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Expression|

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name	insL-3
Gene Synonym(s)	insL, ECK0016, b0016, JW0015, yi81-1, b0582, JW0572, yi81-2, b2394, JW5390, yi81-3, insL-1, insL-2^[1]^[2]^[3]
Product Desc.	IS186/IS421 transposase^[3]^[2]
Product Synonyms(s)	IS186/IS421 transposase^[1], predicted transposase^[1], B0582^[3]^[1], B0016^[3]^[1], InsL-1^[3]^[1]
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): insL^[3]
Regulation/Activity
Quick Links	PortEco search(insL-3) DNA display Protein display Pubmed(insL-3) Textpresso(insL-3)
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	insL-3
Mnemonic
Synonyms	insL, ECK0016, b0016, JW0015, yi81-1, b0582, JW0572, yi81-2, b2394, JW5390, yi81-3, insL-1, insL-2^[1]^[2]^[3]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	0.33 minutes	MG1655: 15445..16557 <gbrowseImage> name=NC_000913:15445..16557 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
W3110		W3110: 15445..16557 <gbrowseImage> name=NC_007779:15445..16557 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
MG1655	13.09 minutes	MG1655: 607288..608400 <gbrowseImage> name=NC_000913:607288..608400 source=ecoli preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse (MG1655)	FASTA Dump
W3110		W3110: 607288..608400 <gbrowseImage> name=NC_007779:607288..608400 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse (W3110)	FASTA Dump
MG1655	54.15 minutes	MG1655: 2512353..2513465 <gbrowseImage> name=NC_000913:2512353..2513465 source=ecoli preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse (MG1655)	FASTA Dump
W3110		W3110: 2519777..2520889 <gbrowseImage> name=NC_007779:2519777..2520889 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse (W3110)	FASTA Dump
DH10B		DH10B: 2604118..2605230 <gbrowseImage> name=NC_010473:2604118..2605230 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
insL-3(del) (Keio:)	deletion	deletion			PMID:16738554	Shigen
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID: 16769691 Status: Primer 1: Primer 2:
nupC510::Tn10	Linked marker	CAG18468 = CGSC7410^[4]	est. P1 cotransduction: 93% ^[5]
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[4]	est. P1 cotransduction: % ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	InsL	InsL-2
Synonyms	IS186/IS421 transposase^[1], predicted transposase^[1], B0582^[3]^[1], B0016^[3]^[1], InsL-1^[3]^[1]	B2394^[3], InsL-3^[3]
Product description	IS186/IS421 transposase^[3]^[2]	predicted IS186/IS421 transposase^[3]^[2]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology

IS186/IS421 transposase

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003676	nucleic acid binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012337	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004803	transposase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006313	transposition, DNA-mediated	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002559	P	Seeded from EcoCyc (v14.0)	complete
	GO:0032196	transposition	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0815	P	Seeded from EcoCyc (v14.0)	complete
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Interactions

IS186/IS421 transposase

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNYSHDNWSA ILAHIGKPEE LDTSARNAGA LTRRREIRDA ATLLRLGLAY GPGGMSLREV TAWAQLHDVA TLSDVALLKR LRNAADWFGI LAAQTLAVRA AVTGCTSGKR LRLVDGTAIS APGGGSAEWR LHMGYDPHTC QFTDFELTDS RDAERLDRFA QTADEIRIAD RGFGSRPECI RSLAFGEADY IVRVHWRGLR WLTAEGMRFD MMGFLRGLDC GKNGETTVMI GNSGNKKAGA PFPARLIAVS LPPEKALISK TRLLSENRRK GRVVQAETLE AAGHVLLLTS LPEDEYSAEQ VADCYRLRWQ IELAFKRLKS LLHLDALRAK EPELAKAWIF ANLLAAFLID DIIQPSLDFP PRSAGSEKKN	at EcoCyc MPMNYSHDNW SAILAHIGKP EELDTSARNA GALTRRREIR DAATLLRLGL AYGPGGMSLR EVTAWAQLHD VATLSDVALL KRLRNAADWF GILAAQTLAV RAAVTGCTSG KRLRLVDGTA ISAPGGGSAE WRLHMGYDPH TCQFTDFELT DSRDAERLDR FAQTADEIRI ADRGFGSRPE CIRSLAFGEA DYIVRVHWRG LRWLTAEGMR FDMMGFLRGL DCGKNGETTV MIGNSGNKKA GAPFPARLIA VSLPPEKALI SKTRLLSENR RKGRVVQAET LEAAGHVLLL TSLPEDEYSA EQVADCYRLR WQIELAFKRL KSLLHLDALR AKEPELAKAW IFANLLAAFL IDDIIQPSLD FPPRSAGSEK KN
Length	370	372
Mol. Wt	40.91 kDa	41.138 kDa
pI	8.7 (calculated)	8.7 (calculated)
Extinction coefficient	52,940 - 53,565 (calc based on 6 Y, 8 W, and 5 C residues)	52,940 - 53,565 (calc based on 6 Y, 8 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insL-3 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	11^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	116	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
insL
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:15425..15465 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to insL-3 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:G7255
EcoliGenExpDB (EcoliWiki Page)	GenExpDB:b2394
EchoBASE (EcoliWiki Page)	EchoBASE:EB4758
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 ^3.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

[LIB:Riley_2006-1] 1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)

[LIB:EcoCyc11.1-2] 2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

[LIB:EcoCyc10.6-3] 3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 ^3.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

[LIB:cgsc-4] 4.0 ^4.1 CGSC: The Coli Genetics Stock Center

[LIB:Nichols_linked_Tn-5] 5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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insL-3:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

IS186/IS421 transposase

IS186/IS421 transposase

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to insL-3 Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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