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Product(s) |

Expression|

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Standard Name	cyaA
Gene Synonym(s)	ECK3800, b3806, JW3778, cya^[1], cya
Product Desc.	Adenylate cyclase^[2]
Product Synonyms(s)	adenylate cyclase^[1], B3806^[3]^[1], Cya^[3]^[1], CyaA^[3]^[1] , cya, ECK3800, JW3778, b3806
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): cyaA^[3], OP00023, cya
Regulation/Activity
Quick Links
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Notes

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Polyamine modulon.^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cyaA
Mnemonic	Cyclase, adenylate
Synonyms	ECK3800, b3806, JW3778, cya^[1], cya
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	85.98 minutes	MG1655: 3989176..3991722 <gbrowseImage> name=NC_000913:3989176..3991722 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3952983..3955529 <gbrowseImage> name=NC_012967:3952983..3955529 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3878845..3881391 <gbrowseImage> name=NC_012759:3878845..3881391 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3645528..3642982 <gbrowseImage> name=NC_007779:3642982..3645528 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
cyaA (Keio:JW3778)	deletion	deletion			PMID:16738554	Shigen CGSC10748^[4]
cyaA::Tn5KAN-I-SceI (FB21464)	Insertion at nt 1522 in Minus orientation				PMID:15262929	E. coli Genome Project:FB21464	does not contain pKD46
cyaA::Tn5KAN-I-SceI (FB21465)	Insertion at nt 1522 in Minus orientation				PMID:15262929	E. coli Genome Project:FB21465	contains pKD46
cyaA2(del)					PMID:4583241 PMID:2022622	CGSC:6317
cyaA854(del)					PMID:2670905	CGSC:7791
cyaA1					PMID:173710	CGSC:11875
cyaA1400(del)::kan					PMID:2022622	CGSC:22922
cyaA201(del)					PMID:4583241 PMID:2022622	CGSC:27406
cyaA1404(del)::FRT					PMID:10829079	CGSC:64880
cyaA1403(del)::kan					PMID:10829079	CGSC:65380
cyaA161					PMID:9495769	CGSC:92361
cyaA751(del)::kan					PMID:16738554	CGSC:100456
cya(del)	deletion	deletion	Auxotrophies	Mutation inhibits stationary phase SOS induction of the yebG gene (i.e. dependent on intracellular cAMP levels), fig 3.	PMID:10474193
cya in strain L176			Sensitivity to	sensitivity to streptozotocin	PMID:6450313	strain L176	figure 3
cya (del) crp (del)			Sensitivity to	sensitivity to streptozotocin	PMID:6450313	Strain: CA8445	table 2 and figure 3
cya crp* relA⁺			Resistant to	crp allele that modified cyclic AMP receptor proteins, which acts independently of cyclic AMP, is resistant to smg (serine+methionine+glycine) in a relA background.	PMID:230407	Experimental strain: BM6163	table 4
cya(del)			Resistant to	Resistance to UV irridiation (254nm).	PMID:1379686	Strain: GE1068	See figure 1
Salmonella typhimurium LT2 cya-deficient			Resistant to	partially resistant to 22 antibiotics (including fosfomycin, nalidixic acid, and streptomycin)	PMID:201606
cya in strain NCR-C57			Resistant to	Resistance to T6 Phage	PMID:227838	Experiential Strain: NCR-C57 Parental Strain: NCR-701	Table 3
cyaA751(del)::FRTKanFRT	deletion		Mutagenesis Rate	Decrease in Stress Induced Mutagenesis (SIM).	PMID:23224554	Parent Strain: 23224554 Experimental Strain: SMR11998	The mutation conferred a significant decrease in SIM with a reduction in mutant frequency by 67 to 89 percent. See table S3 for full experimental data.
cya-408			Resistant to	Resistant to Fosfomycin	PMID:201606	Strain: TA3301	Strain Parent: LT2 Strain donor: TA2301
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3778	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTACCTCTATATTGAGACTCT Primer 2:CCaGAAAAATATTGCTGTAATAG
12G1	Kohara Phage	Genobase	PMID:3038334
ilvD500::Tn10	Linked marker	CAG18431 = CGSC7462^[4]	est. P1 cotransduction: 23% ^[5] Synonyms:ilv500::Tn10
metE3079::Tn10	Linked marker	CAG18491 = CGSC7464^[4]	est. P1 cotransduction: 49% ^[5] The Tn10 in CAG13491 is inserted in the metE regulatory region, but causes a Met^- phenotype.
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10170	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10170	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000166	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947755	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0168	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012432	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CyaA
Synonyms	adenylate cyclase^[1], B3806^[3]^[1], Cya^[3]^[1], CyaA^[3]^[1] , cya, ECK3800, JW3778, b3806
