dnaA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

dnaA

Gene Synonym(s)

ECK3694, b3702, JW3679[1], JW3679

Product Desc.

DnaA transcriptional dual regulator[2][3]

DNA synthesis initiator and global transcription regulator; binds DNA at DnaA boxes, binds cardiolipin and other acidic phospholipids, binds ATP[4]

Product Synonyms(s)

chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator[1], B3702[2][1], DnaA[2][1], DNA biosynthesis; DnaA-ATP initiates chromosome replication; can be transcription regulator[2][1] , ECK3694, JW3679, b3702

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dnaAN-recF[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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DnaA-ATP is the active form; DnaA-ADP is inactive; switching betwee the two forms modulates initiation activity. Acidic phospholipids release bound ADP, reactivating DnaA by allowing ATP to rebind. Null mutations in seqA, yccV or rpoN suppress dna46(Ts). Increases in DnaA levels reduce Fis oriC primary binding thus allowing DnaA to bind, this may play a role in the dynamics of prereplication complexes. Collapsed replication forks (primarily within 10 map units of oriC) are a result of the level of initiation induced by elevated dnaA expression. Hda-ADP is the monomeric active form for promoting clamp-mediated DNA-ATP hydrolysis (Su'etsugu, 2008).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaA

Mnemonic

DNA

Synonyms

ECK3694, b3702, JW3679[1], JW3679

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.63 minutes 

MG1655: 3881752..3880349
<gbrowseImage> name=NC_000913:3880349..3881752 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3844090..3842687
<gbrowseImage> name=NC_012967:3842687..3844090 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3768682..3770085
<gbrowseImage> name=NC_012759:3768682..3770085 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3756686..3758089
<gbrowseImage> name=NC_007779:3756686..3758089 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

P18S

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

dnaA177(ts)

temperature sensitive

CGSC:5231

dnaA46

A184V and H252Y

Growth Phenotype

Temperature-sensitive for initiation of DNA replication.

PMID:1495477

dnaA508(ts)

temperature sensitive

PMID:6094968

CGSC:5272

dnaA5(ts)

temperature sensitive

PMID:321430 PMID:6094968

CGSC:7325

dnaA46(ts)

783 C->T (where +1 is the txn'l start site)

Ala->Val

Growth Phenotype

temperature sensitive

PMID:6094968

CGSC:73497

Sequence of the mutant allele is from Ohmori et al.[5]

dnaAcos

dnaA1(Cs) 699 A->T dnaA46(Ts) 783 C->T dnaA2(Cs) 986 C->T dnaA3(Cs) 1043 T->C (Where +1 is the txn'l start site)

Gln->Leu Ala->Val His->Tyr Tyr->His

Growth Phenotype

over initiation of DNA replication at 30°C

PMID:9379896

dnaAcos was isolated as a temperature-resistant revertant of dnaA46(Ts). [6]

dnaA W6A

W6A

PMID:15878847

W6 is needed for oligomerization

dnaA311(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354

suppressed in supF, supE, supD, and supP strains

dnaA366(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354

  • ts in supF
  • suppressed in supE and supD strains

dnaA91(Am)

Growth Phenotype

inhibition of initiation of DNA replication

PMID:7007354

suppressed in supF, supD, and supP strains

dnaA204(Ts)

Resistant to

blocks resistance to camphor in all mbr mutants

PMID:2176633 PMID:6094968

dnaA167

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA211

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA606

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA601

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA604

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA603

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA205

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA602

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA203

Growth Phenotype

temperature sensitive

PMID:6094968

dnaA46

Resistant to

Increased resistance to rifampin.

PMID:400782

Parent Strain: EC3 X EC6

Experimental Strain: EC8

Mutant phenotype found at Table 2.

rpoB706 dnaA1

Growth Phenotype

DnaA1 Mutant is unable to grow at 42.5C, mutation in rpoB suppresses thermosensitivity allowing the strain to grow at 42.5C.

PMID:400782

Experimental Strain: EC8


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3679

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCACTTTCGCTTTGGCAGCA

Primer 2:CCaGATGACAATGTTCTGATTAA

3F11

Kohara Phage

Genobase

PMID:3038334

3C10

Kohara Phage

Genobase

PMID:3038334

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[7]

est. P1 cotransduction: 81% [8]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[7]

est. P1 cotransduction: 10% [8]
Synonyms:zie-296::Tn10

tna::Tn10

Linked marker

WP72[9]

  • tryptophanase minus
  • tetracycline resistant
  • est. P1 cotransduction: 85%
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10235

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10235

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000229

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0231

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012103

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DnaA

Synonyms

chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator[1], B3702[2][1], DnaA[2][1], DNA biosynthesis; DnaA-ATP initiates chromosome replication; can be transcription regulator[2][1] , ECK3694, JW3679, b3702

Product description

DnaA transcriptional dual regulator[2][3]

