flu:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

flu

Mnemonic

Flagella

Synonyms

ECK1993, b2000, JW1982, agn43, agn, yeeQ, yzzX[1], yzzX

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

44.61 minutes 

MG1655: 2069563..2072682
<gbrowseImage> name=NC_000913:2069563..2072682 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2999564..3002410
<gbrowseImage> name=NC_012967:2999564..3002410 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1962046..1965165
<gbrowseImage> name=NC_012759:1962046..1965165 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2073676..2076795
<gbrowseImage> name=NC_007779:2073676..2076795 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2160571..2163690
<gbrowseImage> name=NC_010473:2160571..2163690 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2069719

Edman degradation

PMID:2661530[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

fluLNLVHTS829MNLIYNA

LNLVHTS829MNLIYNA

(in strain: ML 308- 225)

Strain variation; seeded from UniProt:P39180

fluATN303STI

ATN303STI

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluA320T

A320T

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluQGT845LGA

QGT845LGA

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluS855T

S855T

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluQ888L

Q888L

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluS1025I

S1025I

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluA815V

A815V

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluH585Y

H585Y

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluE709K

E709K

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluM721T

M721T

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluGHL751SHF

GHL751SHF

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluS803P

S803P

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluN372Q

N372Q

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluE493V

E493V

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluS497N

S497N

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluW157L

W157L

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluV188F

V188F

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluT46K

T46K

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluK2N

K2N

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

fluSL41FF

SL41FF

(in strain: ML 308-225)

Strain variation; seeded from UniProt:P39180

Δflu (Keio:JW1982)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9636[5]

Δflu-768::kan

PMID:16738554[4]

CGSC:100972


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1982

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAACGACATCTGAATACCTG

Primer 2:CCGAAGGTCACATTCAGTGTGGC

3B12

Kohara Phage

Genobase

PMID:3038334[7]

2G4

Kohara Phage

Genobase

PMID:3038334[7]

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[5]

est. P1 cotransduction: 27% [8]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[5]

est. P1 cotransduction: 62% [8]
Synonyms:zee-3129::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7080

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12686

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003666

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946540

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2550

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006642

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Caffrey, P & Owen, P (1989) Purification and N-terminal sequence of the alpha subunit of antigen 43, a unique protein complex associated with the outer membrane of Escherichia coli. J. Bacteriol. 171 3634-40 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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