gshA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gshA

Mnemonic

GSH, glutathione

Synonyms

ECK2683, b2688, JW2663, gsh-I, gshI[1], gshI

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

60.63 minutes, 60.63 minutes 

MG1655: 2814461..2812905
<gbrowseImage> name=NC_000913:2812905..2814461 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2711298..2709742
<gbrowseImage> name=NC_012967:2709742..2711298 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2698717..2700273
<gbrowseImage> name=NC_012759:2698717..2700273 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2815095..2813539
<gbrowseImage> name=NC_007779:2813539..2815095 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2907003..2905447
<gbrowseImage> name=NC_010473:2905447..2907003 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2812905

Edman degradation

PMID:2872655[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔgshA (Keio:JW2663)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10101[4]

gshA2

CGSC:9836

gshA1

CGSC:16015

gshA20::Tn10kan

PMID:3536846[5]

CGSC:17037

ΔgshA769::kan

PMID:16738554[3]

CGSC:101260


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2663

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCATCCCGGACGTATCACAGGC

Primer 2:CCGGCGTGTTTTTCCAGCCACAC

8G10

Kohara Phage

Genobase

PMID:3038334[7]

23B7

Kohara Phage

Genobase

PMID:3038334[7]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[4]

est. P1 cotransduction: 1% [8]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[4]

est. P1 cotransduction: 64% [8]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10418

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10418

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000411

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944881

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0413

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008845

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Watanabe, K et al. (1986) The nucleotide sequence of the gene for gamma-glutamylcysteine synthetase of Escherichia coli. Nucleic Acids Res. 14 4393-400 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Greenberg, JT & Demple, B (1986) Glutathione in Escherichia coli is dispensable for resistance to H2O2 and gamma radiation. J. Bacteriol. 168 1026-9 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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