PMID:395018
| Citation |
Shaw, KJ and Berg, CM (1979) Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5. Genetics 92:741-7 |
|---|---|
| Abstract |
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess. |
| Links | |
| Keywords |
Chromosome Mapping; Chromosomes, Bacterial; DNA Transposable Elements; Drug Resistance, Microbial; Escherichia coli/genetics; Kanamycin/pharmacology; Mutation; Neomycin/pharmacology; Recombination, Genetic |
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