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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	flu
Gene Synonym(s)	ECK1993, b2000, JW1982, agn43, agn, yeeQ, yzzX^[1], yzzX
Product Desc.	CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter^[2]^[3] Antigen 43, phase-variable bipartite outer membrane protein; Ag43; CP4-44 putative defective prophage; affects surface properties, piliation, colonial morphology; unstable gene; alpha and beta subunits formed by cleavage at residue 551^[4]
Product Synonyms(s)	CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter^[1], B2000^[2]^[1], Agn^[2]^[1], YeeQ^[2]^[1], YzzX^[2]^[1], Flu^[2]^[1], Ag43^[2]^[1] , agn, agn43, ECK1993, JW1982, yeeQ, yzzX, b2000
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): agn43^[2], flu
Regulation/Activity
Quick Links
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Notes

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Flu (Ag43) can be glycosylated in non-K-12 strains. Binds TrxA (Kumar, 2004).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	flu
Mnemonic	Flagella
Synonyms	ECK1993, b2000, JW1982, agn43, agn, yeeQ, yzzX^[1], yzzX
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	44.61 minutes	MG1655: 2069563..2072682 <gbrowseImage> name=NC_000913:2069563..2072682 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2999564..3002410 <gbrowseImage> name=NC_012967:2999564..3002410 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1962046..1965165 <gbrowseImage> name=NC_012759:1962046..1965165 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2073676..2076795 <gbrowseImage> name=NC_007779:2073676..2076795 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2160571..2163690 <gbrowseImage> name=NC_010473:2160571..2163690 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	2069719	Edman degradation	PMID:2661530 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
fluLNLVHTS829MNLIYNA		LNLVHTS829MNLIYNA	(in strain: ML 308- 225)			Strain variation; seeded from UniProt:P39180
fluATN303STI		ATN303STI	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluA320T		A320T	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluQGT845LGA		QGT845LGA	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluS855T		S855T	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluQ888L		Q888L	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluS1025I		S1025I	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluA815V		A815V	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluH585Y		H585Y	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluE709K		E709K	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluM721T		M721T	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluGHL751SHF		GHL751SHF	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluS803P		S803P	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluN372Q		N372Q	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluE493V		E493V	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluS497N		S497N	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluW157L		W157L	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluV188F		V188F	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluT46K		T46K	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluK2N		K2N	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
fluSL41FF		SL41FF	(in strain: ML 308-225)			Strain variation; seeded from UniProt:P39180
Δflu (Keio:JW1982)	deletion	deletion		PMID:16738554	Shigen CGSC9636^[5]
Δflu-768::kan				PMID:16738554	CGSC:100972
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1982	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACGACATCTGAATACCTG Primer 2:CCGAAGGTCACATTCAGTGTGGC
3B12	Kohara Phage	Genobase	PMID:3038334
2G4	Kohara Phage	Genobase	PMID:3038334
zed-3069::Tn10	Linked marker	CAG18451 = CGSC7395^[5]	est. P1 cotransduction: 27% ^[6]
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 62% ^[6] Synonyms:zee-3129::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12686	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003666	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946540	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2550	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006642	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Flu
Synonyms	CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter^[1], B2000^[2]^[1], Agn^[2]^[1], YeeQ^[2]^[1], YzzX^[2]^[1], Flu^[2]^[1], Ag43^[2]^[1] , agn, agn43, ECK1993, JW1982, yeeQ, yzzX, b2000
Product description	CP4-44 prophage; antigen 43 (Ag43) phase-variable biofilm formation autotransporter^[2]^[3] Antigen 43, phase-variable bipartite outer membrane protein; Ag43; CP4-44 putative defective prophage; affects surface properties, piliation, colonial morphology; unstable gene; alpha and beta subunits formed by cleavage at residue 551^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ydbH	PMID:16606699	Experiment(s):EBI-1141611
Protein	yehI	PMID:16606699	Experiment(s):EBI-1141611
Protein	gadB	PMID:19402753	LCMS(ID Probability):99.6
Protein	groS	PMID:19402753	LCMS(ID Probability):99.6
