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Expression|

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Standard Name	hlyE
Gene Synonym(s)	ECK1170, b1182, JW5181, clyA, sheA, ycgD, hpr^[1], hpr
Product Desc.	hemolysin E^[2]^[3] Latent secreted hemolysin E; regulated by SlyA, Fnr and HN-S^[4]
Product Synonyms(s)	hemolysin E^[1], B1182^[2]^[1], YcgD^[2]^[1], Hpr^[2]^[1], SheA^[2]^[1], ClyA^[2]^[1], HlyE^[2]^[1] , clyA, ECK1170, hpr, JW5181, sheA, ycgD, b1182
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hlyE^[2]
Regulation/Activity
Quick Links
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Notes

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HylE is secreted but has no signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hlyE
Mnemonic	Hemolysin (latent)
Synonyms	ECK1170, b1182, JW5181, clyA, sheA, ycgD, hpr^[1], hpr
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	26.48 minutes	MG1655: 1229617..1228706 <gbrowseImage> name=NC_000913:1228706..1229617 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1117427..1118338 <gbrowseImage> name=NC_012759:1117427..1118338 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1231971..1231060 <gbrowseImage> name=NC_007779:1231060..1231971 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1269858..1268947 <gbrowseImage> name=NC_010473:1268947..1269858 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1228709	Edman degradation	PMID:10027972
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔhlyE (Keio:JW5181)	deletion	deletion		PMID:16738554	Shigen CGSC11225^[5]
hlyEK175R		K175R	(in strain: CH9802)			Strain variation; seeded from UniProt:P77335
hlyEG201A		G201A	(in strain: CH9802)			Strain variation; seeded from UniProt:P77335
hlyEY97H		Y97H	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
hlyENA143DD		NA143DD	Abolishes cytotoxic activity			seeded from UniProt:P77335
hlyEN157H		N157H	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
hlyEY165C		Y165C	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
hlyE						Deletion
hlyEAG183DD		AG183DD	Abolishes cytotoxic activity			seeded from UniProt:P77335
hlyEAG187DD		AG187DD	Abolishes cytotoxic activity			seeded from UniProt:P77335
hlyER261K		R261K	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
hlyED268A		D268A	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
hlyEGVA88DVD		GVA88DVD	Abolishes cytotoxic activity			seeded from UniProt:P77335
hlyEGK293DA		GK293DA	Strongly reduces cytotoxic activity			seeded from UniProt:P77335
ΔhlyE771::kan				PMID:16738554	CGSC:101384
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5181	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACTGAAATCGTTGCAGATAA Primer 2:CCGACTTCAGGTACCTCAAAGAG
4D1	Kohara Phage	Genobase	PMID:3038334
2A3	Kohara Phage	Genobase	PMID:3038334
11G8	Kohara Phage	Genobase	PMID:3038334
zcf-117::Tn10	Linked marker	CAG18463 = CGSC7363^[5]	est. P1 cotransduction: 53% ^[6]
fadR13::Tn10	Linked marker	CAG18497 = CGSC7365^[5]	est. P1 cotransduction: 83% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6619	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13243	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003214	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945745	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HlyE
Synonyms	hemolysin E^[1], B1182^[2]^[1], YcgD^[2]^[1], Hpr^[2]^[1], SheA^[2]^[1], ClyA^[2]^[1], HlyE^[2]^[1] , clyA, ECK1170, hpr, JW5181, sheA, ycgD, b1182
Product description	hemolysin E^[2]^[3] Latent secreted hemolysin E; regulated by SlyA, Fnr and HN-S^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0009405	pathogenesis	PMID:10858262	IDA: Inferred from Direct Assay		P	Purified HlyE and E. coli cells expressing HlyE were shown to be cytotoxic to fresh or cultured human and murine monocytes/macrophages.	complete
	GO:0044532	modulation of apoptotic process in other organism	PMID:10858262	IDA: Inferred from Direct Assay		P	Purified HlyE and E. coli cells expressing HlyE were shown to be cytotoxic to fresh or cultured human and murine monocytes/macrophages.	complete
	GO:0005576	extracellular region	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0964	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005576	extracellular region	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0243	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009405	pathogenesis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0800	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9904	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019835	cytolysis	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0204	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019836	hemolysis by symbiont of host erythrocytes	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0354	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	galF		PMID:19402753	MALDI(Z-score):31.507337
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
periplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIMTEIVADK TVEVVKNAIE TADGALDLYN KYLDQVIPWQ TFDETIKELS RFKQEYSQAA SVLVGDIKTL LMDSQDKYFE ATQTVYEWCG VATQLLAAYI LLFDEYNEKK ASAQKDILIK VLDDGITKLN EAQKSLLVSS QSFNNASGKL LALDSQLTND FSEKSSYFQS QVDKIRKEAY AGAAAGVVAG PFGLIISYSI AAGVVEGKLI PELKNKLKSV QNFFTTLSNT VKQANKDIDA AKLKLTTEIA AIGEIKTETE TTRFYVDYDD LMLSLLKEAA KKMINTCNEY QKRHGKKTLF EVPEV
Length	305
Mol. Wt	34.003 kDa
pI	4.9 (calculated)
Extinction coefficient	30,370 - 30,620 (calc based on 13 Y, 2 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P77335
Domain	4..302	PF06109 Haemolysin E (HlyE)		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hlyE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:162135899	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945745	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003962	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77335	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6619	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13243	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945745	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003214	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3032	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	11	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hlyE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1229597..1229637 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1182 (EcoliWiki Page)	NCBI GEO profiles for hlyE
microarray	GenExpDB:b1182 (EcoliWiki Page)	Summary of data for hlyE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1229552..1230069) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:F4^[7]
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Notes

Accessions Related to hlyE Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6619	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3032	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1182
EcoGene	EcoGene:EG13243	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003214	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:58100
Pfam (EcoliWiki Page)	PF06109 Haemolysin E (HlyE)
EcoCyc	EcoCyc:G6619	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13243	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003214	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3032	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003962	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hlyE:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hlyE Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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