lambda DE3
lambda DE3 is a phage construct that expresses T7 RNA polymerase under the control of a lacUV5 promoter.
Studier and Moffat[1] constructed lambda DE3 as one of a series of phage constructs where T7 gene 1 was cloned into the lambda D69[2] a unique BamH1. D69 is:
Bam10 srI(1-2)delta att+ imm21 nin5 shn6
In DE3, transcription of T7 gene 1 is rightward toward the int promoter. The BamH1 site where PlacUV5-T7 1 is inserted is in the int gene, so DE3 is an int mutant. Lysogens of DE3 are made using a helper phage of heterologous immunity. The int mutation should also prevent normal excision from lysogens.
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