folA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FolA

Synonyms

dihydrofolate reductase[1], B0048[2][1], Tmr[2][1], TmrA[2][1], FolA[2][1] , ECK0049, JW0047, tmrA, b0048

Product description

dihydrofolate reductase[2][3]

Dihydrofolate reductase; trimethoprim resistance[4]

EC number (for enzymes)

1.5.1.3[1]

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004146

dihydrofolate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001796

F

Seeded from EcoCyc (v14.0)

complete

GO:0004146

dihydrofolate reductase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

F

Seeded from EcoCyc (v14.0)

complete

GO:0042493

response to drug

PMID:6444603[5]

IDA: Inferred from Direct Assay

P

Trimethoprim

complete

GO:0004146

dihydrofolate reductase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.5.1.3

F

Seeded from EcoCyc (v14.0)

complete

GO:0006545

glycine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001796

P

Seeded from EcoCyc (v14.0)

complete

GO:0006545

glycine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

P

Seeded from EcoCyc (v14.0)

complete

GO:0006730

one-carbon metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0554

P

Seeded from EcoCyc (v14.0)

complete

GO:0009165

nucleotide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001796

P

Seeded from EcoCyc (v14.0)

complete

GO:0009165

nucleotide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

P

Seeded from EcoCyc (v14.0)

complete

GO:0016491

oxidoreductase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

F

Seeded from EcoCyc (v14.0)

complete

GO:0031427

response to methotrexate

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0487

P

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0817

P

Seeded from EcoCyc (v14.0)

complete

GO:0050661

NADP or NADPH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012259

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

lrp

PMID:16606699[6]

Experiment(s):EBI-1135444

Protein

sdhA

PMID:16606699[6]

Experiment(s):EBI-1135444

Protein

yeaG

PMID:16606699[6]

Experiment(s):EBI-1135444

Protein

thrS

PMID:16606699[6]

Experiment(s):EBI-1135444

Protein

mdoB

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

proS

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

rplM

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

ybhC

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

ssuD

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

hsrA

PMID:15690043[7]

Experiment(s):EBI-891656

Protein

proS

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

ybhC

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

ssuD

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

opgB

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

hsrA

PMID:19402753[8]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLNKPV IMGRHTWESI GRPLPGRKNI
ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE
GDTHFPDYEP DDWESVFSEF HDADAQNSHS YCFEILERR
Length

159

Mol. Wt

17.999 kDa

pI

4.7 (calculated)

Extinction coefficient

33,460 - 33,710 (calc based on 4 Y, 5 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

1..158

PF00186 Dihydrofolate reductase

PMID:19920124[9]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=folA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

See Help:Jmol in EcoliWiki

E. coli DHFR
<jmol> <jmolApplet> <color>white</color> <uploadedFileContents > 1DDS.pdb </uploadedFileContents> <name></name> <script> wireframe off; spacefill off; trace off; rotate ON; cartoon; color chain; </script> </jmolApplet> </jmol>
<jmol> <jmolCheckbox > <scriptWhenChecked > rotate on; </scriptWhenChecked > <scriptWhenUnchecked > rotate off; </scriptWhenUnchecked > <checked>true</checked> <text>rotate</text> </jmolCheckbox > <jmolButton><script>reset; </script><text>reset</text></jmolButton> </jmol>









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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128042

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000164

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABQ4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10326

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10326

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944790

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944790

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000320

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0322

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Rood, JI et al. (1980) Cloning of the Escherichia coli K-12 dihydrofolate reductase gene following mu-mediated transposition. Gene 8 255-65 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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