA[1], spr, umuA

edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.71 minutes 

MG1655: 4255138..4255746
<gbrowseImage> name=NC_000913:4255138..4255746 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4236047..4236655
<gbrowseImage> name=NC_012967:4236047..4236655 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4193983..4194591
<gbrowseImage> name=NC_012759:4193983..4194591 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4260705..4261313
<gbrowseImage> name=NC_007779:4260705..4261313 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4354834..4355442
<gbrowseImage> name=NC_010473:4354834..4355442 source=DH10B preset=GeneLocation </gbrowseImage>

edit table

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
edit table

<protect></protect>

Notes

A description of the lexA gene may be found in Markham et al. (1981)[5].

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lexAG85D

G85D

(in lexA3, resistant to cleavage)

Strain variation; seeded from UniProt:P0A7C2

lexA107

CGSC:6267

lexA1(Ind-)

non-inducible SOS response

CGSC:9237

lexA41(ts)

temperature sensitive

CGSC:9601

lexA3(Ind-)

non-inducible SOS response

CGSC:9602

lexA51

CGSC:10280

lexA101

CGSC:11964

lexA102

CGSC:11979

lexA109

CGSC:11985

lexA103

CGSC:14425

lexA104

CGSC:14427

lexA105

CGSC:14429

lexA106

CGSC:14431

lexA108

CGSC:14439

lexA71::Tn5

CGSC:30813

lexA2

C461T

T154I

Sensitivity to

hypocleavable repressor leads to a sensitivity to DNA damage

PMID:3127383

spr

PMID:2834329 PMID:7038393

uninducible, not cleaved during SOS response

lexA264 (Silent SalI site )

GGT->GTT

G80V

Ind- phenotype

PMID:2834329

Formic acid was used as the mutagen

lexA261 (Silent SalI site )

GGT->GAT

G80D

Ind- phenotype

PMID:2834329

Hydroxylamine was used as the mutagen

lexA266 (Silent SalI site )

GTG->GAG

V82E

Ind- phenotype

PMID:2834329

Formic acid was used as the mutagen

lexA265 (Silent SalI site )

GTG->ATG

V82M

Ind- phenotype

PMID:2834329

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA262 (Silent SalI site )

GCT->GTT

A83V

Ind- phenotype

PMID:2834329

Hydroxylamine were used as to mutagenize the strain.

lexA267 (Silent SalI site )

GCT->ACT

A83T

Ind- phenotype

PMID:2834329

Both hydroxylamine and hydrazine were used as to mutagenize the strain.

lexA252 (Silent SalI site )

GCT->--

A83Δ

Ind- phenotype

PMID:2834329

Spontaneous mutation

lexA260 (Silent SalI site )

GCC->ACC

A84T

Ind- phenotype

PMID:2834329

Hydroxylamine was used as to mutagenize the strain.

lexA268 (Silent SalI site )

GCC->GAC

A84D

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA3 (Silent SalI site )

GGT->GAT

G85D

Ind- phenotype

PMID:2834329

Hydrazine was used as to mutagenize the strain.

lexA252 (Silent SalI site )

GAA->AAA

A95K

Ind- phenotype

PMID:2834329

Spontaneous mutation

lexA274 (Silent SalI site )

GGC->CCC

R114P

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA251 (Silent SalI site )

TCG->TTG

S119L

Ind- phenotype

PMID:2834329

This allele was produced spontaneously and using hydroxylamine.

lexA269 (Silent SalI site )

GTC->TTC

V115F

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA280 (Silent SalI site )

ATG->TTG

M120L

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA263 (Silent SalI site )

GGG->GAG

G117E

Ind- phenotype

PMID:2834329

Both formic acid and hydroxylamine were used as to mutagenize the strain.

lexA259 (Silent SalI site )

ACC->ATC

T154I

Ind- phenotype

PMID:2834329

Both hydrazine and hydroxylamine were used as to mutagenize the strain.

