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Standard Name	dgt
Gene Synonym(s)	ECK0159, b0160, JW0156, optA^[1], optA
Product Desc.	Dgt^[2]^[3]; Component of deoxyguanosinetriphosphate triphosphohydrolase^[2]^[3] Deoxyguanosine 5'-triphosphate triphosphohydrolase^[4]
Product Synonyms(s)	deoxyguanosine triphosphate triphosphohydrolase^[1], B0160^[2]^[1], Dgt^[2]^[1], OptA^[2]^[1] , ECK0159, JW0156, optA, b0160
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dgt^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dgt
Mnemonic	dGTP triphosphohydrolase
Synonyms	ECK0159, b0160, JW0156, optA^[1], optA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	3.86 minutes	MG1655: 179237..180754 <gbrowseImage> name=NC_000913:179237..180754 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 182079..183596 <gbrowseImage> name=NC_012967:182079..183596 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 179236..180753 <gbrowseImage> name=NC_012759:179236..180753 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 179237..180754 <gbrowseImage> name=NC_007779:179237..180754 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 153341..154858 <gbrowseImage> name=NC_010473:153341..154858 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	179240	Edman degradation	PMID:2826481 PMID:2833745
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
dgt(del) (Keio:JW0156)	deletion	deletion		PMID:16738554	Shigen CGSC8423^[5]
dgt::Tn5KAN-2 (FB20028)	Insertion at nt 172 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20028	does not contain pKD46
dgt-774(del)::kan				PMID:16738554	CGSC:100590
dgt::mini-Tn10cam			mutator activity (increased frequency of A^.T->G^.C transitions and G^.C->C^.G transversions)	PMID:18776019		Fig. 1 and Table 2
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0156	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCACAGATTGATTTCCGAAA Primer 2:CCTTGTTCTACGGCCATCAGACG
9H2	Kohara Phage	Genobase	PMID:3038334
4E4	Kohara Phage	Genobase	PMID:3038334
21C8	Kohara Phage	Genobase	PMID:3038334
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[5]	est. P1 cotransduction: 30% ^[6]
yafC502::Tn10	Linked marker	CAG18436 = CGSC7328^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zae-502::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10225	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10225	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947177	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000219	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0221	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000548	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Dgt
Synonyms	deoxyguanosine triphosphate triphosphohydrolase^[1], B0160^[2]^[1], Dgt^[2]^[1], OptA^[2]^[1] , ECK0159, JW0156, optA, b0160
Product description	Dgt^[2]^[3]; Component of deoxyguanosinetriphosphate triphosphohydrolase^[2]^[3] Deoxyguanosine 5'-triphosphate triphosphohydrolase^[4]
EC number (for enzymes)	3.1.5.1^[1]
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Notes

Deoxyguanosine triphosphate triphosphohydrolase was identified by Dr. Sylvy Kornberg as an inhibitor of in vitro DNA replication in the Kornberg lab.^[7]

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00030	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006261	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020779	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008832	dGTPase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00030	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008832	dGTPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006261	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008832	dGTPase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR020779	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008832	dGTPase activity	PMID:2833745	IDA: Inferred from Direct Assay		F		complete
	GO:0008832	dGTPase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.1.5.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046039	GTP metabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006261	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003924	GTPase activity	PMID:2826481	IDA: Inferred from Direct Assay		F		complete
	GO:0030145	manganese ion binding	PMID:2826481	IDA: Inferred from Direct Assay		F		complete
	GO:0050897	cobalt ion binding	PMID:2826481	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of deoxyguanosinetriphosphate triphosphohydrolase	could be indirect
Protein	yacH		PMID:16606699	Experiment(s):EBI-1135747
Protein	groL		PMID:16606699	Experiment(s):EBI-1135747
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAQIDFRKKI NWHRRYRSPQ GVKTEHEILR IFESDRGRII NSPAIRRLQQ KTQVFPLERN AAVRTRLTHS MEVQQVGRYI AKEILSRLKE LKLLEAYGLD ELTGPFESIV EMSCLMHDIG NPPFGHFGEA AINDWFRQRL HPEDAESQPL TDDRCSVAAL RLRDGEEPLN ELRRKIRQDL CHFEGNAQGI RLVHTLMRMN LTWAQVGGIL KYTRPAWWRG ETPETHHYLM KKPGYYLSEE AYIARLRKEL NLALYSRFPL TWIMEAADDI SYCVADLEDA VEKRIFTVEQ LYHHLHEAWG QHEKGSLFSL VVENAWEKSR SNSLSRSTED QFFMYLRVNT LNKLVPYAAQ RFIDNLPAIF AGTFNHALLE DASECSDLLK LYKNVAVKHV FSHPDVERLE LQGYRVISGL LEIYRPLLSL SLSDFTELVE KERVKRFPIE SRLFHKLSTR HRLAYVEAVS KLPSDSPEFP LWEYYYRCRL LQDYISGMTD LYAWDEYRRL MAVEQ
Length	505
Mol. Wt	59.384 kDa
pI	7.5 (calculated)
Extinction coefficient	89,270 - 90,020 (calc based on 23 Y, 10 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P15723
Domain	66..183	PF01966 HD domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dgt taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128153	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947177	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000548	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P15723	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10225	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10225	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947177	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000219	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0221	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	95	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	129	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	109	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dgt
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:179217..179257 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0160 (EcoliWiki Page)	NCBI GEO profiles for dgt
microarray	GenExpDB:b0160 (EcoliWiki Page)	Summary of data for dgt from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (179062..179277) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ18; Well:B3^[8]
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Notes

Accessions Related to dgt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10225	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0221	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0160
EcoGene	EcoGene:EG10225	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000219	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000548	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	DGT	From SHIGELLACYC
E. coli O157	DGT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01966 HD domain
Panther (EcoliWiki Page)	PTHR11373:SF18
Superfamily (EcoliWiki Page)	SUPERFAMILY:109604
EcoCyc	EcoCyc:EG10225	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10225	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000219	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0221	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000548	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ KORNBERG, SR et al. (1958) Enzymatic cleavage of deoxyguanosine triphosphate to deoxyguanosine and tripolyphosphate. J. Biol. Chem. 233 159-62 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

dgt:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dgt Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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