PMID:3290203
| Citation |
Ahrweiler, PM and Frieden, C (1988) Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments. J. Bacteriol. 170:3301-4 |
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| Abstract |
A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain. |
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| Keywords |
Chromosome Deletion; Cloning, Molecular; Drug Resistance, Microbial/genetics; Escherichia coli/drug effects; Escherichia coli/enzymology; Escherichia coli/genetics; Kanamycin/pharmacology; Mutation; Nucleic Acid Hybridization; Plasmids; Tetrahydrofolate Dehydrogenase/genetics; Tetrahydrofolate Dehydrogenase/isolation & purification; Thymidylate Synthase/genetics; Transduction, Genetic; Transformation, Bacterial |
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