dnaK:On One Page

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

<protect>

Standard Name

dnaK

Gene Synonym(s)

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

Product Desc.

chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins[2][3];

Component of DnaJ/DnaK/GrpE[2][3]

Hsp70 molecular chaperone, heat-inducible[4]

Product Synonyms(s)

chaperone Hsp70, co-chaperone with DnaJ[1], B0014[2][1], GrpC[2][1], GrpF[2][1], Seg[2][1], GroP[2][1], DnaL[2][1], DnaK[2][1] , ECK0014, groPAB, groPC, groPF, grpC, grpF, JW0013, seg, b0014

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dnaKJ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]


ATP-regulated binding and release of polypeptide substrates. Binds Zn(II). DnaJ is co-chaperone. Role in the initiation of DNA replication. DnaK has secondary amide peptide bond cis-trans isomerase (APIase) activity. A dnaK mutant is inviable above 42C, or below 8C (as enabled by transgenic Oleispira antarctica Cpn10/60). Delta tig delta dnaK mutants are synthetic lethal at 30 degrees C, however, no lethal effects are observed at 23 degrees C; overproduction of GroEL/GroES at 30 degrees C supresses synthetic lethality. dnaK, hscA, hscC and yegD are paralogs. HT_Cmplx22_Cyt: DnaK+GrpE+GyrA+RpsA. HT_Cmplx30_Mem: ClpB+DnaK+InfB+RpsA. After four hours of Zn(II) stress, protein levels increased (Easton, 2006).[4]



Gene

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaK

Mnemonic

DNA

Synonyms

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.26 minutes 

MG1655: 12163..14079
<gbrowseImage> name=NC_000913:12163..14079 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 12161..14077
<gbrowseImage> name=NC_012967:12161..14077 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 12163..14079
<gbrowseImage> name=NC_012759:12163..14079 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 12163..14079
<gbrowseImage> name=NC_007779:12163..14079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 12163..14079
<gbrowseImage> name=NC_010473:12163..14079 source=DH10B preset=GeneLocation </gbrowseImage>

edit table

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

12166

Edman degradation

PMID:1396676
PMID:7783627
PMID:9298646


edit table

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dnaK(del) (Keio:JW0013)

deletion

deletion

PMID:16738554

Shigen

CGSC8342[5]

dnaKG32D

G32D

In SEG-1 and dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKV436I

V436I

In mutant SEG-2; confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG455D

G455D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG468D

G468D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaK756(ts)

temperature sensitive

CGSC:6707

dnaK(del)734::kan

deletion

deletion

PMID:16738554

CGSC:100628

dnaK

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484

fig 2

DnaKG384A

Glycine to Alanine at position 384.

There is a 13 fold decrease rate for substrate release in ATP bound state and increase in substrate affinity. with an overall 2.5 fold higher chaperon activity, Table 1.

PMID:21656889

G384A/Cd35

Double mutant

The double mutant combines accelerated peptide binding in its ADP-bound state (like Cd35) and slower peptide release in its ATP-bound state (likeG384A), but without additivity in DnaK-assisted firefly luciferase refolding, table 1.

PMID:21656889

Cd35

35-residue C-terminal truncation

Cd35 showed a 7-fold increase in initial refolding rate for denatured luciferase and resulted in a 5-fold increase in maximal luminescence as compared to wild type DnaK, Table 3.

PMID:20381501

dnaK7

revertant

Mutant is Leu-. When Leu-auxotrophs of Escherichia coli after UV irradiation, were grown at temperatures between 30 and 47C the frequent of UV-induced mutation from Leu- to Leu+ increased as UV dose and temperature increased. Figure 2.

PMID:22270474


edit table

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0013

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGGTAAAATAATTGGTATCGA

Primer 2:CCTTTTTTGTCTTTGACTTCTTC

9E4

Kohara Phage

Genobase

PMID:3038334

6H3

Kohara Phage

Genobase

PMID:3038334

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 66% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 48% [6]
Synonyms:car-96::Tn10

edit table

<protect></protect>

Notes

Sugimoto et al [7] constructed a strain carrying a dnaK deletion and the lambda DE3 prophage, which expresses T7 RNA polymerase under lac control.

