dnaA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DnaA

Synonyms

chromosomal replication initiator protein DnaA, DNA-binding transcriptional dual regulator[1], B3702[2][1], DnaA[2][1], DNA biosynthesis; DnaA-ATP initiates chromosome replication; can be transcription regulator[2][1] , ECK3694, JW3679, b3702

Product description

DnaA transcriptional dual regulator[2][3]

DNA synthesis initiator and global transcription regulator; binds DNA at DnaA boxes, binds cardiolipin and other acidic phospholipids, binds ATP[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006275

regulation of DNA replication

PMID:12068813[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006260

DNA replication

PMID:12068813[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0003688

DNA replication origin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

PMID:10551881[6]

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

P

Seeded from EcoCyc (v14.0)

complete

GO:0006270

DNA-dependent DNA replication initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00377

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001957

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

P

Seeded from EcoCyc (v14.0)

complete

GO:0006275

regulation of DNA replication

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018312

P

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010921

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013159

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aspS

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

cca

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

dnaJ

PMID:15690043[7]

Experiment(s):EBI-883418, EBI-890702

Protein

lpdA

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

nadE

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

rplB

PMID:15690043[7]

Experiment(s):EBI-883418, EBI-890702

Protein

rplD

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

rpsB

PMID:15690043[7]

Experiment(s):EBI-883418, EBI-890702

Protein

tufA

PMID:15690043[7]

Experiment(s):EBI-883418

Protein

rpsI

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

yfdS

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

yibL

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

rpsG

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

groL

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

dgt

PMID:16606699[8]

Experiment(s):EBI-1146527

Protein

creC

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplC

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplL

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplN

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplO

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplP

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplV

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplW

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplX

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpmB

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsC

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsE

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsG

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsI

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsJ

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsM

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsN

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rpsT

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

ynhG

PMID:15690043[7]

Experiment(s):EBI-890702

Protein

rplP

PMID:19402753[9]

LCMS(ID Probability):99.5

Protein

rpsT

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplO

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplN

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsD

PMID:19402753[9]

LCMS(ID Probability):95.6 MALDI(Z-score):19.412920

Protein

rpsM

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

nadE

PMID:19402753[9]

MALDI(Z-score):23.368207

Protein

rpsJ

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rplB

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):17.312848

Protein

tufB

PMID:19402753[9]

MALDI(Z-score):22.493646

Protein

rplX

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

rpsC

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):6.532571

Protein

ynhG

PMID:19402753[9]

LCMS(ID Probability):99.0

Protein

creC

PMID:19402753[9]

LCMS(ID Probability):99.0

Protein

dnaJ

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):17.405523

Protein

rplD

PMID:19402753[9]

LCMS(ID Probability):96.5 MALDI(Z-score):23.806084

Protein

rplL

PMID:19402753[9]

LCMS(ID Probability):99.6

Protein

Dps

PMID:18284581[10]

Affinity chromatography and solid phase binding assay

Protein

Hda

PMID:11483528[11]


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSLSLWQQCL ARLQDELPAT EFSMWIRPLQ AELSDNTLAL YAPNRFVLDW VRDKYLNNIN
GLLTSFCGAD APQLRFEVGT KPVTQTPQAA VTSNVAAPAQ VAQTQPQRAA PSTRSGWDNV
PAPAEPTYRS NVNVKHTFDN FVEGKSNQLA RAAARQVADN PGGAYNPLFL YGGTGLGKTH
LLHAVGNGIM ARKPNAKVVY MHSERFVQDM VKALQNNAIE EFKRYYRSVD ALLIDDIQFF
ANKERSQEEF FHTFNALLEG NQQIILTSDR YPKEINGVED RLKSRFGWGL TVAIEPPELE
TRVAILMKKA DENDIRLPGE VAFFIAKRLR SNVRELEGAL NRVIANANFT GRAITIDFVR
EALRDLLALQ EKLVTIDNIQ KTVAEYYKIK VADLLSKRRS RSVARPRQMA MALAKELTNH
SLPEIGDAFG GRDHTTVLHA CRKIEQLREE SHDIKEDFSN LIRTLSS
Length

467

Mol. Wt

52.551 kDa

pI

8.9 (calculated)

Extinction coefficient

43,890 - 44,265 (calc based on 11 Y, 5 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

132..350

PF00308 Bacterial dnaA protein

PMID:19920124[12]

Domain

375..444

PF08299 domain

PMID:19920124[12]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dnaA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131570

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012103

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P03004

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10235

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10235

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948217

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000229

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0231

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Ogawa, T et al. (2002) The datA locus predominantly contributes to the initiator titration mechanism in the control of replication initiation in Escherichia coli. Mol. Microbiol. 44 1367-75 PubMed
  6. ↑ Azam, TA & Ishihama, A (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J. Biol. Chem. 274 33105-13 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 7.23 7.24 7.25 7.26 7.27 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 8.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ Chodavarapu, S et al. (2008) Escherichia coli Dps interacts with DnaA protein to impede initiation: a model of adaptive mutation. Mol. Microbiol. 67 1331-46 PubMed
  11. ↑ Kato , J & Katayama, T (2001) Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli. EMBO J. 20 4253-62 PubMed
  12. ↑ 12.0 12.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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