entB:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
entB |
|---|---|
| Mnemonic |
Enterochelin |
| Synonyms |
ECK0588, b0595, JW0587, entG[1], entG |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
13.51 minutes |
MG1655: 626917..627774 |
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NC_012967: 610402..611259 |
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NC_012759: 529677..530534 |
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W3110 |
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W3110: 626917..627774 |
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DH10B: 566249..567106 |
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DH10B: 679509..680366 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
entB(del) (Keio:JW0587) |
deletion |
deletion |
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entB733(del)::kan |
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entB(del)C |
deletion |
DELETION of C-terminal 25 amino acids of entB |
Auxotrophies |
deletion disrupts production of 2,3-Dihydrobenzoate which is an important intermediate of Enterobactin synthesis. |
Figure 3. | ||
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entB::Tn5 entG at the beginning of entB |
insertion of Tn5 198 base pairs from entB termination codon |
Auxotrophies |
The coding sequence for EntG activity is found with in the entB 3' terminus. When EntB is disrupted then s is EntG. (table 2) |
PMID 2172214 |
Strain: AB1515 | ||
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entB::Tn5 entG at the end of entB |
an insertion of a Tn5 at the end of entB sequence |
Auxotrophies |
No effect on entG activity |
PMID 2172214 |
Strain: AB1515 | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0587 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCTATTCCAAAATTACAGGC Primer 2:CCTTTCACCTCGCGGGAGAGTAG | |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 1% [7] | ||
|
Linked marker |
est. P1 cotransduction: 74% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Gehring, AM et al. (1997) Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate. Biochemistry 36 8495-503 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
