insN-2:On One Page
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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<protect> See Help:Quickview for help with entering information in the Quickview table.
Standard Name |
insN-2 |
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Gene Synonym(s) |
insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1[1][2][3] |
Product Desc. |
CP4-6 prophage; partial regulator of insertion element IS911A[3][2] |
Product Synonyms(s) |
CP4-6 prophage; partial regulator of insertion element IS911A[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.[1], B0255[3][1] |
Function from GO |
<GO_nr /> |
Knock-Out Phenotype | |
Regulation/Expression | |
Regulation/Activity | |
Quick Links |
PortEco search(insN-2) DNA display Protein display Pubmed(insN-2) Textpresso(insN-2) |
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
insN-2 |
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Mnemonic | |
Synonyms |
insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1[1][2][3], insO, ORFAB, b4587 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
---|---|---|---|---|
MG1655 |
5.81 minutes |
MG1655: 269466..269870 |
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W3110 |
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W3110: 269466..269870 |
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MG1655 |
97.1 minutes |
MG1655: 4505220..4505486 |
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W3110 |
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W3110: 4511877..4512143 |
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DH10B: 4607028..4607294 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
---|---|---|---|---|---|
Coding Start (SO:0000323) |
269502 |
Edman degradation |
PMID:1660923 |
| |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
---|---|---|---|---|---|---|---|
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
---|---|---|---|
Plasmid clone |
PMID:16769691 Status: Primer 1: Primer 2: | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Kohara Phage |
PMID:3038334 | ||
Linked marker |
est. P1 cotransduction: 4% [5] | ||
Linked marker |
est. P1 cotransduction: % [5] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
InsN |
InsN-1 |
---|---|---|
Synonyms |
CP4-6 prophage; partial regulator of insertion element IS911A[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.[1], B0255[3][1] , ECK0257, insO, ORFAB, b4587 |
|
Product description |
CP4-6 prophage; partial regulator of insertion element IS911A[3][2] |
KpLE2 phage-like element; partial regulator of insertion element IS911B (interrupted)[3][2] |
EC number (for enzymes) |
| |
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Notes
Function
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Gene Ontology
CP4-6 prophage; partial regulator of insertion element IS911A
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002514 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0238 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0004803 |
transposase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002514 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006310 |
DNA recombination |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0233 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0006313 |
transposition, DNA-mediated |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR002514 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
GO:0032196 |
transposition |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0815 |
P |
Seeded from EcoCyc (v14.0) |
complete | |
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Interactions
CP4-6 prophage; partial regulator of insertion element IS911A
See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | ||
---|---|---|
Sequence |
MICSPQNNTG VIMKKRNFSA EFKRESAQLV VDQNYTVADA ASAMDVGLST MTRWVKQLRD ERQGKTPKAS PITPEQIEIR ELRKKLQRIE MENEILKKAT VDSIGQRNSY VKTWGCGGFL NETNIYSRGK SLCF |
MICSPQNNTG APMKKRNFSA EFKRESAQLV VDQKYTVADA AKAMDVGLST MTRWVKQLRD ERQGKTPKAS PITPEQIEIR KLRKKLQRIE MENEILKRLL |
Length |
134 |
100 |
Mol. Wt |
15.249 kDa |
11.558 kDa |
pI |
9.8 (calculated) |
10.5 (calculated) |
Extinction coefficient |
15,470 - 15,845 (calc based on 3 Y, 2 W, and 3 C residues) |
6,990 - 7,115 (calc based on 1 Y, 1 W, and 1 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
---|---|---|---|---|---|---|---|
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Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
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![]() Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:269446..269486
source=MG1655
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
Allele Name | Mutation | Phenotype | Reference |
---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
Type | Reference | Notes |
---|---|---|
microarray |
NCBI GEO profiles for insN-2 | |
microarray |
Summary of data for insN-2 from multiple microarray studies | |
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Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
Resource Name | Resource Type | Description | Source | Notes |
---|---|---|---|---|
GFP Fusion |
Intergenic region (4504604..269522) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
GFP Fusion |
Intergenic region (269333..269539) fused to gfpmut2. |
Raw protomer fusion data from Zaslaver, et al. | ||
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Notes
Accessions Related to insN-2 Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
| ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
Organism | Homologs (Statistics) | Comments |
---|---|---|
Shigella flexneri |
S0523 |
From SHIGELLACYC |
E. coli O157 |
Z1589 |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
| ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 6.0 6.1 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
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