gshA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GshA

Synonyms

gamma-glutamate-cysteine ligase[1], B2688[2][1], GshA[2][1], γ-glutamylcysteine synthetase[2][1], L-glutamate:L-cysteine γ-ligase (ADP-forming)[2][1] , ECK2683, gshI, JW2663, b2688

Product description

gamma-Glutamylcysteine synthase[3]

EC number (for enzymes)
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Notes

Gamma-Glutamylcysteine.jpg

Structure of Gamma-Glutamylcysteine (precursor to glutathione)

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004357

glutamate-cysteine ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00578

F

Seeded from EcoCyc (v14.0)

complete

GO:0004357

glutamate-cysteine ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006334

F

Seeded from EcoCyc (v14.0)

complete

GO:0004357

glutamate-cysteine ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007370

F

Seeded from EcoCyc (v14.0)

complete

GO:0004357

glutamate-cysteine ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.2.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0004357

glutamate-cysteine ligase activity

PMID:2895925[4]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006750

glutathione biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00578

P

Seeded from EcoCyc (v14.0)

complete

GO:0006750

glutathione biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006334

P

Seeded from EcoCyc (v14.0)

complete

GO:0006750

glutathione biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007370

P

Seeded from EcoCyc (v14.0)

complete

GO:0006750

glutathione biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0317

P

Seeded from EcoCyc (v14.0)

complete

GO:0006750

glutathione biosynthetic process

PMID:1096956[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006972

hyperosmotic response

PMID:1972940[6]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

PMID:11675389[7]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0071243

cellular response to arsenic

PMID:9439611[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0071288

cellular response to mercury ion

PMID:9439611[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

mdoD

PMID:16606699[9]

Experiment(s):EBI-1143677

Protein

ydcP

PMID:16606699[9]

Experiment(s):EBI-1143677
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIPDVSQALA WLEKHPQALK GIQRGLERET LRVNADGTLA TTGHPEALGS ALTHKWITTD
FAEALLEFIT PVDGDIEHML TFMRDLHRYT ARNMGDERMW PLSMPCYIAE GQDIELAQYG
TSNTGRFKTL YREGLKNRYG ALMQTISGVH YNFSLPMAFW QAKCGDISGA DAKEKISAGY
FRVIRNYYRF GWVIPYLFGA SPAICSSFLQ GKPTSLPFEK TECGMYYLPY ATSLRLSDLG
YTNKSQSNLG ITFNDLYEYV AGLKQAIKTP SEEYAKIGIE KDGKRLQINS NVLQIENELY
APIRPKRVTR SGESPSDALL RGGIEYIEVR SLDINPFSPI GVDEQQVRFL DLFMVWCALA
DAPEMSSSEL ACTRVNWNRV ILEGRKPGLT LGIGCETAQF PLPQVGKDLF RDLKRVAQTL
DSINGGEAYQ KVCDELVACF DNPDLTFSAR ILRSMIDTGI GGTGKAFAEA YRNLLREEPL
EILREEDFVA EREASERRQQ EMEAADTEPF AVWLEKHA
Length

518

Mol. Wt

58.271 kDa

pI

5.2 (calculated)

Extinction coefficient

75,290 - 76,415 (calc based on 21 Y, 8 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..381

PF04262 Glutamate-cysteine ligase

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gshA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130600

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944881

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008845

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6W9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10418

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10418

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944881

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000411

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0413

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. ↑ Huang, CS et al. (1988) On the active site thiol of gamma-glutamylcysteine synthetase: relationships to catalysis, inhibition, and regulation. Proc. Natl. Acad. Sci. U.S.A. 85 2464-8 PubMed
  5. ↑ Apontoweil, P & Berends, W (1975) Isolation and initial characterization of glutathione-deficient mutants of Escherichia coli K 12. Biochim. Biophys. Acta 399 10-22 PubMed
  6. ↑ McLaggan, D et al. (1990) Involvement of gamma-glutamyl peptides in osmoadaptation of Escherichia coli. J. Bacteriol. 172 3631-6 PubMed
  7. ↑ Kelly, BS et al. (2002) Escherichia coli gamma-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding. J. Biol. Chem. 277 50-8 PubMed
  8. ↑ 8.0 8.1 Latinwo, LM et al. (1998) Effects of intracellular glutathione on sensitivity of Escherichia coli to mercury and arsenite. Biochem. Biophys. Res. Commun. 242 67-70 PubMed
  9. ↑ 9.0 9.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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