insL-3:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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<protect> See Help:Quickview for help with entering information in the Quickview table.

Standard Name

insL-3

Gene Synonym(s)

insL, ECK0016, b0016, JW0015, yi81-1, b0582, JW0572, yi81-2, b2394, JW5390, yi81-3, insL-1, insL-2[1][2][3]

Product Desc.

IS186/IS421 transposase[3][2]

Product Synonyms(s)

IS186/IS421 transposase[1], predicted transposase[1], B0582[3][1], B0016[3][1], InsL-1[3][1]

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): insL[3]

Regulation/Activity
Quick Links

PortEco search(insL-3)    DNA display    Protein display    Pubmed(insL-3)    Textpresso(insL-3)

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insL-3

Mnemonic
Synonyms

insL, ECK0016, b0016, JW0015, yi81-1, b0582, JW0572, yi81-2, b2394, JW5390, yi81-3, insL-1, insL-2[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.33 minutes 

MG1655: 15445..16557
<gbrowseImage> name=NC_000913:15445..16557 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 15445..16557
<gbrowseImage> name=NC_007779:15445..16557 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

13.09 minutes 

MG1655: 607288..608400
<gbrowseImage> name=NC_000913:607288..608400 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 607288..608400
<gbrowseImage> name=NC_007779:607288..608400 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

54.15 minutes 

MG1655: 2512353..2513465
<gbrowseImage> name=NC_000913:2512353..2513465 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2519777..2520889
<gbrowseImage> name=NC_007779:2519777..2520889 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2604118..2605230
<gbrowseImage> name=NC_010473:2604118..2605230 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

insL-3(del) (Keio:)

deletion

deletion

PMID:16738554

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID: 16769691

Status:

Primer 1:

Primer 2:

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[4]

est. P1 cotransduction: 93% [5]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: % [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

InsL

InsL-2

Synonyms

IS186/IS421 transposase[1], predicted transposase[1], B0582[3][1], B0016[3][1], InsL-1[3][1]

B2394[3], InsL-3[3]

Product description

IS186/IS421 transposase[3][2]

predicted IS186/IS421 transposase[3][2]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology

IS186/IS421 transposase

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012337

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002559

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004803

transposase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002559

F

Seeded from EcoCyc (v14.0)

complete

GO:0006310

DNA recombination

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0233

P

Seeded from EcoCyc (v14.0)

complete

GO:0006313

transposition, DNA-mediated

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002559

P

Seeded from EcoCyc (v14.0)

complete

GO:0032196

transposition

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0815

P

Seeded from EcoCyc (v14.0)

complete


Interactions

IS186/IS421 transposase

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MNYSHDNWSA ILAHIGKPEE LDTSARNAGA LTRRREIRDA ATLLRLGLAY GPGGMSLREV
TAWAQLHDVA TLSDVALLKR LRNAADWFGI LAAQTLAVRA AVTGCTSGKR LRLVDGTAIS
APGGGSAEWR LHMGYDPHTC QFTDFELTDS RDAERLDRFA QTADEIRIAD RGFGSRPECI
RSLAFGEADY IVRVHWRGLR WLTAEGMRFD MMGFLRGLDC GKNGETTVMI GNSGNKKAGA
PFPARLIAVS LPPEKALISK TRLLSENRRK GRVVQAETLE AAGHVLLLTS LPEDEYSAEQ
VADCYRLRWQ IELAFKRLKS LLHLDALRAK EPELAKAWIF ANLLAAFLID DIIQPSLDFP
PRSAGSEKKN

at EcoCyc

MPMNYSHDNW SAILAHIGKP EELDTSARNA GALTRRREIR DAATLLRLGL AYGPGGMSLR
EVTAWAQLHD VATLSDVALL KRLRNAADWF GILAAQTLAV RAAVTGCTSG KRLRLVDGTA
ISAPGGGSAE WRLHMGYDPH TCQFTDFELT DSRDAERLDR FAQTADEIRI ADRGFGSRPE
CIRSLAFGEA DYIVRVHWRG LRWLTAEGMR FDMMGFLRGL DCGKNGETTV MIGNSGNKKA
GAPFPARLIA VSLPPEKALI SKTRLLSENR RKGRVVQAET LEAAGHVLLL TSLPEDEYSA
EQVADCYRLR WQIELAFKRL KSLLHLDALR AKEPELAKAW IFANLLAAFL IDDIIQPSLD
FPPRSAGSEK KN
Length

370

372

Mol. Wt

40.91 kDa

41.138 kDa

pI

8.7 (calculated)

8.7 (calculated)

Extinction coefficient

52,940 - 53,565 (calc based on 6 Y, 8 W, and 5 C residues)

52,940 - 53,565 (calc based on 6 Y, 8 W, and 5 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=insL-3 taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

11a

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Protein

E. coli K-12 MG1655

116

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

insL

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:15425..15465 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to insL-3 Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7255

EcoliGenExpDB (EcoliWiki Page)

GenExpDB:b2394

EchoBASE (EcoliWiki Page)

EchoBASE:EB4758


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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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References

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  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).