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Gene |

Product(s) |

Expression|

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Standard Name	cynS
Gene Synonym(s)	ECK0337, b0340, JW0331, cnt, cyaN^[1]
Product Desc.	cyanase^[2]^[3]; Component of cyanase^[2]; cyanase dimer^[3]; cyanase^[3] Cyanate aminohydrolase^[4]
Product Synonyms(s)	cyanate aminohydrolase^[1], B0340^[2]^[1], Cnt^[2]^[1], CyaN^[2]^[1], CynS^[2]^[1], cyanate hydrolase^[2]^[1], cyanase^[2]^[1], cyanate C-N-lyase^[2]^[1] , cnt, ECK0337, JW0331, b0340
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cynTSX^[2], OP00193, cynTS^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cynS
Mnemonic	Cyanase; cyanate metabolism
Synonyms	ECK0337, b0340, JW0331, cnt, cyaN^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	7.73 minutes	MG1655: 358713..359183 <gbrowseImage> name=NC_000913:358713..359183 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 332045..332515 <gbrowseImage> name=NC_012967:332045..332515 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
W3110		W3110: 358713..359183 <gbrowseImage> name=NC_007779:358713..359183 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1378249..1378719 <gbrowseImage> name=NC_010473:1378249..1378719 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	358713	Edman degradation	PMID:6336748
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

cynS(del) (Keio:JW0331)

deletion

PMID:16738554

Shigen

CGSC8526^[5]

cynS781(del)::kan

PMID:16738554

CGSC:100466

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0331	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATTCAGTCACAAATTAACCG Primer 2:CCGAACGGTTTGGTCGGCAGATA
10A6	Kohara Phage	Genobase	PMID:3038334
proA81::Tn10	Linked marker	CAG18447 = CGSC7330^[5]	est. P1 cotransduction: % ^[6]
lacI3042::Tn10	Linked marker	CAG18439 = CGSC7334^[5]	est. P1 cotransduction: 77% ^[6] Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10175	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10175	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000170	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948998	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0172	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CynS
Synonyms	cyanate aminohydrolase^[1], B0340^[2]^[1], Cnt^[2]^[1], CyaN^[2]^[1], CynS^[2]^[1], cyanate hydrolase^[2]^[1], cyanase^[2]^[1], cyanate C-N-lyase^[2]^[1] , cnt, ECK0337, JW0331, b0340
Product description	cyanase^[2]^[3]; Component of cyanase^[2]; cyanase dimer^[3]; cyanase^[3] Cyanate aminohydrolase^[4]
EC number (for enzymes)	4.2.1.104^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	PMID:8083164	IDA: Inferred from Direct Assay		C	Seeded from Riley et al 2006 ^[1].	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010982	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008824	cyanate hydratase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00535	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008824	cyanate hydratase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003712	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008824	cyanate hydratase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1.104	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016836	hydro-lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008076	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of cyanase	could be indirect
Protein	prs		PMID:16606699	Experiment(s):EBI-1136296
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1136296
Protein	gshB		PMID:16606699	Experiment(s):EBI-1136296
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MIQSQINRNI RLDLADAILL SKAKKDLSFA EIADGTGLAE AFVTAALLGQ QALPADAARL VGAKLDLDED SILLLQMIPL RGCIDDRIPT DPTMYRFYEM LQVYGTTLKA LVHEKFGDGI ISAINFKLDV KKVADPEGGE RAVITLDGKY LPTKPF
Length	156
Mol. Wt	17.049 kDa
pI	4.8 (calculated)
Extinction coefficient	5,960 - 6,085 (calc based on 4 Y, 0 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	83..156	PF02560 Cyanate lyase C-terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cynS taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128325	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948998	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001173	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00816	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10175	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10175	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948998	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000170	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0172	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	96	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	62	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	49^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cynTSX
cynTS
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:358693..358733 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0340 (EcoliWiki Page)	NCBI GEO profiles for cynS
microarray	GenExpDB:b0340 (EcoliWiki Page)	Summary of data for cynS from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to cynS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10175	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0172	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0340
EcoGene	EcoGene:EG10175	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000170	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001173	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Arabidopsis thaliana	AT3G23490 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q7XDI1 (score: 1.016) Q9FWK4 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	CYNS	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02560 Cyanate lyase C-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:47413
Superfamily (EcoliWiki Page)	SUPERFAMILY:55234
EcoCyc	EcoCyc:EG10175	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10175	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000170	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0172	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001173	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 ^1.16 ^1.17 ^1.18 ^1.19 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 ^2.17 ^2.18 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

cynS:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cynS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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