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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	envC
Gene Synonym(s)	ECK3603, b3613, JW5646, yibP, slm11^[1]^[2], slm11
Product Desc.	EnvC murein hydrolase^[2]^[3] Periplasmic murein hydrolase septal ring factor; sensitivity to crystal violet; filamentous^[4]
Product Synonyms(s)	protease with a role in cell division^[1], B3613^[2]^[1], EnvC^[2]^[1], YibP^[2]^[1] , ECK3603, JW5646, slm11, yibP, b3613
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gpmI^[2]
Regulation/Activity
Quick Links
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Notes

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NlpD, YgeR, YebA and EnvC are paralogs. envC is a synthetic lethal with minCDE. EnvC is recruited to the septal ring and is needed for FtsZ ring formation at 42C. EnvC has in vitro protease activity against beta-casein. First 34 aa are predicted to be a type I signal peptide.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	envC
Mnemonic	Envelope
Synonyms	ECK3603, b3613, JW5646, yibP, slm11^[1]^[2], slm11
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	81.58 minutes	MG1655: 3784861..3786120 <gbrowseImage> name=NC_000913:3784861..3786120 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3727604..3728863 <gbrowseImage> name=NC_012967:3727604..3728863 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3673187..3674446 <gbrowseImage> name=NC_012759:3673187..3674446 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3853577..3852318 <gbrowseImage> name=NC_007779:3852318..3853577 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3882438..3883697 <gbrowseImage> name=NC_010473:3882438..3883697 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔenvC (Keio:JW5646)	deletion	deletion			PMID:16738554	Shigen CGSC11498^[5]
yibP::Tn5KAN-2 (FB21343)	Insertion at nt 1087 in Plus orientation				PMID:15262929	E. coli Genome Project:FB21343	does not contain pKD46
yibP::Tn5KAN-2 (FB21344)	Insertion at nt 1087 in Plus orientation				PMID:15262929	E. coli Genome Project:FB21344	contains pKD46
ΔyibP::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
ΔyibP::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
envC61						CGSC:11209
ΔenvC725::kan					PMID:16738554	CGSC:100720
ΔenvC	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔnlpD	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔyebA	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔygeR	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔnlpD ΔyebA	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔnlpD ΔygeR	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔyebA ΔygeR	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
ΔenvC ΔyebA ΔygeR ΔnlpD	deletion	deletion	Cell Shape	Defect in Cell Separation	PMID:19525345
envC	deletion		Sensitivity to	increases sensitivity to bicyclomycin	PMID:21357484		fig 2
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Notes

In envC mutants, constriction of the OM lags behind the IM and the FtsZ ring.

