folA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
folA |
|---|---|
| Mnemonic |
Folate |
| Synonyms |
ECK0049, b0048, JW0047, tmrA, tmr[1] |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
1.07 minutes |
MG1655: 49823..50302 |
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NC_012967: 54018..54497 |
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NC_012759: 49823..50302 |
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W3110 |
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W3110: 49823..50302 |
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DH10B: 49823..50302 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
49823 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
folAL28R |
L28R |
(in strain: B[RT500] isozyme 2) |
Strain variation; seeded from UniProt:P0ABQ4 | ||||
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folAW30G |
W30G |
(in strain: 1810) |
Strain variation; seeded from UniProt:P0ABQ4 | ||||
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folAE154K |
E154K |
(in strain: B[MB1428]) |
Strain variation; seeded from UniProt:P0ABQ4 | ||||
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folAE154Q |
E154Q |
(in strain: 1810) |
Strain variation; seeded from UniProt:P0ABQ4 | ||||
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ΔfolA207::kan |
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ΔfolA201::kan |
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fol 50 |
Resistant to |
Resistance to Trimethoprim |
Strain: RS22 |
See figure 2A from parental strain D1 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0047 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATCAGTCTGATTGCGGCGTT Primer 2:CCCCGCCGCTCCAGAATCTCAAA | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 54% [9] | ||
|
leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 26% [9] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Bennett, CD et al. (1978) Dihydrofolate reductase: the amino acid sequence of the enzyme from a methotrexate-resistant mutant of Escherichia coli. Biochemistry 17 1328-37 PubMed
- ↑ Bennett, CD (1974) Letter: Primary structure of E. coli dihydrofolate reductase. Nature 252 511-2 PubMed
- ↑ Ahrweiler, PM & Frieden, C (1988) Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments. J. Bacteriol. 170 3301-4 PubMed
- ↑ Sheldon, R & Brenner, S (1976) Regulatory mutants of dihydrofolate reductase in Escherichia coli K12. Mol. Gen. Genet. 147 91-7 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
