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Product(s) |

Expression|

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Standard Name	fliK
Gene Synonym(s)	ECK1941, b1943, JW1927, flaE, flaR^[1]^[2]
Product Desc.	flagellar hook-length control protein FliK^[2]^[3]
Product Synonyms(s)	flagellar hook-length control protein^[1], B1943^[2]^[1], FlaR^[2]^[1], FlaE^[2]^[1], FliK^[2]^[1] , ECK1941, flaE, JW1927, b1943
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fliFGHIJK^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fliK
Mnemonic	Flagella
Synonyms	ECK1941, b1943, JW1927, flaE, flaR^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	43.46 minutes	MG1655: 2016410..2017537 <gbrowseImage> name=NC_000913:2016410..2017537 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 1908893..1910020 <gbrowseImage> name=NC_012759:1908893..1910020 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2020523..2021650 <gbrowseImage> name=NC_007779:2020523..2021650 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2107418..2108545 <gbrowseImage> name=NC_010473:2107418..2108545 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔfliK (Keio:JW1927)	deletion	deletion		PMID:16738554	Shigen CGSC9603^[4]
fliK::Tn5KAN-2 (FB20542)	Insertion at nt 49 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20542	contains pKD46
fliK::Tn5KAN-2 (FB20543)	Insertion at nt 49 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20543	does not contain pKD46
ΔfliK789::kan				PMID:16738554	CGSC:100946
fliK(S319Y)		S319Y	Severely affects motility.	PMID:16949608
fliK(R336G)		R336G	Mildly affects motility.	PMID:16949608
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1927	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTCGCTTAGCGCCCTTGAT Primer 2:CCGGCGAAAATATCAACGCCGCT
3B3	Kohara Phage	Genobase	PMID:3038334
uvrC279::Tn10	Linked marker	CAG12156 = CGSC7394^[4]	est. P1 cotransduction: 37% ^[5]
zed-3069::Tn10	Linked marker	CAG18451 = CGSC7395^[4]	est. P1 cotransduction: 47% ^[5]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G379	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002694	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946449	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4165	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006464	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FliK
Synonyms	flagellar hook-length control protein^[1], B1943^[2]^[1], FlaR^[2]^[1], FlaE^[2]^[1], FliK^[2]^[1] , ECK1941, flaE, JW1927, b1943
Product description	flagellar hook-length control protein FliK^[2]^[3]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003774	motor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001635	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009296	flagellum assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001635	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009424	bacterial-type flagellum hook	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001635	C	Seeded from EcoCyc (v14.0)	complete
	GO:0071973	bacterial-type flagellar cell motility	PMID:8631687	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0009424	bacterial-type flagellum hook	PMID:16949608	IMP: Inferred from Mutant Phenotype		C		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rsuA	PMID:16606699	Experiment(s):EBI-1141461
Protein	cydC	PMID:16606699	Experiment(s):EBI-1141461
Protein	pinE	PMID:16606699	Experiment(s):EBI-1141461
Protein	nuoE	PMID:16606699	Experiment(s):EBI-1141461
Protein	nadE	PMID:16606699	Experiment(s):EBI-1141461
Protein	purL	PMID:16606699	Experiment(s):EBI-1141461
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1141461
Protein	rhsC	PMID:16606699	Experiment(s):EBI-1141461
Protein	rpsA	PMID:16606699	Experiment(s):EBI-1141461
Protein	rplF	PMID:16606699	Experiment(s):EBI-1141461
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIRLAPLITA DVDTTTLPGG KASDAAQDFL ALLSEALAGE TTTDKAAPQL LVATDKPTTK GEPLISDIVS DAQQANLLIP VDETPPVIND EQSTSTPLTT AQTMALAAVA DKNTTKDEKA DDLNEDVTAS LSALFAMLPG FDNTPKVTDA PSTVLPTEKP TLFTKLTSEQ LTTAQPDDAP GTPAQPLTPL VAEAQSKAEV ISTPSPVTAA ASPLITPHQT QPLPTVAAPV LSAPLGSHEW QQSLSQHISL FTRQGQQSAE LRLHPQDLGE VQISLKVDDN QAQIQMVSPH QHVRAALEAA LPVLRTQLAE SGIQLGQSNI SGESFSGQQQ AASQQQQSQR TANHEPLAGE DDDTLPVPVS LQGRVTGNSG VDIFA
Length	375
Mol. Wt	39.311 kDa
pI	4.2 (calculated)
Extinction coefficient	5,500 (calc based on 0 Y, 1 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	245..336	PF02120 Flagellar hook-length control protein FliK		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fliK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129890	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946449	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006464	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P52614	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G379	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20268	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946449	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002694	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4165	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	6^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	5	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fliFGHIJK
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2016390..2016430 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1943 (EcoliWiki Page)	NCBI GEO profiles for fliK
microarray	GenExpDB:b1943 (EcoliWiki Page)	Summary of data for fliK from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fliK Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G379	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4165	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1943
EcoGene	EcoGene:EG20268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002694	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006464	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FLIK	From SHIGELLACYC
E. coli O157	FLIK	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02120 Flagellar hook-length control protein FliK
EcoCyc	EcoCyc:G379	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG20268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002694	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4165	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006464	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

fliK:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fliK Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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