insL-3:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insL-3

Mnemonic
Synonyms

insL, ECK0016, b0016, JW0015, yi81-1, b0582, JW0572, yi81-2, b2394, JW5390, yi81-3, insL-1, insL-2[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.33 minutes 

MG1655: 15445..16557
<gbrowseImage> name=NC_000913:15445..16557 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 15445..16557
<gbrowseImage> name=NC_007779:15445..16557 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

13.09 minutes 

MG1655: 607288..608400
<gbrowseImage> name=NC_000913:607288..608400 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 607288..608400
<gbrowseImage> name=NC_007779:607288..608400 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

54.15 minutes 

MG1655: 2512353..2513465
<gbrowseImage> name=NC_000913:2512353..2513465 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2519777..2520889
<gbrowseImage> name=NC_007779:2519777..2520889 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2604118..2605230
<gbrowseImage> name=NC_010473:2604118..2605230 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

insL-3(del) (Keio:)

deletion

deletion

PMID:16738554[4]

Shigen


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[6]

est. P1 cotransduction: 93% [7]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[6]

est. P1 cotransduction: % [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

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Notes

Links

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References

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  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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