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5646	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACACGtGCCGTGAAACCGCG Primer 2:CCTCTTCCCAACCACGGCTGTGG
16B11	Kohara Phage	Genobase	PMID:3038334
5D2	Kohara Phage	Genobase	PMID:3038334
zhg-50::Tn10	Linked marker	CAG18450 = CGSC7450^[5]	est. P1 cotransduction: % ^[6] Synonyms:zhf-50::Tn10, zhf-5::Tn10
zic-4901::Tn10	Linked marker	CAG18492 = CGSC7457^[5]	est. P1 cotransduction: 16% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12297	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12297	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002178	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948129	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2205	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011820	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	EnvC
Synonyms	protease with a role in cell division^[1], B3613^[2]^[1], EnvC^[2]^[1], YibP^[2]^[1] , ECK3603, JW5646, slm11, yibP, b3613
Product description	EnvC murein hydrolase^[2]^[3] Periplasmic murein hydrolase septal ring factor; sensitivity to crystal violet; filamentous^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0042493	response to drug	PMID:8407802	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0016787	hydrolase activity	PMID:15165230	IDA: Inferred from Direct Assay		F	murein hydrolase activity	complete
	GO:0042597	periplasmic space	PMID:15165230	IMP: Inferred from Mutant Phenotype		C		complete
	GO:0000920	cytokinetic cell separation	PMID:19525345	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0000920	cytokinetic cell separation	PMID:19525345	IGI: Inferred from Genetic Interaction	EcoliWiki:NlpD	P		complete
	GO:0004222	metalloendopeptidase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002886	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006508	proteolysis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002886	P	Seeded from EcoCyc (v14.0)	complete
	GO:0032155	cell division site part	PMID:19525345	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0032155	cell division site part	PMID:19525345	IDA: Inferred from Direct Assay		C	shows midcell localization in both short unconstricted cells and longer cells with a constriction	complete
	GO:0000920	cytokinetic cell separation	PMID:19525345	IGI: Inferred from Genetic Interaction	EcoliWiki:nlpD\|EcoliWiki:yebA\|EcoliWiki:ygeR	P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Inner Membrane	PMID:8472900	EchoLocation:envC
periplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRGKAINTMT RAVKPRRFAI RPIIYASVLS AGVLLCAFSA HADERDQLKS IQADIAAKER AVRQKQQQRA SLLAQLKKQE EAISEATRKL RETQNTLNQL NKQIDEMNAS IAKLEQQKAA QERSLAAQLD AAFRQGEHTG IQLILSGEES QRGQRLQAYF GYLNQARQET IAQLKQTREE VAMQRAELEE KQSEQQTLLY EQRAQQAKLT QALNERKKTL AGLESSIQQG QQQLSELRAN ESRLRNSIAR AEAAAKARAE REAREAQAVR DRQKEATRKG TTYKPTESEK SLMSRTGGLG APRGQAFWPV RGPTLHRYGE QLQGELRWKG MVIGASEGTE VKAIADGRVI LADWLQGYGL VVVVEHGKGD MSLYGYNQSA LVSVGSQVRA GQPIALVGSS GGQGRPSLYF EIRRQGQAVN PQPWLGR
Length	427
Mol. Wt	47.466 kDa
pI	10.4 (calculated)
Extinction coefficient	36,900 - 37,025 (calc based on 10 Y, 4 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	319..413	PF01551 Peptidase family M23		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=envC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:162135913	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948129	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011820	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37690	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12297	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12297	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948129	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002178	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2205	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	258	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	106	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	165	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gpmI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3784841..3784881 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3613 (EcoliWiki Page)	NCBI GEO profiles for envC
microarray	GenExpDB:b3613 (EcoliWiki Page)	Summary of data for envC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to envC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12297	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2205	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3613
EcoGene	EcoGene:EG12297	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002178	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011820	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000034224 (score: 1.000; bootstrap: 95%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G16000 (score: 1.000; bootstrap: 61%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000019358 (score: 1.000; bootstrap: 100%) ENSCINP00000019902 (score: 1.000; bootstrap: 100%) ENSCINP00000019916 (score: 0.976) ENSCINP00000002997 (score: 0.095) ENSCINP00000019026 (score: 0.072) ENSCINP00000001565 (score: 0.068) ENSCINP00000016764 (score: 0.066) ENSCINP00000000142 (score: 0.054) ENSCINP00000000143 (score: 0.051)	From Inparanoid:20070104
Homo sapiens	ENSP00000261535 (score: 1.000; bootstrap: 63%) ENSP00000317985 (score: 0.646) ENSP00000354818 (score: 0.158) ENSP00000295191 (score: 0.155) ENSP00000261833 (score: 0.155) ENSP00000346168 (score: 0.106) ENSP00000282893 (score: 0.085) ENSP00000229812 (score: 0.082) ENSP00000319504 (score: 0.061) ENSP00000309591 (score: 0.059) ENSP00000297734 (score: 0.058) ENSP00000262848 (score: 0.057)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000006193 (score: 1.000; bootstrap: 62%) ENSMMUP00000021934 (score: 0.543) ENSMMUP00000011339 (score: 0.170) ENSMMUP00000010991 (score: 0.160) ENSMMUP00000026115 (score: 0.139) ENSMMUP00000021623 (score: 0.119) ENSMMUP00000030594 (score: 0.080) ENSMMUP00000004604 (score: 0.066) ENSMMUP00000026815 (score: 0.059) ENSMMUP00000004834 (score: 0.055) ENSMMUP00000026292 (score: 0.054) ENSMMUP00000002844 (score: 0.053) ENSMMUP00000001399 (score: 0.051) ENSMMUP00000010660 (score: 0.051) ENSMMUP00000011453 (score: 0.051)	From Inparanoid:20070104
Oryza gramene	Q6AT30 (score: 1.000; bootstrap: 49%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000016847 (score: 1.000; bootstrap: 68%) ENSPTRP00000041202 (score: 0.693) ENSPTRP00000003469 (score: 0.235) ENSPTRP00000041655 (score: 0.191) ENSPTRP00000008170 (score: 0.152) ENSPTRP00000030929 (score: 0.146) ENSPTRP00000042330 (score: 0.109) ENSPTRP00000035898 (score: 0.104) ENSPTRP00000018062 (score: 0.090) ENSPTRP00000018854 (score: 0.088) ENSPTRP00000031907 (score: 0.087) ENSPTRP00000016236 (score: 0.081) ENSPTRP00000034767 (score: 0.081) ENSPTRP00000036653 (score: 0.077) ENSPTRP00000023198 (score: 0.075) ENSPTRP00000025952 (score: 0.070) ENSPTRP00000006810 (score: 0.070) ENSPTRP00000011570 (score: 0.069) ENSPTRP00000019651 (score: 0.068) ENSPTRP00000001604 (score: 0.055) ENSPTRP00000000076 (score: 0.051)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000139983 (score: 1.000; bootstrap: 71%) NEWSINFRUP00000159409 (score: 0.086) NEWSINFRUP00000154559 (score: 0.085) NEWSINFRUP00000143504 (score: 0.051)	From Inparanoid:20070104
Shigella flexneri	YIBP	From SHIGELLACYC
E. coli O157	YIBP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01551 Peptidase family M23
Superfamily (EcoliWiki Page)	SUPERFAMILY:51261
Panther (EcoliWiki Page)	PTHR21666:SF26
EcoCyc	EcoCyc:EG12297	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12297	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002178	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2205	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011820	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

envC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to envC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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