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Expression|

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Standard Name	cra
Gene Synonym(s)	ECK0081, b0080, JW0078, cra, fruC, shl, serR?, fruS?, fruR
Product Desc.	FruR transcriptional dual regulator; Component of FruR transcriptional dual regulator; FruR-fructose-1,6-bisphosphate; FruR-fructose-1-phosphate Regulatory gene for fru operon, other catabolite-regulated genes
Product Synonyms(s)	DNA-binding transcriptional dual regulator, B0080, Shl, FruC, FruR, Cra , cra, ECK0081, fruC, fruS?, JW0078, serR?, shl, b0080, Cra
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fruR^[1]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	cra
Mnemonic	Fructose
Synonyms	ECK0081, b0080, JW0078, cra, fruC, shl, serR?, fruS?, fruR
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.9 minutes	MG1655: 88028..89032 <gbrowseImage> name=NC_000913:88028..89032 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 90832..91836 <gbrowseImage> name=NC_012967:90832..91836 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 88027..89031 <gbrowseImage> name=NC_012759:88027..89031 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 88028..89032 <gbrowseImage> name=NC_007779:88028..89032 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 62379..63136 <gbrowseImage> name=NC_010473:62379..63136 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
fruR(del) (Keio:JW0078)	deletion	deletion			PMID:16738554	Shigen CGSC8378^[2]
fruR(del)::kan	deletion		Biolog:respiration	unable to respire DL-a-Glycerol	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire Acetate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire a-Hydroxybutyrate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire a-Ketobutyrate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire a-Ketoglutarate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire DL-Lactate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire Succinate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire Bromosuccinate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire L-Alanine	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire L-Alanyl-glycine	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire L-Asparagine	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire L-Aspartate	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire D-Serine	PMID:16095938
fruR(del)::kan	deletion		Biolog:respiration	unable to respire L-Serine	PMID:16095938
fruR11::Tn10						CGSC:47453
fruR12::kan					PMID:8577250 PMID:9084162	CGSC:47646
fruR786(del)::kan					PMID:16738554	CGSC:101024
CAG45114 fruR786(del)::FRTKanFRT	deletion		Sigma^E activity	Decrease in sigma^E activity	PMID:23224554	Parental Strain: CAG45114 Experimental Strain: SMR15253	See table S11 for full experimental data.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0078	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAACTGGATGAAATCGCTCG Primer 2:CCGCTACGGCTGAGCACGCCGCG
6C1	Kohara Phage	Genobase	PMID:3038334
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[2]	est. P1 cotransduction: 86% ^[3] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
zad-220::Tn10	Linked marker	CAG12025 = CGSC7326^[2]	est. P1 cotransduction: 4% ^[3]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (EcoliWiki Page)	GeneID:944804	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Cra
Synonyms	DNA-binding transcriptional dual regulator, B0080, Shl, FruC, FruR, Cra , cra, ECK0081, fruC, fruS?, JW0078, serR?, shl, b0080, Cra
Product description	FruR transcriptional dual regulator; Component of FruR transcriptional dual regulator; FruR-fructose-1,6-bisphosphate; FruR-fructose-1-phosphate Regulatory gene for fru operon, other catabolite-regulated genes
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010982	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012781	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0238	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003700	transcription factor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006350	transcription	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0804	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000843	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012781	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009750	response to fructose stimulus	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012781	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of FruR transcriptional dual regulator	could be indirect
Protein	matC		PMID:16606699	Experiment(s):EBI-1135523
Protein	groL		PMID:16606699	Experiment(s):EBI-1135523
Protein	nadE		PMID:16606699	Experiment(s):EBI-1135523
Protein	dnaK		PMID:16606699	Experiment(s):EBI-1135523
Protein	hldD		PMID:15690043	Experiment(s):EBI-889721
Protein	rpsC		PMID:15690043	Experiment(s):EBI-889721
Protein	tufA		PMID:15690043	Experiment(s):EBI-889721
Protein	ssb		PMID:19402753	MALDI(Z-score):26.363338
Protein	fadB		PMID:19402753	MALDI(Z-score):21.861324
Protein	Subunits of FruR-fructose-1,6-bisphosphate	could be indirect
Protein	Subunits of FruR-fructose-1-phosphate	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKLDEIARLA GVSRTTASYV INGKAKQYRV SDKTVEKVMA VVREHNYHPN AVAAGLRAGR TRSIGLVIPD LENTSYTRIA NYLERQARQR GYQLLIACSE DQPDNEMRCI EHLLQRQVDA IIVSTSLPPE HPFYQRWAND PFPIVALDRA LDREHFTSVV GADQDDAEML AEELRKFPAE TVLYLGALPE LSVSFLREQG FRTAWKDDPR EVHFLYANSY EREAAAQLFE KWLETHPMPQ ALFTTSFALL QGVMDVTLRR DGKLPSDLAI ATFGDNELLD FLQCPVLAVA QRHRDVAERV LEIVLASLDE PRKPKPGLTR IKRNLYRRGV LSRS
Length	334
Mol. Wt	37.999 kDa
pI	7.0 (calculated)
Extinction coefficient	32,890 - 33,265 (calc based on 11 Y, 3 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	2..50	PF00356 Bacterial regulatory proteins, lacI family	PMID:19920124
Domain	61..327	PF00532 family	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fruR taxon=12017 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128073	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:944804	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACP1	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:944804	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	28.733+/-0.155	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.043824701	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	987	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	314	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	683	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fruR
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:88008..88048 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0080 (EcoliWiki Page)	NCBI GEO profiles for fruR
microarray	GenExpDB:b0080 (EcoliWiki Page)	Summary of data for fruR from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fruR Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0080
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FRUR	From SHIGELLACYC
E. coli O157	FRUR	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00356 Bacterial regulatory proteins, lacI family
Superfamily (EcoliWiki Page)	SUPERFAMILY:47413
Superfamily (EcoliWiki Page)	SUPERFAMILY:53822
Pfam (EcoliWiki Page)	PF00532 family
EcoCyc	EcoCyc:	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^2.0 ^2.1 ^2.2 CGSC: The Coli Genetics Stock Center
↑ ^3.0 ^3.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

cra:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fruR Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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