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Product(s) |

Expression|

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Standard Name	fucI
Gene Synonym(s)	ECK2797, b2802, JW2773^[1], JW2773
Product Desc.	L-Fucose isomerase^[2]
Product Synonyms(s)	L-fucose isomerase^[1], B2802^[3]^[1], FucI^[3]^[1] , ECK2797, JW2773, b2802
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fucPIKUR^[3], OP00171, fucPIK
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fucI
Mnemonic	Fucose
Synonyms	ECK2797, b2802, JW2773^[1], JW2773
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	63.23 minutes	MG1655: 2933606..2935381 <gbrowseImage> name=NC_000913:2933606..2935381 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2830203..2831978 <gbrowseImage> name=NC_012967:2830203..2831978 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2820754..2822529 <gbrowseImage> name=NC_012759:2820754..2822529 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2934240..2936015 <gbrowseImage> name=NC_007779:2934240..2936015 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3027484..3029259 <gbrowseImage> name=NC_010473:3027484..3029259 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2933606	Edman degradation	PMID:8052131
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔfucI (Keio:JW2773)	deletion	deletion	PMID:16738554	Shigen
fucI::Tn5KAN-I-SceI (FB20937)	Insertion at nt 1410 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20937	contains pKD46
fucI::Tn5KAN-I-SceI (FB20938)	Insertion at nt 1410 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20938	does not contain pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2773	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAAAAATCAGCTTACCGAA Primer 2:CCACGCTTGTACAACGGACCGTA
8C5	Kohara Phage	Genobase	PMID:3038334
9A12	Kohara Phage	Genobase	PMID:3038334
fucP3072::Tn10	Linked marker	CAG12079 = CGSC7427^[4]	est. P1 cotransduction: 96% ^[5] Synonyms:fuc-3072::Tn10
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[4]	est. P1 cotransduction: 54% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10349	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10349	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000343	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946195	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0345	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FucI
Synonyms	L-fucose isomerase^[1], B2802^[3]^[1], FucI^[3]^[1] , ECK2797, JW2773, b2802
Product description	L-Fucose isomerase^[2]
EC number (for enzymes)	5.3.1.25^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01254	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004216	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005763	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012888	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012889	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR015888	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008736	L-fucose isomerase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.3.1.25	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005763	F	Seeded from EcoCyc (v14.0)	complete
	GO:0030145	manganese ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0464	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	yebT		PMID:16606699	Experiment(s):EBI-1143999
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKKISLPKIG IRPVIDGRRM GVRESLEEQT MNMAKATAAL LTEKLRHACG AAVECVISDT CIAGMAEAAA CEEKFSSQNV GLTITVTPCW CYGSETIDMD PTRPKAIWGF NGTERPGAVY LAAALAAHSQ KGIPAFSIYG HDVQDADDTS IPADVEEKLL RFARAGLAVA SMKGKSYLSL GGVSMGIAGS IVDHNFFESW LGMKVQAVDM TELRRRIDQK IYDEAELEMA LAWADKNFRY GEDENNKQYQ RNAEQSRAVL RESLLMAMCI RDMMQGNSKL ADIGRVEESL GYNAIAAGFQ GQRHWTDQYP NGDTAEAILN SSFDWNGVRE PFVVATENDS LNGVAMLMGH QLTGTAQVFA DVRTYWSPEA IERVTGHKLD GLAEHGIIHL INSGSAALDG SCKQRDSEGN PTMKPHWEIS QQEADACLAA TEWCPAIHEY FRGGGYSSRF LTEGGVPFTM TRVNIIKGLG PVLQIAEGWS VELPKDVHDI LNKRTNSTWP TTWFAPRLTG KGPFTDVYSV MANWGANHGV LTIGHVGADF ITLASMLRIP VCMHNVEETK VYRPSAWAAH GMDIEGQDYR ACQNYGPLYK R
Length	591
Mol. Wt	64.976 kDa
pI	5.7 (calculated)
Extinction coefficient	102,330 - 103,830 (calc based on 17 Y, 14 W, and 12 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	384..554	PF02952 L-fucose isomerase, C-terminal domain	PMID:19920124
Domain	4..174	PF07881 L-fucose isomerase, first N-terminal domain	PMID:19920124
Domain	175..355	PF07882 L-fucose isomerase, second N-terminal domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fucI taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130709	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946195	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009189	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P69922	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10349	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10349	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946195	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000343	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0345	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	81	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	26	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	27^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fucPIKUR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2933586..2933626 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2802 (EcoliWiki Page)	NCBI GEO profiles for fucI
microarray	GenExpDB:b2802 (EcoliWiki Page)	Summary of data for fucI from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fucI Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10349	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0345	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2802
EcoGene	EcoGene:EG10349	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000343	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FUCI	From SHIGELLACYC
E. coli O157	FUCI	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02952 L-fucose isomerase, C-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:50443
Superfamily (EcoliWiki Page)	SUPERFAMILY:53743
Pfam (EcoliWiki Page)	PF07881 L-fucose isomerase, first N-terminal domain
Pfam (EcoliWiki Page)	PF07882 L-fucose isomerase, second N-terminal domain
EcoCyc	EcoCyc:EG10349	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10349	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000343	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0345	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009189	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

fucI:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fucI Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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