PMID:785230
| Citation |
Sheldon, R and Brenner, S (1976) Regulatory mutants of dihydrofolate reductase in Escherichia coli K12. Mol. Gen. Genet. 147:91-7 |
|---|---|
| Abstract |
Trimethoprim inhibits dihydrofolate reductase. Mutations conferring trimethorpim-resistance on E coli K12 result in either an altered reductase with decreased affinity for the drug, or in 2-30 fold higher levels of the enzyme. Studies of the latter class of mutants indicate that dihydrofolate reductase is regulatdd by a diffusible molecule, and is probably under negative control. The regulatrory mutants, some of which are temperature-sensitive, act cis. |
| Links | |
| Keywords |
Drug Resistance, Microbial; Escherichia coli/drug effects; Escherichia coli/enzymology; Folic Acid Antagonists; Genotype; Methotrexate/pharmacology; Mutation; Tetrahydrofolate Dehydrogenase/metabolism; Trimethoprim/pharmacology |
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