insN-2:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name

insN-2

Gene Synonym(s)

insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1[1][2][3]

Product Desc.

CP4-6 prophage; partial regulator of insertion element IS911A[3][2]

Product Synonyms(s)

CP4-6 prophage; partial regulator of insertion element IS911A[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.[1], B0255[3][1]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): insN[3]

Regulation/Activity
Quick Links

PortEco search(insN-2)    DNA display    Protein display    Pubmed(insN-2)    Textpresso(insN-2)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insN-2

Mnemonic
Synonyms

insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1[1][2][3], insO, ORFAB, b4587

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.81 minutes 

MG1655: 269466..269870
<gbrowseImage> name=NC_000913:269466..269870 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 269466..269870
<gbrowseImage> name=NC_007779:269466..269870 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

97.1 minutes 

MG1655: 4505220..4505486
<gbrowseImage> name=NC_000913:4505220..4505486 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4511877..4512143
<gbrowseImage> name=NC_007779:4511877..4512143 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4607028..4607294
<gbrowseImage> name=NC_010473:4607028..4607294 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

269502

Edman degradation

PMID:1660923


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

8G4

Kohara Phage

Genobase

PMID:3038334

5A5

Kohara Phage

Genobase

PMID:3038334

16H7S

Kohara Phage

Genobase

PMID:3038334

7G7

Kohara Phage

Genobase

PMID:3038334

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[4]

est. P1 cotransduction: 4% [5]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[4]

est. P1 cotransduction: % [5]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoGene (EcoliWiki Page)

EcoGene:EG40014

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:5061501

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

InsN

InsN-1

Synonyms

CP4-6 prophage; partial regulator of insertion element IS911A[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.[1], B0255[3][1] , ECK0257, insO, ORFAB, b4587

B4283[3], InsN-2[3]

Product description

CP4-6 prophage; partial regulator of insertion element IS911A[3][2]

KpLE2 phage-like element; partial regulator of insertion element IS911B (interrupted)[3][2]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology

CP4-6 prophage; partial regulator of insertion element IS911A

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002514

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004803

transposase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002514

F

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006313

transposition, DNA-mediated

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002514

P

Seeded from EcoCyc (v14.0)

complete

GO:0032196

transposition

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0815

P

Seeded from EcoCyc (v14.0)

complete


Interactions

CP4-6 prophage; partial regulator of insertion element IS911A

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MICSPQNNTG VIMKKRNFSA EFKRESAQLV VDQNYTVADA ASAMDVGLST MTRWVKQLRD
ERQGKTPKAS PITPEQIEIR ELRKKLQRIE MENEILKKAT VDSIGQRNSY VKTWGCGGFL
NETNIYSRGK SLCF

at EcoCyc

MICSPQNNTG APMKKRNFSA EFKRESAQLV VDQKYTVADA AKAMDVGLST MTRWVKQLRD
ERQGKTPKAS PITPEQIEIR KLRKKLQRIE MENEILKRLL
Length

134

100

Mol. Wt

15.249 kDa

11.558 kDa

pI

9.8 (calculated)

10.5 (calculated)

Extinction coefficient

15,470 - 15,845 (calc based on 3 Y, 2 W, and 3 C residues)

6,990 - 7,115 (calc based on 1 Y, 1 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

13..88

PF01527 Transposase

PMID:19920124

Domain

13..88

PF01527 Transposase

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insN-2 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

ASAP (EcoliWiki Page)

ASAP:ABE-0000867

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:5061501

Escherichia coli str. K-12 substr. MG1655

ASAP (EcoliWiki Page)

ASAP:ABE-0000880

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P75679

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40014

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:5061501

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

insN

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:269446..269486 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4283 (EcoliWiki Page)

NCBI GEO profiles for insN-2

microarray

GenExpDB:b4283 (EcoliWiki Page)

Summary of data for insN-2 from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4504604..269522) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ02; Well:G10[6]

GFP Fusion

Intergenic region (269333..269539) fused to gfpmut2.

OpenBioSystems

Raw protomer fusion data from Zaslaver, et al.
Plate:109-AZ14; Well:A3[6]

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Notes

Accessions Related to insN-2 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7904

EchoBASE (EcoliWiki Page)

EchoBASE:EB4745

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4283


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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

S0523

From SHIGELLACYC

E. coli O157

Z1589

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01527 Transposase

Pfam (EcoliWiki Page)

PF01527 Transposase


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. 6.0 6.1 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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