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944750

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



Product(s)

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DnaK

Synonyms

chaperone Hsp70, co-chaperone with DnaJ[1], B0014[2][1], GrpC[2][1], GrpF[2][1], Seg[2][1], GroP[2][1], DnaL[2][1], DnaK[2][1] , ECK0014, groPAB, groPC, groPF, grpC, grpF, JW0013, seg, b0014

Product description

chaperone Hsp70; DNA biosynthesis; autoregulated heat shock proteins[2][3];

Component of DnaJ/DnaK/GrpE[2][3]

Hsp70 molecular chaperone, heat-inducible[4]

EC number (for enzymes)


edit table

<protect></protect>

Notes

Function

[back to top]


<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:10521435

IPI: Inferred from Physical Interaction

UniProtKB:P08622

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00332

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001023

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012725

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012725

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012725

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:11985624

IDA: Inferred from Direct Assay

F

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of DnaJ/DnaK/GrpE

could be indirect

Protein

htpG

PMID:16606699

Experiment(s):EBI-1135316

Protein

aphA

PMID:15690043

Experiment(s):EBI-890853

Protein

gadA

PMID:15690043

Experiment(s):EBI-883577, EBI-888455, EBI-893820

Protein

grpE

PMID:15690043

Experiment(s):EBI-883577, EBI-881166, EBI-888219, EBI-890853

Protein

lon

PMID:15690043

Experiment(s):EBI-883577

Protein

rplF

PMID:15690043

Experiment(s):EBI-883577

Protein

rplJ

PMID:15690043

Experiment(s):EBI-883577

Protein

rpsB

PMID:15690043

Experiment(s):EBI-883577, EBI-890853

Protein

tufA

PMID:15690043

Experiment(s):EBI-883577, EBI-894882, EBI-890853

Protein

yibA

PMID:15690043

Experiment(s):EBI-883577

Protein

ydcR

PMID:15690043

Experiment(s):EBI-890853

Protein

ydfJ

PMID:15690043

Experiment(s):EBI-890853

Protein

entB

PMID:15690043

Experiment(s):EBI-890853

Protein

fabB

PMID:15690043

Experiment(s):EBI-890853

Protein

hisC

PMID:15690043

Experiment(s):EBI-890853

Protein

hscA

PMID:15690043

Experiment(s):EBI-890853

Protein

narQ

PMID:15690043

Experiment(s):EBI-890853

Protein

pepB

PMID:15690043

Experiment(s):EBI-890853

Protein

proS

PMID:15690043

Experiment(s):EBI-890853

Protein

rplI

PMID:15690043

Experiment(s):EBI-890853

Protein

rplS

PMID:15690043

Experiment(s):EBI-890853

Protein

rpmG

PMID:15690043

Experiment(s):EBI-890853

Protein

sapA

PMID:15690043

Experiment(s):EBI-890853

Protein

tdcG

PMID:15690043

Experiment(s):EBI-890853

Protein

dcuD

PMID:15690043

Experiment(s):EBI-890853

Protein

yihM

PMID:15690043

Experiment(s):EBI-890853
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytoplasm

PMID:9298646, PMID:1396676, PMID:7783627

EchoLocation:dnaK


edit table

<protect></protect>

Notes

Structure and Physical Properties

[back to top]


<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVT
NPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVL
KKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIIN EPTAAALAYG
LDKGTGNRTI AVYDLGGGTF DISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYL
VEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKV
TRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKE
PRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPT
KHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMP QIEVTFDIDA
DGILHVSAKD KNSGKEQKIT IKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGD
HLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLME
IAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK
Length

638

Mol. Wt

69.115 kDa

pI

4.7 (calculated)