DNA synthesis initiator and global transcription regulator; binds DNA at DnaA boxes, binds cardiolipin and other acidic phospholipids, binds ATP[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0006275

regulation of DNA replication

PMID:12068813

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006260

DNA replication

PMID:12068813

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

PMID:10551881

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

P

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010921

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aspS

PMID:15690043

Experiment(s):EBI-883418

Protein

cca

PMID:15690043

Experiment(s):EBI-883418

Protein

dnaJ

PMID:15690043

Experiment(s):EBI-883418, EBI-890702

Protein

lpdA

PMID:15690043

Experiment(s):EBI-883418

Protein

nadE

PMID:15690043

Experiment(s):EBI-883418

Protein

rplB

PMID:15690043

Experiment(s):EBI-883418, EBI-890702

Protein

rplD

PMID:15690043

Experiment(s):EBI-883418

Protein

rpsB

PMID:15690043

Experiment(s):EBI-883418, EBI-890702

Protein

tufA

PMID:15690043

Experiment(s):EBI-883418

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1146527

Protein

yfdS

PMID:16606699

Experiment(s):EBI-1146527

Protein

yibL

PMID:16606699

Experiment(s):EBI-1146527

Protein

rpsG

PMID:16606699

Experiment(s):EBI-1146527

Protein

groL

PMID:16606699

Experiment(s):EBI-1146527

Protein

dgt

PMID:16606699

Experiment(s):EBI-1146527

Protein

creC

PMID:15690043

Experiment(s):EBI-890702

Protein

rplC

PMID:15690043

Experiment(s):EBI-890702

Protein

rplL

PMID:15690043

Experiment(s):EBI-890702

Protein

rplN

PMID:15690043

Experiment(s):EBI-890702

Protein

rplO

PMID:15690043

Experiment(s):EBI-890702

Protein

rplP

PMID:15690043

Experiment(s):EBI-890702

Protein

rplV

PMID:15690043

Experiment(s):EBI-890702

Protein

rplW

PMID:15690043

Experiment(s):EBI-890702

Protein

rplX

PMID:15690043

Experiment(s):EBI-890702

Protein

rpmB

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsC

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsE

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsG

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsI

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsM

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsN

PMID:15690043

Experiment(s):EBI-890702

Protein

rpsT

PMID:15690043

Experiment(s):EBI-890702

Protein

ynhG

PMID:15690043

Experiment(s):EBI-890702

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.5

Protein

rpsT

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplO

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsD

PMID:19402753

LCMS(ID Probability):95.6 MALDI(Z-score):19.412920

Protein

rpsM

PMID:19402753

LCMS(ID Probability):99.6

Protein

nadE

PMID:19402753

MALDI(Z-score):23.368207

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplB

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.312848

Protein

tufB

PMID:19402753

MALDI(Z-score):22.493646

Protein

rplX

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsC

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):6.532571

Protein

ynhG

PMID:19402753

LCMS(ID Probability):99.0

Protein

creC

PMID:19402753

LCMS(ID Probability):99.0

Protein

dnaJ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.405523

Protein

rplD

PMID:19402753

LCMS(ID Probability):96.5 MALDI(Z-score):23.806084

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Dps

PMID:18284581

Affinity chromatography and solid phase binding assay

Protein

Hda

PMID:11483528


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSLSLWQQCL ARLQDELPAT EFSMWIRPLQ AELSDNTLAL YAPNRFVLDW VRDKYLNNIN
GLLTSFCGAD APQLRFEVGT KPVTQTPQAA VTSNVAAPAQ VAQTQPQRAA PSTRSGWDNV
PAPAEPTYRS NVNVKHTFDN FVEGKSNQLA RAAARQVADN PGGAYNPLFL YGGTGLGKTH
LLHAVGNGIM ARKPNAKVVY MHSERFVQDM VKALQNNAIE EFKRYYRSVD ALLIDDIQFF
ANKERSQEEF FHTFNALLEG NQQIILTSDR YPKEINGVED RLKSRFGWGL TVAIEPPELE
TRVAILMKKA DENDIRLPGE VAFFIAKRLR SNVRELEGAL NRVIANANFT GRAITIDFVR
EALRDLLALQ EKLVTIDNIQ KTVAEYYKIK VADLLSKRRS RSVARPRQMA MALAKELTNH
SLPEIGDAFG GRDHTTVLHA CRKIEQLREE SHDIKEDFSN LIRTLSS
Length

467

Mol. Wt

52.551 kDa

pI

8.9 (calculated)

Extinction coefficient

43,890 - 44,265 (calc based on 11 Y, 5 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

132..350

PF00308 Bacterial dnaA protein

PMID:19920124

Domain

375..444

PF08299 domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dnaA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131570

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012103

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P03004

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10235

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10235

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000229

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0231

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.12E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

27.88+/-0.331

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.135067712

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

840

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

290

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

343

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dnaAN-recF

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3881732..3881772 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3702 (EcoliWiki Page)

NCBI GEO profiles for dnaA

microarray

GenExpDB:b3702 (EcoliWiki Page)

Summary of data for dnaA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to dnaA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10235

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0231

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3702

EcoGene

EcoGene:EG10235

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000229

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012103

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000026935 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000018624 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000023311 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

DNAA

From SHIGELLACYC

E. coli O157

DNAA

From ECOO157CYC

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<protect></protect>

Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00308 Bacterial dnaA protein

Pfam (EcoliWiki Page)

PF08299 domain


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Ohmori, H et al. (1984) Structural analysis of the dnaA and dnaN genes of Escherichia coli. Gene 28 159-70 PubMed
  6. ↑ Kellenberger-Gujer, G et al. (1978) A cold sensitive dnaA mutant of E. coli which overinitiates chromosome replication at low temperature. Mol. Gen. Genet. 162 9-16 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  9. ↑ Schaus, N et al. (1981) Isolation and characterization of amber mutations in gene dnaA of escherichia coli K-12. J. Bacteriol. 145 904-13 PubMed

Categories

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