Protein	adhE	PMID:19402753	LCMS(ID Probability):99.6
Protein	gapA	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplT	PMID:19402753	LCMS(ID Probability):99.6
Protein	ahpC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplJ	PMID:19402753	LCMS(ID Probability):99.6
Protein	yfiD	PMID:19402753	LCMS(ID Probability):99.6
Protein	pflB	PMID:19402753	LCMS(ID Probability):99.6
Protein	dps	PMID:19402753	LCMS(ID Probability):99.6
Protein	eno	PMID:19402753	LCMS(ID Probability):99.6
Protein	hns	PMID:19402753	LCMS(ID Probability):99.6
Protein	wrbA	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsN	PMID:19402753	LCMS(ID Probability):99.6
Protein	pgk	PMID:19402753	LCMS(ID Probability):99.6
Protein	rho	PMID:19402753	LCMS(ID Probability):99.6
Protein	hupA	PMID:19402753	LCMS(ID Probability):99.6
Protein	mdh	PMID:19402753	LCMS(ID Probability):99.2
Protein	rplW	PMID:19402753	LCMS(ID Probability):99.6
Protein	hupB	PMID:19402753	LCMS(ID Probability):99.6
Protein	cspC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpoA	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
outer membrane
Outer Membrane	PMID:9298646	EchoLocation:flu
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKRHLNTCYR LVWNHMTGAF VVASELARAR GKRGGVAVAL SLAAVTSLPV LAADIVVHPG ETVNGGTLAN HDNQIVFGTT NGMTISTGLE YGPDNEANTG GQWVQDGGTA NKTTVTSGGL QRVNPGGSVS DTVISAGGGQ SLQGRAVNTT LNGGEQWMHE GAIATGTVIN DKGWQVVKPG TVATDTVVNT GAEGGPDAEN GDTGQFVRGD AVRTTINKNG RQIVRAEGTA NTTVVYAGGD QTVHGHALDT TLNGGYQYVH NGGTASDTVV NSDGWQIVKN GGVAGNTTVN QKGRLQVDAG GTATNVTLKQ GGALVTSTAA TVTGINRLGA FSVVEGKADN VVLENGGRLD VLTGHTATNT RVDDGGTLDV RNGGTATTVS MGNGGVLLAD SGAAVSGTRS DGKAFSIGGG QADALMLEKG SSFTLNAGDT ATDTTVNGGL FTARGGTLAG TTTLNNGAIL TLSGKTVNND TLTIREGDAL LQGGSLTGNG SVEKSGSGTL TVSNTTLTQK AVNLNEGTLT LNDSTVTTDV IAQRGTALKL TGSTVLNGAI DPTNVTLASG ATWNIPDNAT VQSVVDDLSH AGQIHFTSTR TGKFVPATLK VKNLNGQNGT ISLRVRPDMA QNNADRLVID GGRATGKTIL NLVNAGNSAS GLATSGKGIQ VVEAINGATT EEGAFVQGNR LQAGAFNYSL NRDSDESWYL RSENAYRAEV PLYASMLTQA MDYDRIVAGS RSHQTGVNGE NNSVRLSIQG GHLGHDNNGG IARGATPESS GSYGFVRLEG DLMRTEVAGM SVTAGVYGAA GHSSVDVKDD DGSRAGTVRD DAGSLGGYLN LVHTSSGLWA DIVAQGTRHS MKASSDNNDF RARGWGWLGS LETGLPFSIT DNLMLEPQLQ YTWQGLSLDD GKDNAGYVKF GHGSAQHVRA GFRLGSHNDM TFGEGTSSRA PLRDSAKHSV SELPVNWWVQ PSVIRTFSSR GDMRVGTSTA GSGMTFSPSQ NGTSLDLQAG LEARVRENIT LGVQAGYAHS VSGSSAEGYN GQATLNVTF
Length	1,039
Mol. Wt	106.822 kDa
pI	6.1 (calculated)
Extinction coefficient	96,830 - 96,955 (calc based on 17 Y, 13 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-52	UniProt Manual:Signal Peptides	UniProt:P39180
Domain	738..1023	PF03797 Autotransporter beta-domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flu taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176177	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946540	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006642	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39180	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12686	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946540	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003666	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2550	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	2.273+/-0.083	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.189804424	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	122	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	123	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	165	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
agn43
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2069543..2069583 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2000 (EcoliWiki Page)	NCBI GEO profiles for flu
microarray	GenExpDB:b2000 (EcoliWiki Page)	Summary of data for flu from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2069144..2069455) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:E10^[7]
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Notes

Accessions Related to flu Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7080	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2550	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2000
EcoGene	EcoGene:EG12686	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003666	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006642	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	S3195	From SHIGELLACYC
E. coli O157	Z1211	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03797 Autotransporter beta-domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:51126
Superfamily (EcoliWiki Page)	SUPERFAMILY:103515
PFAM (EcoliWiki Page)	PFAM:PF03212
EcoCyc	EcoCyc:G7080	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12686	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003666	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2550	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006642	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

flu:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to flu Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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