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Gene |

Product(s) |

Expression|

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Standard Name	dsbG
Gene Synonym(s)	ECK0598, b0604, JW0597, ybdP^[1], ybdP
Product Desc.	periplasmic disulfide isomerase/thiol-disulphide oxidase^[1] Thiol-disulphide oxidase; molecular chaperone; multicopy resistance to DTT; mutants accumulate reduced proteins^[2]
Product Synonyms(s)	B0604^[3]^[1], YbdP^[3]^[1] , ECK0598, JW0597, ybdP, b0604
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dsbG^[3]
Regulation/Activity
Quick Links
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Notes

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A dsbG defect iscorrected by DsbAB overexpression.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dsbG
Mnemonic	Disulfide bond
Synonyms	ECK0598, b0604, JW0597, ybdP^[1], ybdP
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	13.73 minutes	MG1655: 637796..637050 <gbrowseImage> name=NC_000913:637050..637796 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 620624..619863 <gbrowseImage> name=NC_012967:619863..620624 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 539810..540556 <gbrowseImage> name=NC_012759:539810..540556 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 637796..637050 <gbrowseImage> name=NC_007779:637050..637796 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 577128..576382 <gbrowseImage> name=NC_010473:576382..577128 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
DH10B		DH10B: 690388..689642 <gbrowseImage> name=NC_010473:689642..690388 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	637053	Edman degradation	PMID:9383195
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔdsbG (Keio:JW0597)	deletion	deletion		PMID:16738554	Shigen CGSC8321^[4]
dsbGC129A		C129A	Partial loss of redox activity			seeded from UniProt:P77202
dsbGC129S		C129S	No loss of chaperone activity; when associated with S-126			seeded from UniProt:P77202
dsbGC126A		C126A	Complete loss of redox activity			seeded from UniProt:P77202
dsbGC126S		C126S	No loss of chaperone activity; when associated with S-129			seeded from UniProt:P77202
dsbG601::Tn10	Insertion at 637,765 bp in MG1655 (NC_000913)			adapted from Nichols et al.^[5]	CAG12149 = CGSC7346^[4]	Synonyms: zbd-601::Tn10, zbe-601::Tn10
dsbG601::Tn10				PMID:2540407 PMID:9829956	CGSC:5072
ΔdsbG743::kan				PMID:16738554	CGSC:100654
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0597	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTAAAAAAGATACTTTTACT Primer 2:CCTTTATTCCCCATAATGATATT
4A5	Kohara Phage	Genobase	PMID:3038334
3G5	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[4]	est. P1 cotransduction: % ^[5] Synonyms:purE79::Tn10
crcA280::Tn10	Linked marker	CAG12077 = CGSC7347^[4]	est. P1 cotransduction: 54% ^[5] Synonyms:zbe-280::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6333	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13535	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002940	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945224	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3306	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002084	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DsbG
Synonyms	B0604^[3]^[1], YbdP^[3]^[1] , ECK0598, JW0597, ybdP, b0604
Product description	periplasmic disulfide isomerase/thiol-disulphide oxidase^[1] Thiol-disulphide oxidase; molecular chaperone; multicopy resistance to DTT; mutants accumulate reduced proteins^[2]
EC number (for enzymes)	5.3.4.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0003756	protein disulfide isomerase activity	PMID:10075670	IGI: Inferred from Genetic Interaction		F	Seeded from EcoCyc (v14.0)	Missing: with/from
	GO:0003756	protein disulfide isomerase activity	PMID:9383195	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0015035	protein disulfide oxidoreductase activity	PMID:19965429	IMP: Inferred from Mutant Phenotype		F	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	adiY	PMID:16606699	Experiment(s):EBI-1137364
Protein	mnmG	PMID:16606699	Experiment(s):EBI-1137364
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1137364
Protein	yfeT	PMID:16606699	Experiment(s):EBI-1137364
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1137364
Protein	rplC	PMID:16606699	Experiment(s):EBI-1137364
Protein	cysJ	PMID:19402753	LCMS(ID Probability):99.6
Protein	cysM	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[6]
Periplasm	PMID:9383195	EchoLocation:dsbG
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTVIGYAFYS TFALTEKDKL MLKKILLLAL LPAIAFAEEL PAPVKAIEKQ GITIIKTFDA PGGMKGYLGK YQDMGVTIYL TPDGKHAISG YMYNEKGENL SNTLIEKEIY APAGREMWQR MEQSHWLLDG KKDAPVIVYV FADPFCPYCK QFWQQARPWV DSGKVQLRTL LVGVIKPESP ATAAAILASK DPAKTWQQYE ASGGKLKLNV PANVSTEQMK VLSDNEKLMD DLGANVTPAI YYMSKENTLQ QAVGLPDQKT LNIIMGNK
Length	268
Mol. Wt	29.776 kDa
pI	8.4 (calculated)
Extinction coefficient	46,870 - 47,120 (calc based on 13 Y, 5 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-17	UniProt Manual:Signal Peptides	UniProt:P77202
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dsbG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111151	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945224	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002084	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77202	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6333	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13535	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945224	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002940	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3306	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.12E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	15.511+/-0.15	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.032841823	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	543	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	229	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	278	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dsbG
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:637776..637816 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0604 (EcoliWiki Page)	NCBI GEO profiles for dsbG
microarray	GenExpDB:b0604 (EcoliWiki Page)	Summary of data for dsbG from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (637759..638206) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:H8^[7]
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Notes

Accessions Related to dsbG Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6333	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3306	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0604
EcoGene	EcoGene:EG13535	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002940	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002084	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	DSBG	From SHIGELLACYC
E. coli O157	DSBG	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 ^4.3 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 ^5.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

dsbG:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dsbG Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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