lexA270 (Silent SalI site )

AAG->AGG

K156R

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA272 (Silent SalI site )

AAG->ATG

K156M

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA271-281

K156N/Q184H

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA271 (Silent SalI and BglI site)

AAG->AAT

K156N

Ind- phenotype

PMID:2834329

lexA281 (Silent SalI and BstI site)

CAG->CAT

Q184H

Ind- phenotype

PMID:2834329

lexA273-282

K156T/E184V

Ind- phenotype

PMID:2834329

Formic acid was used as to mutagenize the strain.

lexA273 (Silent SalI and BglI sites)

AAG->ACG

K156T

Ind- phenotype

PMID:2834329

lexA282 (Silent SalI and BglI sites)

GAA->GTA

E170V

Ind- phenotype

PMID:2834329

lexA3 in MDS42

deletion

Sensitivity to

increases senstitivity to Bicyclomycin

PMID:21183718

fig. 2

SMR6039 lexA3(Ind-)MalB::Tn9

insertion

SOS response

Decrease in spontaneous SOS-induction

PMID:23224554

Parent Strain: SMR6039 Experimental Strain: SMR6178

The mutation caused a defect in spontaneous SOS-induction. See table S7 for a summary of experimental results.

edit table

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4003

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAAAGCGTTAACGGCCAGGCA

Primer 2:CCaAGCCAGTCGCCGTTGCGAAT

1F8

Kohara Phage

Genobase

PMID:3038334

12B4

Kohara Phage

Genobase

PMID:3038334

1E4

Kohara Phage

Genobase

PMID:3038334

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[6]

est. P1 cotransduction: 60% [7]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[6]

est. P1 cotransduction: 9% [7]

DM Strain List

Strain

There are several strains with single lexA mutations listed in Table 1.

PMID:4343824

edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10533

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10533

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000526

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948544

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0528

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013241

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LexA

Synonyms

DNA-binding transcriptional repressor of SOS regulon[1], B4043[2][1], Tsl[2][1], UmuA[2][1], ExrA[2][1], LexA[2][1] , ECK4035, exrA, JW4003, spr, tsl, umuA, b4043

Product description

LexA[2][3];

Component of LexA transcriptional repressor[2][3]

Global regulator (repressor) for SOS regulon; dimeric[4]

EC number (for enzymes)
edit table

<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006197

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004252

serine-type endopeptidase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

F

Seeded from EcoCyc (v14.0)

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006199

F

Seeded from EcoCyc (v14.0)

complete

GO:0004252

serine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006200

F

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

P

Seeded from EcoCyc (v14.0)

complete

GO:0006281

DNA repair

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0234

P

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006199

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006200

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0227

P

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

P

Seeded from EcoCyc (v14.0)

complete

GO:0009432

SOS response

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0742

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006197

P

Seeded from EcoCyc (v14.0)

complete

GO:0045449

regulation of transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc (v14.0)

complete

GO:0045892

negative regulation of transcription, DNA-dependent

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00015

P

Seeded from EcoCyc (v14.0)

complete

GO:0006974

response to DNA damage stimulus

PMID:2834329

IMP: Inferred from Mutant Phenotype

P

sensitivity to MMC

complete

GO:0009432

SOS response

PMID:4343824

IMP: Inferred from Mutant Phenotype

P

complete

GO:0045892

negative regulation of transcription, DNA-dependent

PMID:10760155

IMP: Inferred from Mutant Phenotype

P

replacing obsolete GO:0016564

complete

GO:0003677

DNA binding

PMID:10760155

IDA: Inferred from Direct Assay

F

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of LexA transcriptional repressor

