fucI:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fucI

Mnemonic

Fucose

Synonyms

ECK2797, b2802, JW2773[1], JW2773

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.23 minutes 

MG1655: 2933606..2935381
<gbrowseImage> name=NC_000913:2933606..2935381 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2830203..2831978
<gbrowseImage> name=NC_012967:2830203..2831978 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2820754..2822529
<gbrowseImage> name=NC_012759:2820754..2822529 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2934240..2936015
<gbrowseImage> name=NC_007779:2934240..2936015 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3027484..3029259
<gbrowseImage> name=NC_010473:3027484..3029259 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2933606

Edman degradation

PMID:8052131[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔfucI (Keio:JW2773)

deletion

deletion

PMID:16738554[3]

Shigen

fucI::Tn5KAN-I-SceI (FB20937)

Insertion at nt 1410 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20937

contains pKD46

fucI::Tn5KAN-I-SceI (FB20938)

Insertion at nt 1410 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20938

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2773

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAAAAAATCAGCTTACCGAA

Primer 2:CCACGCTTGTACAACGGACCGTA

8C5

Kohara Phage

Genobase

PMID:3038334[6]

9A12

Kohara Phage

Genobase

PMID:3038334[6]

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[7]

est. P1 cotransduction: 96% [8]
Synonyms:fuc-3072::Tn10

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[7]

est. P1 cotransduction: 54% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10349

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10349

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000343

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946195

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0345

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009189

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Gunn, FJ et al. (1994) Identification of a novel sugar-H+ symport protein, FucP, for transport of L-fucose into Escherichia coli. Mol. Microbiol. 12 799-809 PubMed
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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