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Gene |

Product(s) |

Expression|

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Standard Name	gshA
Gene Synonym(s)	ECK2683, b2688, JW2663, gsh-I, gshI^[1], gshI
Product Desc.	gamma-Glutamylcysteine synthase^[2]
Product Synonyms(s)	gamma-glutamate-cysteine ligase^[1], B2688^[3]^[1], GshA^[3]^[1], γ-glutamylcysteine synthetase^[3]^[1], L-glutamate:L-cysteine γ-ligase (ADP-forming)^[3]^[1] , ECK2683, gshI, JW2663, b2688
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gshA^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gshA
Mnemonic	GSH, glutathione
Synonyms	ECK2683, b2688, JW2663, gsh-I, gshI^[1], gshI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	60.63 minutes, 60.63 minutes	MG1655: 2814461..2812905 <gbrowseImage> name=NC_000913:2812905..2814461 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2711298..2709742 <gbrowseImage> name=NC_012967:2709742..2711298 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2698717..2700273 <gbrowseImage> name=NC_012759:2698717..2700273 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2815095..2813539 <gbrowseImage> name=NC_007779:2813539..2815095 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2907003..2905447 <gbrowseImage> name=NC_010473:2905447..2907003 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2812905	Edman degradation	PMID:2872655
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔgshA (Keio:JW2663)	deletion	deletion	PMID:16738554	Shigen CGSC10101^[4]
gshA2				CGSC:9836
gshA1				CGSC:16015
gshA20::Tn10kan			PMID:3536846	CGSC:17037
ΔgshA769::kan			PMID:16738554	CGSC:101260
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2663	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATCCCGGACGTATCACAGGC Primer 2:CCGGCGTGTTTTTCCAGCCACAC
8G10	Kohara Phage	Genobase	PMID:3038334
23B7	Kohara Phage	Genobase	PMID:3038334
pheA18::Tn10	Linked marker	CAG12158 = CGSC7421^[4]	est. P1 cotransduction: 1% ^[5]
srlD3131::Tn10	Linked marker	CAG18642 = CGSC7423^[4]	est. P1 cotransduction: 64% ^[5] Synonyms:zfh-3131::Tn10, zfi-3131::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10418	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10418	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000411	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:944881	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0413	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008845	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GshA
Synonyms	gamma-glutamate-cysteine ligase^[1], B2688^[3]^[1], GshA^[3]^[1], γ-glutamylcysteine synthetase^[3]^[1], L-glutamate:L-cysteine γ-ligase (ADP-forming)^[3]^[1] , ECK2683, gshI, JW2663, b2688
Product description	gamma-Glutamylcysteine synthase^[2]
EC number (for enzymes)	6.3.2.2^[1]
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Notes

Structure of Gamma-Glutamylcysteine (precursor to glutathione)

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004357	glutamate-cysteine ligase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00578	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004357	glutamate-cysteine ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006334	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004357	glutamate-cysteine ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007370	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004357	glutamate-cysteine ligase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.3.2.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004357	glutamate-cysteine ligase activity	PMID:2895925	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006750	glutathione biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00578	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006750	glutathione biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006334	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006750	glutathione biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR007370	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006750	glutathione biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0317	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006750	glutathione biosynthetic process	PMID:1096956	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0006972	hyperosmotic response	PMID:1972940	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	PMID:11675389	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0071243	cellular response to arsenic	PMID:9439611	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
	GO:0071288	cellular response to mercury ion	PMID:9439611	IMP: Inferred from Mutant Phenotype		P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	mdoD	PMID:16606699	Experiment(s):EBI-1143677
Protein	ydcP	PMID:16606699	Experiment(s):EBI-1143677
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS ALTHKWITTD FAEALLEFIT PVDGDIEHML TFMRDLHRYT ARNMGDERMW PLSMPCYIAE GQDIELAQYG TSNTGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGDISGA DAKEKISAGY FRVIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTSLPFEK TECGMYYLPY ATSLRLSDLG YTNKSQSNLG ITFNDLYEYV AGLKQAIKTP SEEYAKIGIE KDGKRLQINS NVLQIENELY APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL DLFMVWCALA DAPEMSSSEL ACTRVNWNRV ILEGRKPGLT LGIGCETAQF PLPQVGKDLF RDLKRVAQTL DSINGGEAYQ KVCDELVACF DNPDLTFSAR ILRSMIDTGI GGTGKAFAEA YRNLLREEPL EILREEDFVA EREASERRQQ EMEAADTEPF AVWLEKHA
Length	518
Mol. Wt	58.271 kDa
pI	5.2 (calculated)
Extinction coefficient	75,290 - 76,415 (calc based on 21 Y, 8 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	6..381	PF04262 Glutamate-cysteine ligase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gshA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130600	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944881	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008845	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6W9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10418	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10418	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944881	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000411	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0413	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.60E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	24.934+/-0.174	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.133997429	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1300	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	502	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1197	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gshA
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2814441..2814481 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2688 (EcoliWiki Page)	NCBI GEO profiles for gshA
microarray	GenExpDB:b2688 (EcoliWiki Page)	Summary of data for gshA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2814381..2814541) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:E7^[6]
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Notes

Accessions Related to gshA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10418	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0413	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2688
EcoGene	EcoGene:EG10418	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000411	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008845	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000026609 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000024051 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	GSHA	From SHIGELLACYC
E. coli O157	GSHA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF04262 Glutamate-cysteine ligase
Superfamily (EcoliWiki Page)	SUPERFAMILY:55931
EcoCyc	EcoCyc:EG10418	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10418	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000411	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0413	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008845	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

gshA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gshA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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