could be indirect

Protein

rpsE

PMID:15690043

Experiment(s):EBI-889445

Protein

skp

PMID:16606699

Experiment(s):EBI-1147325

Protein

ybiA

PMID:16606699

Experiment(s):EBI-1147325

DNA

CTGNNT(N)7CAG- consensus binding sequence

PMID:10760155

Northern blots

edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
edit table

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKALTARQQE VFDLIRDHIS QTGMPPTRAE IAQRLGFRSP NAAEEHLKAL ARKGVIEIVS
GASRGIRLLQ EEEEGLPLVG RVAAGEPLLA QQHIEGHYQV DPSLFKPNAD FLLRVSGMSM
KDIGIMDGDL LAVHKTQDVR NGQVVVARID DEVTVKRLKK QGNKVELLPE NSEFKPIVVD
LRQQSFTIEG LAVGVIRNGD WL
Length

202

Mol. Wt

22.357 kDa

pI

6.7 (calculated)

Extinction coefficient

6,990 (calc based on 1 Y, 1 W, and 0 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..65

PF01726 LexA DNA binding domain

PMID:19920124

Domain

114..182

PF00717 Peptidase S24-like

PMID:19920124

edit table

<motif_map/>

Structure

See Help:Jmol in EcoliWiki

Lex A C-terminal domain, residues 68-202 PDB:1JHE
<jmol> 

 <jmolApplet>

<color>white</color> 

   <uploadedFileContents >1JHE.pdb</uploadedFileContents>

<script> wireframe off; spacefill off; trace off; rotate ON; select *:A; spacefill on; color cyan; select *:B; cartoon on; color monomer; select *:3; color cyan; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; select all; wireframe off; spacefill off; trace off; rotate ON; select *:A; spacefill on; color cyan; select *:B; cartoon on; color monomer; select *:3; color cyan; </script><text>reset</text></jmolButton> </jmol>

See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lexA taxon=562,83333 </beststructure>

Models

View models at:

edit table
</protect>

Structure figures

<protect>

</protect>

Notes

LexA dimers bind DNA with high affinity. During the SOS response, activated RecA induces a conformational change that allows autoproteolytic cleavage of the C-terminal domain. Cleavage between A84 and G85 is catalyzed by S119, which is activated by K156 <jmol> <jmolLink> <script> rotate off; select *:B; color grey; select *:A; ribbon on; spacefill off; select 84, 85; spacefill; color yellow; select all; select 119, 156; spacefill; color cpk; select hoh; spacefill off; zoomto (84:A) 500; </script> <text>(highlight cleavage site and S119, K156A)</text> </jmolLink> </jmol>in a serine protease-like mechanism[8]. RecA-independent cleavage of LexA also occurs at high pH.

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131869

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948544

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013241

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7C2

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10533

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10533

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948544

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000526

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0528

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]




Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

2.12E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

2752

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

645

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1905

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lexA-dinF

edit table
Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<protect><gbrowseImage> name=NC_000913:4255118..4255158 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4043 (EcoliWiki Page)

NCBI GEO profiles for lexA

microarray

GenExpDB:b4043 (EcoliWiki Page)

Summary of data for lexA from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4254491..4254751) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:F11[9]

edit table

<protect></protect>

Notes

Accessions Related to lexA Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10533

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0528

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4043

EcoGene

EcoGene:EG10533

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000526

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013241

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000035850 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

LEXA

From SHIGELLACYC

E. coli O157

LEXA

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01726 LexA DNA binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:46785

Superfamily (EcoliWiki Page)

SUPERFAMILY:51306

Pfam (EcoliWiki Page)

PF00717 Peptidase S24-like

EcoCyc

EcoCyc:EG10533

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10533

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000526

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0528

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013241

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Markham, BE et al. (1981) Nucleotide sequence of the lexA gene of Escherichia coli K-12. Nucleic Acids Res. 9 4149-61 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ Roland, KL & Little, JW (1990) Reaction of LexA repressor with diisopropyl fluorophosphate. A test of the serine protease model. J. Biol. Chem. 265 12828-35 PubMed
  9. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

[back to top]


Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=lexA:On_One_Page&oldid=222434"