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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name	insN-2
Gene Synonym(s)	insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1^[1]^[2]^[3]
Product Desc.	CP4-6 prophage; partial regulator of insertion element IS911A^[3]^[2]
Product Synonyms(s)	CP4-6 prophage; partial regulator of insertion element IS911A^[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.^[1], B0255^[3]^[1]
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): insN^[3]
Regulation/Activity
Quick Links	PortEco search(insN-2) DNA display Protein display Pubmed(insN-2) Textpresso(insN-2)
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	insN-2
Mnemonic
Synonyms	insN, ECK0257, b0255, JW5024, yi91a, b4283, JW5771, yi91, yi91b, insN-1^[1]^[2]^[3], insO, ORFAB, b4587
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	5.81 minutes	MG1655: 269466..269870 <gbrowseImage> name=NC_000913:269466..269870 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 269466..269870 <gbrowseImage> name=NC_007779:269466..269870 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
MG1655	97.1 minutes	MG1655: 4505220..4505486 <gbrowseImage> name=NC_000913:4505220..4505486 source=ecoli preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse (MG1655)	FASTA Dump
W3110		W3110: 4511877..4512143 <gbrowseImage> name=NC_007779:4511877..4512143 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse (W3110)	FASTA Dump
DH10B		DH10B: 4607028..4607294 <gbrowseImage> name=NC_010473:4607028..4607294 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	269502	Edman degradation	PMID:1660923
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
8G4	Kohara Phage	Genobase	PMID:3038334
5A5	Kohara Phage	Genobase	PMID:3038334
16H7S	Kohara Phage	Genobase	PMID:3038334
7G7	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[4]	est. P1 cotransduction: 4% ^[5] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[4]	est. P1 cotransduction: % ^[5] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoGene (EcoliWiki Page)	EcoGene:EG40014	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:5061501	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	InsN	InsN-1
Synonyms	CP4-6 prophage; partial regulator of insertion element IS911A^[1], KpLE2 phage-like element; partial regulator of insertion element IS911B. Interrupted by insertion of IS30 element.^[1], B0255^[3]^[1] , ECK0257, insO, ORFAB, b4587	B4283^[3], InsN-2^[3]
Product description	CP4-6 prophage; partial regulator of insertion element IS911A^[3]^[2]	KpLE2 phage-like element; partial regulator of insertion element IS911B (interrupted)^[3]^[2]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology

CP4-6 prophage; partial regulator of insertion element IS911A

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002514	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004803	transposase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002514	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006313	transposition, DNA-mediated	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002514	P	Seeded from EcoCyc (v14.0)	complete
	GO:0032196	transposition	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0815	P	Seeded from EcoCyc (v14.0)	complete
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Interactions

CP4-6 prophage; partial regulator of insertion element IS911A

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MICSPQNNTG VIMKKRNFSA EFKRESAQLV VDQNYTVADA ASAMDVGLST MTRWVKQLRD ERQGKTPKAS PITPEQIEIR ELRKKLQRIE MENEILKKAT VDSIGQRNSY VKTWGCGGFL NETNIYSRGK SLCF	at EcoCyc MICSPQNNTG APMKKRNFSA EFKRESAQLV VDQKYTVADA AKAMDVGLST MTRWVKQLRD ERQGKTPKAS PITPEQIEIR KLRKKLQRIE MENEILKRLL
Length	134	100
Mol. Wt	15.249 kDa	11.558 kDa
pI	9.8 (calculated)	10.5 (calculated)
Extinction coefficient	15,470 - 15,845 (calc based on 3 Y, 2 W, and 3 C residues)	6,990 - 7,115 (calc based on 1 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	13..88	PF01527 Transposase	PMID:19920124
Domain	13..88	PF01527 Transposase	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insN-2 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
ASAP (EcoliWiki Page)	ASAP:ABE-0000867	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:5061501	Escherichia coli str. K-12 substr. MG1655
ASAP (EcoliWiki Page)	ASAP:ABE-0000880	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P75679	Escherichia coli str. K-12 substr. MG1655
EcoGene (EcoliWiki Page)	EcoGene:EG40014	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:5061501	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
insN
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:269446..269486 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4283 (EcoliWiki Page)	NCBI GEO profiles for insN-2
microarray	GenExpDB:b4283 (EcoliWiki Page)	Summary of data for insN-2 from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4504604..269522) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:G10^[6]
	GFP Fusion	Intergenic region (269333..269539) fused to gfpmut2.	OpenBioSystems	Raw protomer fusion data from Zaslaver, et al. Plate:109-AZ14; Well:A3^[6]
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Notes

Accessions Related to insN-2 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:G7904
EchoBASE (EcoliWiki Page)	EchoBASE:EB4745
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4283
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S0523	From SHIGELLACYC
E. coli O157	Z1589	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01527 Transposase
Pfam (EcoliWiki Page)	PF01527 Transposase
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 ^3.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ ^6.0 ^6.1 Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

insN-2:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

CP4-6 prophage; partial regulator of insertion element IS911A

CP4-6 prophage; partial regulator of insertion element IS911A

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to insN-2 Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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