entB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

EntB

Synonyms

isochorismatase[1], B0595[2][1], EntG[2][1], EntB[2][1] , ECK0588, entG, JW0587, b0595

Product description

EntB[2][3]

Asochorismatase, aryl carrier protein (ArCP); also called 2,3-dihydro-2,3-dihydroxybenzoate synthase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

Deprecated

GO:0000036

acyl carrier activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009081


F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000868

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0408

F

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0006811

ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc (v14.0)

complete

GO:0006826

iron ion transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0410

P

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000868

P

Seeded from EcoCyc (v14.0)

complete

GO:0008908

isochorismatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003881

F

Seeded from EcoCyc (v14.0)

complete

GO:0008908

isochorismatase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:3.3.2.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0009058

biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003881

P

Seeded from EcoCyc (v14.0)

complete

GO:0009239

enterobactin biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0259

P

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0048037

cofactor binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006163

F

Seeded from EcoCyc (v14.0)

complete

GO:0016765

transferase activity, transferring alkyl or aryl (other than methyl) groups

PMID:9214294[5]

IMP: Inferred from Mutant Phenotype

F

See figure 3.

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

glpD

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

uspG

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

rplF

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

yhjG

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

dld

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

groL

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

infB

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

adk

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

lacZ

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

pepD

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

rplN

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

gltB

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

mukB

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

lon

PMID:16606699[6]

Experiment(s):EBI-1137295

Protein

rhsC

PMID:16606699[6]

Experiment(s):EBI-1137295
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI
AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV
LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD
FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID
YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK
Length

285

Mol. Wt

32.554 kDa

pI

5.0 (calculated)

Extinction coefficient

51,910 - 52,285 (calc based on 9 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

31..205

PF00857 Isochorismatase family

PMID:19920124[7]

Domain

216..280

PF00550 Phosphopantetheine attachment site

PMID:19920124[7]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=entB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128578

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946178

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002052

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ADI4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10260

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10260

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946178

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000254

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0256

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Gehring, AM et al. (1997) Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate. Biochemistry 36 8495-503 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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