eutC:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

eutC

Mnemonic

Ethanolamine utilization

Synonyms

ECK2435, b2440, JW2433[1], JW2433

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

55.06 minutes, 55.06 minutes 

MG1655: 2555319..2554432
<gbrowseImage> name=NC_000913:2554432..2555319 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2484816..2483929
<gbrowseImage> name=NC_012967:2483929..2484816 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2440237..2441124
<gbrowseImage> name=NC_012759:2440237..2441124 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2562743..2561856
<gbrowseImage> name=NC_007779:2561856..2562743 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2647084..2646197
<gbrowseImage> name=NC_010473:2646197..2647084 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2554432

Edman degradation

PMID:2197274[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔeutC (Keio:JW2433)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9939[4]

eutC::Tn5KAN-2 (FB20802)

Insertion at nt 221 in Minus orientation

PMID:15262929[5]

E. coli Genome Project:FB20802

contains pKD46

ΔeutC769::kan

PMID:16738554[3]

CGSC:100742


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2433

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGATCAAAAACAGATTGAAGA

Primer 2:CCTCGGGTCATGTTGATGCCGGA

E8E3

Kohara Phage

Genobase

PMID:3038334[7]

4E10

Kohara Phage

Genobase

PMID:3038334[7]

nupC510::Tn10

Linked marker

CAG18468 = CGSC7410[4]

est. P1 cotransduction: 14% [8]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: 19% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50007

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG50007

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002566

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946925

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4300

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008043

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Faust, LR et al. (1990) Cloning, sequencing, and expression of the genes encoding the adenosylcobalamin-dependent ethanolamine ammonia-lyase of Salmonella typhimurium. J. Biol. Chem. 265 12462-6 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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