Extinction coefficient

15,930 - 16,055 (calc based on 7 Y, 1 W, and 1 C residues)

edit table


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6Y8

Modification Site

453

phosphorylation site at S453

probability greater than 75%

PMID:17938405

Domain

4..604

PF00012 Hsp70 protein

PMID:19920124

edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dnaK taxon=562,83333 </beststructure>

Models

View models at:

edit table
</protect>

Structure figures

<protect>

</protect>

Notes

Like other Hsp70 chaperones, dnaK consists of two domains: and N-terminal ATPase domain, a substrate binding domain, and a short DnaJ binding domain[8]. Structures

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
edit table

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128008

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6Y8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944750

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944750

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]




Expression

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

[back to top]


See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

3.12E+04

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12

2435.911+/-10.089

molecules/cell

  • Medium:

single molecule fluorescence

PMID:20671182

mRNA

E. coli K-12

0.64195+/-0.02703

molecules/cell

  • Medium:

FISH

PMID:20671182

mRNA

E. coli K-12

0.494780793

molecules/cell

  • Medium:

RNA_Seq

PMID:20671182

Protein

E. coli K-12 EMG2

1300

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M43

PMID: 9298646

Protein

E. coli K-12 MG1655

44581

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

10567

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

45951

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

edit table

Notes

Transcription and Transcriptional Regulation

[back to top]


<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dnaKJ

edit table
Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]


<protect><gbrowseImage> name=NC_000913:12143..12183 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
edit table


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0014 (EcoliWiki Page)

NCBI GEO profiles for dnaK

microarray

GenExpDB:b0014 (EcoliWiki Page)

Summary of data for dnaK from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (11706..12211) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ20; Well:E8[9]

edit table

<protect></protect>

Notes

Accessions Related to dnaK Expression

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0014

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



Evolution

[back to top]


{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]


See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000022995 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000007663 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT4G37910 (score: 1.000; bootstrap: 97%)
  • AT5G09590 (score: 0.570)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000015172 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00030552 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00002010 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000001849 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000013308 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030828-12 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-040425-391 (score: 1.000; bootstrap: 99%)
  • ZDB-CDNA-041024-1 (score: 0.988)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0215366 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0001220 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21150-PA (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000003717 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000297185 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000007731 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000029223 (score: 0.477)
  • ENSMMUP00000006030 (score: 0.171)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000015137 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Mus musculus

  • MGI:96245 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Oryza gramene

  • Q6Z7L1 (score: 1.000; bootstrap: 99%)
  • Q8GTB0 (score: 0.788)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000029565 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000026696 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YJR045C (score: 1.000; bootstrap: 99%)
  • YEL030W (score: 0.594)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC6641 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000171346 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00034701001 (score: 1.000; bootstrap: 80%)
  • GSTENP00034205001 (score: 0.312)
  • GSTENP00016340001 (score: 0.310)
  • GSTENP00007191001 (score: 0.239)
  • GSTENP00007193001 (score: 0.217)
  • GSTENP00028179001 (score: 0.124)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000018420 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

Shigella flexneri

DNAK

From SHIGELLACYC

E. coli O157

DNAK

From ECOO157CYC

edit table


Do-It-Yourself Web Tools

<protect></protect>

Notes

Families

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00012 Hsp70 protein

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Superfamily (EcoliWiki Page)

SUPERFAMILY:100920

EcoCyc

EcoCyc:EG10241

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10241

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000235

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0237

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000052

Escherichia coli str. K-12 substr. MG1655

edit table

<protect></protect>

Notes

Links

[back to top]



References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Sugimoto, S et al. (2008) Construction of Escherichia coli dnaK-deletion mutant infected by lambdaDE3 for overexpression and purification of recombinant GrpE proteins. Protein Expr. Purif. 60 31-6 PubMed
  8. ↑ Genevaux, P et al. (2007) The Hsp70 chaperone machines of Escherichia coli: a paradigm for the repartition of chaperone functions. Mol. Microbiol. 66 840-57 PubMed
  9. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

[back to top]


Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=dnaK:On_One_Page&oldid=218409"