ytfN:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ytfN

Mnemonic

Systematic nomenclature

Synonyms

ECK4217, b4221, JW4180, ytfO[1], ytfO

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

95.74 minutes 

MG1655: 4442135..4445914
<gbrowseImage> name=NC_000913:4442135..4445914 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4427869..4431648
<gbrowseImage> name=NC_012967:4427869..4431648 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4380870..4384649
<gbrowseImage> name=NC_012759:4380870..4384649 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4448792..4452571
<gbrowseImage> name=NC_007779:4448792..4452571 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4542497..4546276
<gbrowseImage> name=NC_010473:4542497..4546276 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔytfN (Keio:JW4180)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11010[3]

zjf-920::Tn10

Insertion at 4,442,823 bp in MG1655 (NC_000913)

adapted from Nichols et al.[4]

CAG12019 = CGSC7484[3]

Synonyms: zjh-901::Tn10, zjg-920::Tn10

ΔytfN770::kan

PMID:16738554[2]

CGSC:106086


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4180

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGTTTATGGAAAAAAATCAG

Primer 2:CCAAACTCGAACTGATAGAGCAA

5B5

Kohara Phage

Genobase

PMID:3038334[6]

E1H3

Kohara Phage

Genobase

PMID:3038334[6]

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[3]

est. P1 cotransduction: 57% [4]

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[3]

est. P1 cotransduction: % [4]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (EcoliWiki Page)

GeneID:948742

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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