Product description	Adenylate cyclase^[2]
EC number (for enzymes)	4.6.1.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004016	adenylate cyclase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000274	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004016	adenylate cyclase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.6.1.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006171	cAMP biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000274	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006171	cAMP biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0115	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	dnaE	PMID:16606699	Experiment(s):EBI-1146708
Protein	rapA	PMID:16606699	Experiment(s):EBI-1146708
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MYLYIETLKQ RLDAINQLRV DRALAAMGPA FQQVYSLLPT LLHYHHPLMP GYLDGNVPKG ICLYTPDETQ RHYLNELELY RGMSVQDPPK GELPITGVYT MGSTSSVGQS CSSDLDIWVC HQSWLDSEER QLLQRKCSLL ENWAASLGVE VSFFLIDENR FRHNESGSLG GEDCGSTQHI LLLDEFYRTA VRLAGKRILW NMVPCDEEEH YDDYVMTLYA QGVLTPNEWL DLGGLSSLSA EEYFGASLWQ LYKSIDSPYK AVLKTLLLEA YSWEYPNPRL LAKDIKQRLH DGEIVSFGLD PYCMMLERVT EYLTAIEDFT RLDLVRRCFY LKVCEKLSRE RACVGWRRAV LSQLVSEWGW DEARLAMLDN RANWKIDQVR EAHNELLDAM MQSYRNLIRF ARRNNLSVSA SPQDIGVLTR KLYAAFEALP GKVTLVNPQI SPDLSEPNLT FIYVPPGRAN RSGWYLYNRA PNIESIISHQ PLEYNRYLNK LVAWAWFNGL LTSRTRLYIK GNGIVDLPKL QEMVADVSHH FPLRLPAPTP KALYSPCEIR HLAIIVNLEY DPTAAFRNQV VHFDFRKLDV FSFGENQNCL VGSVDLLYRN SWNEVRTLHF NGEQSMIEAL KTILGKMHQD AAPPDSVEVF CYSQHLRGLI RTRVQQLVSE CIELRLSSTR QETGRFKALR VSGQTWGLFF ERLNVSVQKL ENAIEFYGAI SHNKLHGLSV QVETNHVKLP AVVDGFASEG IIQFFFEETQ DENGFNIYIL DESNRVEVYH HCEGSKEELV RDVSRFYSSS HDRFTYGSSF INFNLPQFYQ IVKVDGREQV IPFRTKSIGN MPPANQDHDT PLLQQYFS
Length	848
Mol. Wt	97.587 kDa
pI	6.2 (calculated)
Extinction coefficient	147,600 - 149,475 (calc based on 40 Y, 16 W, and 15 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Modification Site	407	phosphorylation site at S407	probability greater than 75%	PMID:17938405
Modification Site	409	phosphorylation site at S409	probability less than 75%	PMID:17938405
Domain	1..830	PF01295 Adenylate cyclase, class-I		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=cyaA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131658	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947755	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012432	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P00936	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10170	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10170	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947755	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000166	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0168	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	372	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	263	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	342	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
cyaA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3989156..3989196 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3806 (EcoliWiki Page)	NCBI GEO profiles for cyaA
microarray	GenExpDB:b3806 (EcoliWiki Page)	Summary of data for cyaA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3988326..3988849) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:C7^[6]
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Notes

Accessions Related to cyaA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10170	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0168	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3806
EcoGene	EcoGene:EG10170	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000166	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012432	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	CYAA	From SHIGELLACYC
E. coli O157	CYAA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01295 Adenylate cyclase, class-I
EcoCyc	EcoCyc:EG10170	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10170	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000166	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0168	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012432	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

cyaA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to cyaA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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