entB:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

entB

Gene Synonym(s)

ECK0588, b0595, JW0587, entG[1], entG

Product Desc.

EntB[2][3]

Asochorismatase, aryl carrier protein (ArCP); also called 2,3-dihydro-2,3-dihydroxybenzoate synthase[4]

Product Synonyms(s)

isochorismatase[1], B0595[2][1], EntG[2][1], EntB[2][1] , ECK0588, entG, JW0587, b0595

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): entCEBA-ybdB[2], OP00227

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

entB

Mnemonic

Enterochelin

Synonyms

ECK0588, b0595, JW0587, entG[1], entG

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.51 minutes 

MG1655: 626917..627774
<gbrowseImage> name=NC_000913:626917..627774 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 610402..611259
<gbrowseImage> name=NC_012967:610402..611259 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 529677..530534
<gbrowseImage> name=NC_012759:529677..530534 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 626917..627774
<gbrowseImage> name=NC_007779:626917..627774 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 566249..567106
<gbrowseImage> name=NC_010473:566249..567106 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 679509..680366
<gbrowseImage> name=NC_010473:679509..680366 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

entB(del) (Keio:JW0587)

deletion

deletion

PMID:16738554

Shigen
CGSC8704[5]

entB733(del)::kan

PMID:16738554

CGSC:100712

entB(del)C

deletion

DELETION of C-terminal 25 amino acids of entB

Auxotrophies

deletion disrupts production of 2,3-Dihydrobenzoate which is an important intermediate of Enterobactin synthesis.

PMID:9214294

Figure 3.

entB::Tn5 entG at the beginning of entB

insertion of Tn5 198 base pairs from entB termination codon

Auxotrophies

The coding sequence for EntG activity is found with in the entB 3' terminus. When EntB is disrupted then s is EntG. (table 2)

PMID 2172214

Strain: AB1515

entB::Tn5 entG at the end of entB

an insertion of a Tn5 at the end of entB sequence

Auxotrophies

No effect on entG activity

PMID 2172214

Strain: AB1515

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0587

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTATTCCAAAATTACAGGC

Primer 2:CCTTTCACCTCGCGGGAGAGTAG

25A7

Kohara Phage

Genobase

PMID:3038334

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[5]

est. P1 cotransduction: 1% [6]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 74% [6]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10260

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10260

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000254

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946178

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0256

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002052

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EntB

Synonyms

isochorismatase[1], B0595[2][1], EntG[2][1], EntB[2][1] , ECK0588, entG, JW0587, b0595

Product description

EntB[2][3]

Asochorismatase, aryl carrier protein (ArCP); also called 2,3-dihydro-2,3-dihydroxybenzoate synthase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Deprecated

GO:0000036

acyl carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009081


F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000868

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006811

ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc (v14.0)

complete

GO:0006826

iron ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0410

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000868

P

Seeded from EcoCyc (v14.0)

complete

GO:0008908

isochorismatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003881

F

Seeded from EcoCyc (v14.0)

complete

GO:0008908

isochorismatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.3.2.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0009058

biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003881

P

Seeded from EcoCyc (v14.0)

complete

GO:0009239

enterobactin biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0259

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0048037

cofactor binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006163

F

Seeded from EcoCyc (v14.0)

complete

GO:0016765

transferase activity, transferring alkyl or aryl (other than methyl) groups

PMID:9214294

IMP: Inferred from Mutant Phenotype

F

See figure 3.

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

glpD

PMID:16606699

Experiment(s):EBI-1137295

Protein

uspG

PMID:16606699

Experiment(s):EBI-1137295

Protein

rplF

PMID:16606699

Experiment(s):EBI-1137295

Protein

yhjG

PMID:16606699

Experiment(s):EBI-1137295

Protein

dld

PMID:16606699

Experiment(s):EBI-1137295

Protein

groL

PMID:16606699

Experiment(s):EBI-1137295

Protein

infB

PMID:16606699

Experiment(s):EBI-1137295

Protein

nadE

PMID:16606699

Experiment(s):EBI-1137295

Protein

adk

PMID:16606699

Experiment(s):EBI-1137295

Protein

lacZ

PMID:16606699

Experiment(s):EBI-1137295

Protein

pepD

PMID:16606699

Experiment(s):EBI-1137295

Protein

rplN

PMID:16606699

Experiment(s):EBI-1137295

Protein

gltB

PMID:16606699

Experiment(s):EBI-1137295

Protein

mukB

PMID:16606699

Experiment(s):EBI-1137295

Protein

lon

PMID:16606699

Experiment(s):EBI-1137295

Protein

rhsC

PMID:16606699

Experiment(s):EBI-1137295
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI
AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV
LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD
FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID
YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK
Length

285

Mol. Wt

32.554 kDa

pI

5.0 (calculated)

Extinction coefficient

51,910 - 52,285 (calc based on 9 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

31..205

PF00857 Isochorismatase family

PMID:19920124

Domain

216..280

PF00550 Phosphopantetheine attachment site

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=entB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128578

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946178

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002052

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ADI4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10260

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10260

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946178

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000254

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0256

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

8.21E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

59

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

62

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

6a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

entCEBA-ybdB

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:626897..626937 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0595 (EcoliWiki Page)

NCBI GEO profiles for entB

microarray

GenExpDB:b0595 (EcoliWiki Page)

Summary of data for entB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to entB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10260

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0256

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0595

EcoGene

EcoGene:EG10260

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000254

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002052

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT5G23230 (score: 1.000; bootstrap: 100%)
  • AT5G23220 (score: 0.837)

From Inparanoid:20070104

Oryza gramene

  • Q7XKJ8 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000006738 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ENTB

From SHIGELLACYC

E. coli O157

ENTB

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00857 Isochorismatase family

Superfamily (EcoliWiki Page)

SUPERFAMILY:47336

Superfamily (EcoliWiki Page)

SUPERFAMILY:52499

Panther (EcoliWiki Page)

PTHR11080:SF4

Pfam (EcoliWiki Page)

PF00550 Phosphopantetheine attachment site

EcoCyc

EcoCyc:EG10260

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10260

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000254

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0256

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002052

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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